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Pick-&-Mix microRNA PCR Panels

Design your own 96- and 384-well qPCR plates. Drag and drop miRCURY LNA™ Universal RT microRNA PCR assays or any custom microRNA, mRNA or lncRNA assay onto the plates using our easy-to-use online design tool. Pick-&-Mix the assays most relevant for your project.


  • High-quality custom designed microRNA qPCR plates available in 96 and 384-well format
  • Highly flexible – Customize to favored plate type, layout, PCR instrument and batch size
  • Superior microRNA PCR system – Choose primer sets from Exiqon’s vast collection of thoroughly validated sensitive miRCURY LNA™ Universal RT microRNA PCR assays
  • Include your own primer sets - use Exiqon’s custom design tools for microRNA or mRNA/lncRNA to target novel microRNAs or validate NGS data
  • Combine microRNA and mRNA detection in the same PCR plate
  • Save sample and time – Focus only on your microRNAs of interest
  • Convenient – Ideal for microRNA profiling on a medium to large number of samples
  • Exceptional accuracy – offers the sensitivity needed for reliable microRNA detection and profiling from difficult samples including FFPE, LCM, serum/plasma and urine
  • Fast and easy – plates are delivered within a few days in ready-to-use PCR plates

Pick-&-Mix – Design your own microRNA qPCR panels

Exiqon’s miRCURY LNA™ Universal RT microRNA PCR system offers unmatched sensitivity and specificity coupled with the convenience of a single universal reverse transcription step. Profile hundreds of microRNAs on panels using just 20ng total RNA without the need of pre-amplification. The whole process takes just 3 hours (Figure 1).

In addition to our predesigned panels, Exiqon offers custom Pick-&-Mix microRNA PCR Panels (Figure 2). Design your own 96- or 384-well microRNA qPCR plates based on a fully flexible layout and several conveniently pre-defined layouts. Place miRCURY LNA™ Universal RT microRNA PCR assays or your own custom LNA™-enhanced assays onto the panel using an intuitive and easy-to-use online plate configurator. The panels are delivered ready-to-use containing one single assay per well for a 10 μL reaction volume - just add cDNA and ExiLENT SYBR® Green master mix (Figure 3).


Coverage

Custom Pick-&-Mix microRNA PCR Panels are available in 2 different formats:
  • 96 well Ready-to-Use . Design your panels from one of three available layouts ( click to view layouts ). Choose from our vast selection of experimentally validated LNA™ microRNA PCR primer sets and reference gene primer sets for human, mouse and rat microRNA quantification
  • 384 well Ready-to-Use . Design your panels from one of four available layouts ( click to view layouts ). Choose from our vast selection of experimentally validated LNA™ microRNA PCR primer sets and reference gene primer sets for human, mouse and rat microRNA quantification
96 well PCR plate layouts
10 miRNAs x 8 samples
22 miRNAs x 4 samples
92 miRNAs x 1 sample

View plate layouts
384 well PCR plate layouts
22 miRNAs x 16 samples
46 miRNAs x 8 samples
94 miRNAs x 4 samples
380 miRNAs x 1 sample
View plate layouts


The LNA™ microRNA PCR primer sets are identical to those available in Exiqon’s other ready-to-use panels making it easy to shift between the different product types and still get the same reliable qPCR results. Choose between >940 human/viral and >750 mouse and rat microRNA assays. Since many of these microRNAs are also present in other organisms, it is possible to design plates for these organisms as well. For a list of available assays, please refer to the layout files of the microRNA Ready-to-use PCR Panels . Three human (SNORD38B, SNORD49A, U6 snRNA) and three mouse and rat (RNU1A1, RNU5G, U6 snRNA) reference primer sets are available.

Pick-&-Mix microRNA PCR Panels come with a pre-positioned interplate calibrator (IPC) in replicates allowing optimal calibration and control assessment in multi-plate studies. The IPC is mandatory, but can be re-positioned to suit your preferred plate layout. In additional, the panel is displayed with pre-positioned one or more UnISp6 RNA Spike-in controls (CP). RNA spike-in controls may be used for monitoring the cDNA synthesis or RNA purification. The UniSp6 CP assay can be removed and substituted by microRNA primer set(s) or other RNA spike-in control assays. Alternative RNA spike-in controls include UniSp2, UniSp4, UniSp5 and cel-miR-39-3p.


Add custom assays

It is now possible to add custom assays to your Pick-&-Mix plate. Custom assays are designed through one of our custom assay design tools:
  • Custom microRNA / small RNA assays . Design LNA™-enhanced PCR primers for sequences not available among our pre-designed assays, e.g., novel microRNAs identified using Next Generation Sequencing.
  • Custom mRNA / lncRNA assays . Detect long and short RNAs on the same PCR plate. This option requires the addition of a Random RT primer in the cDNA reaction (Prod no 309080).



How to order

  • View the product list at the bottom of this page . 96-well and 384-well plates are available. Panels can be ordered in packs of 8. Order additional plates in increments of 4.
  • Click on the “Configure your plates” button at the top of this page . In the online configuration tool you will be asked to provide the following information: plate type, instrument, plate name, user email (plate layout file will be sent by email), plate layout, microRNA assay information and order size. For saving and keeping track of your panel configuration, we recommend that you create an account and sign in.
  • Login is required if you wish to add custom assays to the plate , as this option needs access to your personal custom designs.
  • Your final GenEx-compatible plate-layout file can be downloaded from the plate configurator . This file is also sent to the email address you provided. If you were signed in during the design, the plate layout is available from your Exiqon account.
  • You can order the product directly from our website . Alternatively, press the “Download order as pdf” button from within your shopping basket. This pdf can be used to order the product at a later date, through Exiqon or a distributor / purchaser.

A flexible solution for short and long RNA profiling

Pick-&-Mix microRNA PCR Panels offer great flexibility. Choose between a wide range of plate types, plate layout and batch sizes. Select which assays to include and where they are positioned on the plate. The plates are compatible with most Real-time PCR instruments (Figure 4). The high performance of the miRCURY LNA™ Universal RT microRNA PCR system means that the system is also very flexible in terms of sample type. For individual assays, accurate quantification can be achieved using just 1 pg total RNA which means that the system is ideal for difficult samples, such as FFPE, LCM and serum/plasma.


Plates to fit your project

The high flexibility of the Pick-&-Mix microRNA PCR Panels means that the product can be tailored to fit your needs. It is ideal for investigating microRNA signatures on a medium to large number of samples. Use Pick-&-Mix microRNA PCR Panels to validate your microarray or PCR panel screenings. Design your own disease or pathway-specific qPCR plates or use the plates for biomarker discovery or validation in a large number of samples. Save time and money by focusing your microRNA profiling experiment on the microRNAs most relevant for your project.


Easy data analysis

Pick-&-Mix microRNA PCR Panels are fully compatible with Exiqon’s GenEx qPCR analysis software. The plate layout file is designed for direct import into GenEx for easy data analysis. It offers user-friendly step-by-step guides for analysis of your data. For more information and to download a free trial, please visit exiqon.com/mirna-pcr-analysis .



Figure 1 miRCURY LNA™ Universal RT PCR workflow
Overview of the miRCURY LNA™ Universal RT PCR workflow. (Click to learn more)



Figure 2 Overview of the available content and instrument compatibility for the Pick-&-Mix microRNA PCR Panels.
Overview of Pick-&-Mix microRNA PCR Panels. (Click to learn more)


Figure 3 Fast and easy plate handling
Fast and easy plate handling. (Click to learn more)

Figure 4
Instrument coverage of the panels. (Click to learn more)
View experimental data on microRNA Ready-to-Use PCR panels
Products ready to order
 
Prod. No. Product Name Description Price
203818 Pick-&-Mix microRNA PCR Panel, 384 well Ready-to-Use ( R) Pick-&-Mix microRNA PCR panel, 384 well Ready-to-Use (R) miRCURY LNA™ Universal RT microRNA PCR panels with custom selection of LNA™ microRNA PCR primer sets First 8 plates. USD 4480.39
Pick-&-Mix microRNA PCR Panel, 384 well Ready-to-Use ( R) First 12 plates. USD 6160.95
Pick-&-Mix microRNA PCR Panel, 384 well Ready-to-Use ( R) Per additional 4 plates. USD 1152.20
203819 Pick-&-Mix microRNA PCR Panel, 384 well Ready-to-Use (M) Pick-&-Mix microRNA PCR panel, 384 well Ready-to-Use (M) miRCURY LNA™ Universal RT microRNA PCR panels with custom selection of LNA™ microRNA PCR primer sets First 8 plates. USD 4480.39
Pick-&-Mix microRNA PCR Panel, 384 well Ready-to-Use (M) First 12 plates. USD 6160.95
Pick-&-Mix microRNA PCR Panel, 384 well Ready-to-Use (M) Per additional 4 plates. USD 1152.20
203893 Pick-&-Mix microRNA PCR Panel, 96 well Ready-to-Use (RO) Pick-&-Mix microRNA PCR panel, 96 well Ready-to-Use (RO) miRCURY LNA™ Universal RT microRNA PCR panels with custom selection of LNA™ microRNA PCR primer sets First 8 plates. USD 1678.38
Pick-&-Mix microRNA PCR Panel, 96 well Ready-to-Use (RO) First 12 plates. USD 2239.65
Pick-&-Mix microRNA PCR Panel, 96 well Ready-to-Use (RO) Per additional 4 plates. USD 690.87
203894 Pick-&-Mix microRNA PCR Panel, 96 well Ready-to-Use (AF) Pick-&-Mix microRNA PCR panel, 96 well Ready-to-Use (AF) miRCURY LNA™ Universal RT microRNA PCR panels with custom selection of LNA™ microRNA PCR primer sets First 8 plates. USD 1678.38
Pick-&-Mix microRNA PCR Panel, 96 well Ready-to-Use (AF) First 12 plates. USD 2239.65
Pick-&-Mix microRNA PCR Panel, 96 well Ready-to-Use (AF) Per additional 4 plates. USD 690.87
203895 Pick-&-Mix microRNA PCR Panel, 96 well Ready-to-Use (MI) Pick-&-Mix microRNA PCR panel, 96 well Ready-to-Use (MI) miRCURY LNA™ Universal RT microRNA PCR panels with custom selection of LNA™ microRNA PCR primer sets First 8 plates. USD 1678.38
Pick-&-Mix microRNA PCR Panel, 96 well Ready-to-Use (MI) First 12 plates. USD 2239.65
Pick-&-Mix microRNA PCR Panel, 96 well Ready-to-Use (MI) Per additional 4 plates. USD 690.87
203896 Pick-&-Mix microRNA PCR Panel, 96 well Ready-to-Use (ST) Pick-&-Mix microRNA PCR panel, 96 well Ready-to-Use (ST) miRCURY LNA™ Universal RT microRNA PCR panels with custom selection of LNA™ microRNA PCR primer sets First 8 plates. USD 1678.38
Pick-&-Mix microRNA PCR Panel, 96 well Ready-to-Use (ST) First 12 plates. USD 2239.65
Pick-&-Mix microRNA PCR Panel, 96 well Ready-to-Use (ST) Per additional 4 plates. USD 690.87
203897 Pick-&-Mix microRNA PCR Panel, 96 well Ready-to-Use (CFX) Pick-&-Mix microRNA PCR panel, 96 well Ready-to-Use (CFX) miRCURY LNA™ Universal RT microRNA PCR panels with custom selection of LNA™ microRNA PCR primer sets First 8 plates. USD 1678.38
Pick-&-Mix microRNA PCR Panel, 96 well Ready-to-Use (CFX) First 12 plates. USD 2239.65
Pick-&-Mix microRNA PCR Panel, 96 well Ready-to-Use (CFX) Per additional 4 plates. USD 690.87
203899 Pick-&-Mix microRNA PCR Panel, 96 well bulk plate for Fluidigm miRCURY LNA™ Universal RT microRNA PCR panels with custom selection of LNA™ microRNA PCR primer sets, 96-well PCR bulk plate for 3 Fluidigm chips USD 3500.00


NOTICE TO PURCHASER: DISCLAIMER OF LICENSE No license is conveyed with the purchase of this product under any of US Patents Nos. 5,210,015, 5,487,972, 5,804,375, 5,994,056, 6,171,785, 6,214,979, 5,538,848, 5,723,591, 5,876,930, 6,030,787, and 6,258,569, and corresponding patents outside the United States, or any other patents or patent applications, relating to the 5’ Nuclease and dsDNA-Binding Dye Processes. For further information contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
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