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Toxicology Focus microRNA PCR Panel

PCR panels containing miRCURY LNA™ Universal RT microRNA PCR assays for all relevant toxicology-related microRNAs. Panels are available in ready-to-use 96 and 384 well plates – just add cDNA and PCR master mix. Ideal for microRNA biomarker detection in biofluids such as serum/plasma and urine.

Urine microRNA biomarkers for kidney damage

Read this white paper to learn about the discovery of microRNA toxicology biomarkers in urine, including strategies for normalization and data analysis.

Download paper
  • All relevant toxicology-related microRNAs for human, rat, dog and monkey biofluid samples in PCR Panels
  • Developed using in-house data from thousands of biofluid samples, from peer-reviewed publications and in collaboration with pharma companies
  • Ideally suited for profiling in biofluids. 20µL serum/plasma sufficient for full profiling
  • Sensitivity and specificity for accurate microRNA profiling
  • Fast and easy - ready-to-use format with a full profile in less than 3 hours
  • High compatibility – 96 and 384 well formats compatible with most real-time PCR instruments

MicroRNAs as organ damage biomarkers

MicroRNAs in biofluids, such as urine, serum/plasma and cerebrospinal fluid, have received much attention for their potential as minimally invasive biomarkers for a wide range of diseases and conditions. They have been found to be extraordinarily stable in commonly used clinical samples such as urine, serum and plasma. Learn more about microRNAs


Several recent studies have shown that tissue injury leads to the release of microRNAs into the surrounding extra-cellular space. Depending on the nature of the injury, increased amounts of tissue-specific microRNAs or other microRNAs can be detected in the biofluids of the individual. Learn more about microRNA in biofluids

The well-defined tissue-specific expression patterns of some microRNAs, their presence in biofluids and their conservation across species, make these microRNAs prime candidates for use as biomarkers for organ injury and toxicity.

Exiqon’s miRCURY LNA™ Universal RT microRNA PCR system offers the sensitivity and specificity needed for microRNA profiling from biofluids ─ 20 µl serum/plasma is sufficient for profiling on a PCR plate. This makes the system ideally suited for use in diagnostics.






Figure 1
Examples of applications of the panel. (Click to learn more)


A unique system developed specifically for microRNA profiling

The miRCURY LNA™ Universal RT microRNA PCR System offers the best available combination of performance and ease-of-use on the market because it unites two important features (Figure 2):
  • One cDNA reaction for all microRNAs – One single Universal first strand cDNA synthesis reaction is used as template, regardless of the number of microRNAs being profiled. This saves precious sample, reduces technical variation, means less pipetting and saves time in the laboratory.
  • Two LNA™-enhanced microRNA qPCR primers – Both qPCR primers are microRNA-specific and optimized with LNA™. LNA™ primers bind with high affinity, and are shorter than standard PCR primers, so that both primers can fit on the microRNA without overlapping. The result is unrivalled sensitivity and specificity, and extremely low background.

Unmatched sensitivity

Universal RT combined with LNA™-enhanced and Tm normalized primers enables accurate and reliable quantification of individual microRNAs from as little as 1 pg total RNA (Figure 3). In comparison to other microRNA real-time PCR systems using either stem-loop or standard DNA primers, the LNA™-enhanced primers offer significantly increased sensitivity, especially for AU-rich microRNAs.

Exceptional sensitivity as well as extremely low background enables accurate quantification of very low levels of microRNA without the need for pre-amplification. This makes the miRCURY LNA™ Universal RT microRNA PCR System suitable for all sample types, and especially samples with low RNA content e.g. biofluids such as serum/plasma.

Fully validated and optimized

All miRCURY LNA™ Universal RT microRNA PCR primer sets have been optimized for maximum sensitivity and thoroughly validated either by wet lab testing or in silico. In wet lab validation, over 95% of assays detect 10 microRNA copies or less in the PCR reaction (Figure 4). The primer sets have also been validated for specific amplification of the target and for minimal background signal.

Truly specific – no false positives

The incorporation of LNA™ in the qPCR primers facilitates the design of assays that can distinguish between microRNA sequences that differ by a single nucleotide. This makes it a truly specific microRNA qPCR platform – the only microRNA platform that gives no false positive signals (Figure 5). In addition, the assays can discriminate between mature microRNA sequences and precursor microRNA (Table 1).

Fast, easy and reproducible

Save time and effort in the laboratory with the 3 hour easy-to-follow protocol that minimizes pipetting. By using the same cDNA as template in all subsequent PCR reactions, the procedure is greatly simplified compared to systems that require microRNA-specific cDNA synthesis.

With fewer pipetting steps, technical variation is also reduced. As a result, it is possible to achieve extremely high reproducibility from day-to-day (Figure 6) and even site-to-site.

Flexible qPCR system

Tailor your experimental setup to your specific need using individual assays or fully flexible custom Pick-&-Mix panels . Using the same cDNA reaction, shift seamlessly between individual primers, pre-designed miRNome and Focus panels, or custom Pick-&-Mix panels.
Figure 1 Overview of the miRCURY LNA™ Universal RT PCR workflow
Overview of the miRCURY LNA™ Universal RT PCR workflow. (Click to learn more)

Figure 2 Schematic outline of the miRCURY LNA™ Universal RT microRNA PCR System
Schematic outline of the miRCURY LNA™ Universal RT microRNA PCR System. (Click to learn more)

Figure 3 Accurate quantitation from down to 1 pg total RNA starting material
Accurate quantitation from down to 1 pg total RNA starting material. (Click to learn more)

Figure 4 Serial dilution of hsa-miR-181a
Serial dilution of hsa-miR-181a. (Click to learn more)

Figure 5 Specificity results from the miRQC Study
Specificity results from the miRQC Study. (Click to learn more)

Table 1 Discrimination between mature and precursor microRNAs
Discrimination between mature and precursor microRNAs. (Click to learn more)

Figure 6 Excellent day-to-day reproducibility
Excellent day-to-day reproducibility. (Click to learn more)

Focus on the microRNAs relevant to your project

The Toxicology Focus microRNA PCR Panel includes an optimized set of microRNA PCR assays designed to investigate signs of organ damage in biofluids from human, rat, monkey and dog.

Selected assays include:
  • Organ-specific damage markers
  • Tissue-specific and /or enriched microRNAs
  • microRNAs that are highly expressed in many tissues
A considerable part of the selected assays were extracted from Exiqon’s sample database which contains microRNA expression data from thousands of biofluid samples. This ensures that scientists studying organ damage will have access to all the relevant microRNAs.


Coverage

Exiqon’s Toxicology Focus microRNA PCR Panel contains all relevant toxicology-related microRNAs in one PCR Panel. The panel is available in 96-well or 384-well (containing 4 panels) plates compatible with most PCR instruments (Figure 1). 

Download a list of all microRNAs included on the panel

Download a list of references for the microRNAs included on the panels





Figure 1
Instrument coverage of the panels. (Click to learn more)

Figure 2
microRNA coverage of the panel. (Click to learn more)

microRNA as disease and toxicology biomarkers

Due to their wide-ranging biological potential and the fact that microRNAs seem to be relatively stable in readily available biofluids, these small molecules are prime candidates for use as non-invasive biomarkers in molecular diagnostics of disease and other clinical conditions, such as organ damage. They can also be used in pre-clinical toxicology and drug safety assessments.

In a toxicology study, where a nephrotoxin that is known to cause acute renal failure through induction of proximal tubular damage was administered intraperitoneally in rats, urine samples were collected and the isolated RNA was profiled using Exiqon’s Toxicology Focus microRNA PCR Panel. Samples were collected over-night at day 3 and day 5 post nephrotoxin administration and rats were sacrificed at day 3 and 5 for histopathological evaluation (Figure 1).

MicroRNA expression was profiled using the miRCURY LNA™ Universal RT microRNA PCR System. Only assays with Cq values below 37 and that differed from their corresponding negative control with more than 4.8 Cq values were included in the analysis.

Unsupervised clustering analysis demonstrates that the microRNA profile changes significantly upon nephrotoxin administration and that the differences between the control and treated samples increases over time. Principal Component Analysis (PCA) illustrates that the samples separate in 3 distinct groups (Figure 2). A Heat-map of the 16 samples and 35 microRNAs that passed QC and cut-off criteria illustrate the expression level across all samples (Figure 3). Detailed fold changes for microRNAs rno-miR-21-5p, rno-miR-34a-5p and rno-miR-192-5p are shown in Figure 4.

Figure 1 Overview of the experimental design and level of kidney necrosis
Overview of the experimental design and level of kidney necrosis. (Click to learn more)

Figure 2 Clear separation of treatment and control groups using Principal Component Analysis
Clear separation of treatment and control groups using Principal Component Analysis. (Click to learn more)

Figure 3 Differential microRNA expression in control versus treated animals
Differential microRNA expression in control versus treated animals. (Click to learn more)

Figure 4 Fold increase of rno-miR-21-5p, rno miR-34a-5p and rno-miR-192-5p upon reference nephrotoxin treatment
Increase of microRNAs upon treatment. (Click to learn more)
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Prod. No.
Product Name
Description Notes
203851 Toxicology Focus microRNA PCR Panel, Human, 96 well (V4.RO) miRCURY LNA™ Universal RT microRNA PCR, 4x Ready-to-Use Toxicology Focus microRNA PCR Panels. Each panel comprises one 96-well plate with 87 LNA™ microRNA primer sets focusing on toxicology relevant human microRNAs, reference candidates and spike-ins. For use with Roche LightCycler 480 instruments. Use with ExiLENT master mix only
203852 Toxicology Focus microRNA PCR Panel, Human, 96 well (V4.AF) miRCURY LNA™ Universal RT microRNA PCR, 4x Ready-to-Use Toxicology Focus microRNA PCR Panels. Each panel comprises one 96-well plate with 87 LNA™ microRNA primer sets focusing on toxicology relevant human microRNAs, reference candidates and spike-ins. For use with ABI Fast instrument series and ABI StepOne Plus. Use with ExiLENT master mix only
203853 Toxicology Focus microRNA PCR Panel, Human, 96 well (V4.MI) miRCURY LNA™ Universal RT microRNA PCR, 4x Ready-to-Use Toxicology Focus microRNA PCR Panels. Each panel comprises one 96-well plate with 87 LNA™ microRNA primer sets focusing on toxicology relevant human microRNAs, reference candidates and spike-ins. For use with BioRad iQ series, Eppendorf, Stratagene Mx4000 and AB 7000 series instruments*. Use with ExiLENT master mix only
203854 Toxicology Focus microRNA PCR Panel, Human, 96 well (V4.ST) miRCURY LNA™ Universal RT microRNA PCR, 4x Ready-to-Use Toxicology Focus microRNA PCR Panels. Each panel comprises one 96-well plate with 87 LNA™ microRNA primer sets focusing on toxicology relevant human microRNAs, reference candidates and spike-ins. For use with Stratagene Mx3000P series. Use with ExiLENT master mix only
203855 Toxicology Focus microRNA PCR Panel, Human, 384 well (V4.R) miRCURY LNA™ Universal RT microRNA PCR, 8x Ready-to-Use Toxicology Focus microRNA PCR Panels supplied in two 384-well PCR plates. Each panel comprises 87 LNA™ microRNA primer sets focusing on toxicology relevant human microRNAs, reference candidates and spike-ins. For use with Roche LightCycler 480 instruments. Use with ExiLENT master mix only
203856 Toxicology Focus microRNA PCR Panel, Human, 384 well (V4.M) miRCURY LNA™ Universal RT microRNA PCR, 8x Ready-to-Use Toxicology Focus microRNA PCR Panels supplied in two 384-well PCR plates. Each panel comprises 87 LNA™ microRNA primer sets focusing on toxicology relevant human microRNAs, reference candidates and spike-ins. For use with ABI 7900HT and other instruments. Use with ExiLENT master mix only
203857 Toxicology Focus microRNA PCR Panel, Rat, 96 well (V4.RO) miRCURY LNA™ Universal RT microRNA PCR, 4x Ready-to-Use Toxicology Focus microRNA PCR Panels. Each panel comprises one 96-well plate with 87 LNA™ microRNA primer sets focusing on toxicology relevant rat microRNAs, reference candidates and spike-ins. For use with Roche LightCycler 480 instruments. Use with ExiLENT master mix only
203858 Toxicology Focus microRNA PCR Panel, Rat, 96 well (V4.AF) miRCURY LNA™ Universal RT microRNA PCR, 4x Ready-to-Use Toxicology Focus microRNA PCR Panels. Each panel comprises one 96-well plate with 87 LNA™ microRNA primer sets focusing on toxicology relevant rat microRNAs, reference candidates and spike-ins. For use with ABI Fast instrument series and ABI StepOne Plus. Use with ExiLENT master mix only
203859 Toxicology Focus microRNA PCR Panel, Rat, 96 well (V4.MI) miRCURY LNA™ Universal RT microRNA PCR, 4x Ready-to-Use Toxicology Focus microRNA PCR Panels. Each panel comprises one 96-well plate with 87 LNA™ microRNA primer sets focusing on toxicology relevant rat microRNAs, reference candidates and spike-ins. For use with BioRad iQ series, Eppendorf, Stratagene Mx4000 and AB 7000 series instruments*. Use with ExiLENT master mix only
203860 Toxicology Focus microRNA PCR Panel, Rat, 96 well (V4.ST) miRCURY LNA™ Universal RT microRNA PCR, 4x Ready-to-Use Toxicology Focus microRNA PCR Panels. Each panel comprises one 96-well plate with 87 LNA™ microRNA primer sets focusing on toxicology relevant rat microRNAs, reference candidates and spike-ins. For use with Stratagene Mx3000P series. Use with ExiLENT master mix only
203861 Toxicology Focus microRNA PCR Panel, Rat, 384 well (V4.R) miRCURY LNA™ Universal RT microRNA PCR, 8x Ready-to-Use Toxicology Focus microRNA PCR Panels supplied in two 384-well PCR plates. Each panel comprises 87 LNA™ microRNA primer sets focusing on toxicology relevant rat microRNAs, reference candidates and spike-ins. For use with Roche LightCycler 480 instruments. Use with ExiLENT master mix only
203862 Toxicology Focus microRNA PCR Panel, Rat, 384 well (V4.M) miRCURY LNA™ Universal RT microRNA PCR, 8x Ready-to-Use Toxicology Focus microRNA PCR Panels supplied in two 384-well PCR plates. Each panel comprises 87 LNA™ microRNA primer sets focusing on toxicology relevant rat microRNAs, reference candidates and spike-ins. For use with ABI 7900HT and other instruments. Use with ExiLENT master mix only


NOTICE TO PURCHASER: LIMITED LICENSE Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,994,056 and 6,171,785. The purchase of this product includes a limited, nontransferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
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