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Application Stories

Your RNA NGS data analysis service

Watch the movie directly on Youtube

Introducing XploreRNA

The online platform for accelerated RNA NGS data analysis and validation.

XploreRNA is the most capable online RNA NGS data analysis platform ever. It's easy and fast to use, and available 24/7.

XploreRNA addresses the bottlenecks and workflow inefficiencies faced by researchers to help advance research – without compromising quality.

Go from FASTQ files to results in just 1 week

Upload your raw sequencing data

Data QC

Filtering, mapping, supervised and unsupervised NGS data analysis

Xplore your results using the interactive Gene Sorting Wizard

Validate candidate genes

Easy to use

It only takes 5 minutes to set up your project in XploreRNA. Simply upload your data files, and let the XploreRNA data analysis pipeline conduct the analysis. Results are presented in a detailed report.

Analysis includes everything from Data QC, Mapping, Normalization, Differential expression analysis and statistical analysis, to Gene Ontology Enrichment analysis.

Validation made easy

XploreRNA also makes validation easy by the Gene Sorting Wizard that allows you to sort and filter gene transcripts to validate.

What sequencing data can be analyzed by XploreRNA?

  • Any RNA sequencing data can be analyzed by XploreRNA: mRNA, whole transcriptome, microRNA or small RNA.
  • Raw data should be FASTQ files from either an Illumina or Ion Torrent Sequencer.
  • XploreRNA analyzes data generated using a range of different library preparation methods including Illumina TruSeq Stranded Total RNA Library Prep Kit, Illumina TruSeq Stranded mRNA Library Prep Kit, NEBNext® Ultra™ RNA Library Prep Kit for Illumina, Illumina ScriptSeq RNA-Seq Library Prep Kit, and Thermo Fisher Ion Total RNA-Seq Kit v2. If you are using a kit that is not included above, please contact us and we will do our best to help you.
  • XploreRNA analyzes data from 20 species: Arabidopsis thaliana, Bos Taurus, Caenorhabditis elegans, Canis familiaris, Danio rerio, Drosophila melanogaster, Equus caballus, Gallus gallus, Glycine max, Homo sapiens, Macaca mulatta, Mus musculus, Oryza sativa japonica, Pan troglodytes, Rattus norvegicus, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Sorghum bicolor, Sus scrofa, Zea mays. If you work with an organism not on the list, please contact us.


XploreRNA demo report

No more waiting for results

Go to XploreRNA online analysis service

NGS results report

Download XploreRNA report example

NGS data analysis pipeline

View XploreRNA pipeline

Gene Sorting Wizard

View the Gene Sorting Wizard

The XploreRNA RNA NGS data analysis service includes:

  • Upload of your FASTQ files to a local secure Amazon-hosted server
  • Data QC
  • Filtering and mapping to the reference genome
  • Identification of splice junctions
  • Mapping results
  • Identification of known and novel transcripts
  • Data normalization
  • Differential expression analysis of all identified transcripts including statistical analysis
  • Gene Ontology Enrichment Analysis
  • Easy-to-read Data Analysis Report (pdf) including QC plots, heat map, PCA plot, transcripts differentially expressed between groups, statistical analysis, Volcano plot, GO enrichment analysis, and GO network plot
  • Access to the sophisticated Gene Sorting Wizard to explore group comparisons and filter differentially expressed transcripts according to all relevant criteria
  • All result files in downloadable format (.tsv, .BAM and .pdf)
  • High resolution figures in downloadable format (.pdf)
  • Easy ordering of custom products for validation and functional analysis with just one click (no need to upload any sequences as design is done automatically for LNA™ qPCR assays, microRNA inhibitors, mimics and ISH probes, as well as Antisense LNA™ GapmeRs for knockdown of any mRNA or lncRNA).
  • Data storage
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