Exiqon
Home Search Contact Print
0 items in basket0
Sign In
 
 
Products Services Applications Resource Center Ordering About Exiqon
microRNA Research
lncRNA Research
mRNA Research
DNA Research
Custom Oligos
Other Reagents
RNA Isolation
Sequencing
Microarray Analysis
Real-time PCR
Northern Blotting
In Situ Hybridization
Functional Analysis
RNA Preparation
Sequencing
Microarray analysis
Real-time PCR
In Situ Hybridization
Antisense
RNA Preparation
Sequencing
Microarray analysis
Real-time PCR
In Situ Hybridization
Antisense
SNP Detection
Sequencing
Microarray Analysis
In Situ Hybridization
LNA Phosphoramidites
A2 Quencher
RNA Isolation Services
microRNA Array Services
microRNA PCR Services
Sequencing Services
microRNA ISH Services
Custom Pharma Service
Isolation
Microarray Analysis
Northern Blotting
Real-time PCR
In Situ Hybridization
Functional Analysis
SNP Detection
RNA
microRNA
RNA
DNA
microRNA
microRNA
mRNA
microRNA
Other RNAs
DNA
microRNA
RNA
DNA
microRNA Resources
mRNA and lncRNA Resources
Documents
Reading rooms
Technology
Oligo design tools
Movies
microRNA
Research Guide
Reading rooms
Application Stories
Bioinformatics and data analysis
What are lncRNAs?
Application Stories
Bioinformatics
Oligo modifications
LNA™
AQ-link
Order microRNA products
Order custom oligos
Distributors
Global Service Providers
General Information
Contact
News
History
Management
Board of Directors
Newsletter
Career
Sign up to Newsletter
Newsletter Back Issues
 

miRSIGN Colon Cancer Test (RUO)

The miRSIGN Colon Cancer Test (RUO) provides a fast and easy method of determining whether or not colon cancer cells are present in formalin fixed paraffin (FFPE) embedded colon tissue specimens. Four microRNAs linked to colon cancer are profiled using Exiqon’s robust microRNA qPCR system and the resulting signature can be used to categorize the sample as tumor or normal.


NOTE: Although the signature is indicative of malignancy this test is designed for Research-Use-Only and is not to be used in a clinical setting.
  • Fast and easy PCR-based detection of colon cancer cells in FFPE samples
  • Ideal for tumor assessment where histological analyses provide unclear results
  • Based on Exiqon’s robust miRCURY LNA™ Universal PCR microRNA PCR System which offers excellent and reliable results from FFPE samples
  • Cancer-related microRNAs detected with unmatched sensitivity and specificity
  • 98% specificity in distinguishing colon cancer cells from normal cells

Features

The miRSIGN Colon Cancer Test (RUO) is based on the miRCURY LNA™ Universal RT microRNA PCR system and consists of LNA™ primer sets and accessory reagents required to analyze the expression levels of four key microRNAs known to be linked to colon cancer, in formalin-fixed, paraffin-embedded (FFPE) colon carcinoma tissue. This 4-miR signature provides a combined numerical expression level that is compared to a normal reference range and can be used to categorize the tissue sample as tumor or normal.

Content

The Kit contains all reagents required for performing the miRSIGN Colon Cancer Test (RUO) using total RNA extracted from FFPE tissue samples. The kit contains reagents sufficient for testing 100 samples (500 reactions):

Universal cDNA Synthesis Kit II, 8-64 rxns (2 kits)
ExiLENT SYBR® Green master mix, 2.5ml, 500 rxns (1 kit)
hsa-miR-21-5p LNA™ PCR primer set, UniRT
hsa-miR-34a-5p LNA™ PCR primer set, UniRT
hsa-miR-126-3p LNA™ PCR primer set, UniRT
hsa-miR-143-3p LNA™ PCR primer set, UniRT

The test is developed for RNA extracted from FFPE tissue blocks with a tissue area of > 4 mm 2 or one unstained 10 µm slide.

Application

Recent research has shown that microRNAs may be responsible for the regulation of up to 40% of all oncogene expression. Because of their involvement in pathway regulation and other biological functions , microRNAs show great potential for use as diagnostic markers. Furthermore, clinical studies have found associations between microRNA expression patterns and treatment outcomes for several cancers, including colon cancer. The miRSIGN Colon Cancer Test (RUO) is highly reproducible (see experimental data) and based on the most sensitive and specific microRNA qPCR system available according to the miRQC study published in Nature Methods.

Read more about the miRCURY LNA™ Universal RT microRNA PCR system.


About the miRSIGN Colon Cancer Test (RUO)

The test detects the expression level of four microRNAs (hsa-miR-21-5p in combination with three other human microRNAs) which provides a numerical combined expression level that can be compared to a normal reference range and used to categorize the tissue specimens.

Internal validation studies have shown very high specificity of the test in distinguishing colon cancer specimens versus normal cancer specimens (98% specific in distinguishing colon cancer cells versus normal cells) (Figure 1).

The miRSIGN Colon Cancer Test (RUO) is based on Exiqon’s miRCURY LNA™ Universal RT microRNA PCR system which combines a universal RT reaction with microRNA specific LNA™-enhanced primers enabling unmatched sensitivity and microRNA quantification (Figure 2a). By using the same RT reaction as template in all subsequent PCR reactions, the number of pipetting steps is reduced to a minimum thereby minimizing the technical variation achieving an extremely reproducible system even from site-to-site (Figure 2b). These features in combination have resulted in the miRSIGN Colon Cancer Test (RUO) – a very robust test with a high level of accuracy and reproducibility (Figure 3).
Figure 1 miRSIGN Colon Cancer Assay (RUO)
miRSIGN Colon Cancer Assay (RUO) Application (Click to learn more)

Figure 2a microRNA copies in PCR
microRNA copies in PCR (Click to learn more)

Figure 2b microRNA copies in PCR
High reproducibility of the four microRNA signature assays in the miRSIGN Colon Cancer Test (RUO) (Click to learn more)

Figure 3 Excellent run-to-run reproducibility
Excellent run-to-run reproducibility (Click to learn more)

Products ready to order Click to view pricing
Showing 1 - 1 of 1 products
Page:
1
Prod. No.
Product Name
Description Notes
  Privacy   Sitemap   Legal