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miRCURY LNA™ Universal RT microRNA PCR Starter Kit

The miRCURY LNA™ Universal microRNA PCR Starter Kit contains all reagents required to perform 20 cDNA reactions and 100 PCR reactions. The kit includes a spike-in control primer set (UniSp6), one candidate endogenous control primer set (miR-103a-3p) and two validated primer sets of your choice. This will enable quantification of up to 20 samples with a minimum of 4 assays per sample.


Order in 3 simple steps

  • Click on the 'Order now' button below
  • Select your primer sets from the drop-down menu and press the 'Add to basket' button
  • Click 'View basket' and proceed with the check-out
  • Cost effective introduction to the most sensitive microRNA qPCR system available
  • No false positives, just true microRNA signals (see specificity results)
  • Contains everything you need to perform small scale experiments
  • Includes RNA spike-in template and Control Primer set for monitoring performance
  • Fast and very easy protocol
  • Experience the superior performance in your own hands
The miRCURY LNA™ Universal microRNA PCR Starter Kit contains all reagents required to perform 20 cDNA reactions and 100 PCR reactions. The kit includes a spike-in control primer set (UniSp6), one candidate endogenous control primer set (miR-103a-3p) and two validated primer sets of your choice. This will enable quantification of up to 20 samples with a minimum of 4 assays per sample. In addition, the kit contains the following reagents:

  • Universal cDNA Synthesis. All the reagents needed for fast and convenient microRNA polyadenylation and reverse transcription in a single reaction step. By using a single universal RT reaction as template in all subsequent PCR reactions, the procedure is greatly simplified compared to systems that require microRNA-specific first-strand synthesis (see figure below).


  • ExiLENT SYBR® Green master mix. A high-performance PCR master mix kit specifically designed for Exiqon's miRCURY LNA™ Universal RT microRNA PCR system.



Contents of the kit

  • cDNA Enzyme, 10 x concentrated, 20 rxn
  • cDNA buffer, 5 x concentrated, 128 μL
  • ExiLENT SYBR® Green master mix, 2 x concentrated, 500μL
  • UniSp6 spike-in RNA, lyophilized, 12 fmol
  • UniSp6 control primer set, lyophilized, 200 rxn
  • hsa-miR-103a-3p (also works for mouse & rat) , lyophilized, 200 rxn
  • 2 additional primer sets (chosen from stock list), lyophilized, 200 rxn
  • RNase-free water, 1,25mL

See more details about the miRCURY LNA™ microRNA PCR System

A unique system developed specifically for microRNA profiling

The miRCURY LNA™ Universal RT microRNA PCR System offers the best available combination of performance and ease-of-use on the market because it unites two important features (Figure 2):
  • One cDNA reaction for all microRNAs – One single Universal first strand cDNA synthesis reaction is used as template, regardless of the number of microRNAs being profiled. This saves precious sample, reduces technical variation, means less pipetting and saves time in the laboratory.
  • Two LNA™-enhanced microRNA qPCR primers – Both qPCR primers are microRNA-specific and optimized with LNA™. LNA™ primers bind with high affinity, and are shorter than standard PCR primers, so that both primers can fit on the microRNA without overlapping. The result is unrivalled sensitivity and specificity, and extremely low background.

Unmatched sensitivity

Universal RT combined with LNA™-enhanced and Tm normalized primers enables accurate and reliable quantification of individual microRNAs from as little as 1 pg total RNA (Figure 3). In comparison to other microRNA real-time PCR systems using either stem-loop or standard DNA primers, the LNA™-enhanced primers offer significantly increased sensitivity, especially for AU-rich microRNAs.

Exceptional sensitivity as well as extremely low background enables accurate quantification of very low levels of microRNA without the need for pre-amplification. This makes the miRCURY LNA™ Universal RT microRNA PCR System suitable for all sample types, and especially samples with low RNA content e.g. biofluids such as serum/plasma.

Fully validated and optimized

All miRCURY LNA™ Universal RT microRNA PCR primer sets have been optimized for maximum sensitivity and thoroughly validated either by wet lab testing or in silico. In wet lab validation, over 95% of assays detect 10 microRNA copies or less in the PCR reaction (Figure 4). The primer sets have also been validated for specific amplification of the target and for minimal background signal.

Truly specific – no false positives

The incorporation of LNA™ in the qPCR primers facilitates the design of assays that can distinguish between microRNA sequences that differ by a single nucleotide. This makes it a truly specific microRNA qPCR platform – the only microRNA platform that gives no false positive signals (Figure 5). In addition, the assays can discriminate between mature microRNA sequences and precursor microRNA (Table 1).

Fast, easy and reproducible

Save time and effort in the laboratory with the 3 hour easy-to-follow protocol that minimizes pipetting. By using the same cDNA as template in all subsequent PCR reactions, the procedure is greatly simplified compared to systems that require microRNA-specific cDNA synthesis.

With fewer pipetting steps, technical variation is also reduced. As a result, it is possible to achieve extremely high reproducibility from day-to-day (Figure 6) and even site-to-site.

Flexible qPCR system

Tailor your experimental setup to your specific need using individual assays or fully flexible custom Pick-&-Mix panels . Using the same cDNA reaction, shift seamlessly between individual primers, pre-designed miRNome and Focus panels, or custom Pick-&-Mix panels.
Figure 1 Overview of the miRCURY LNA™ Universal RT PCR workflow
Overview of the miRCURY LNA™ Universal RT PCR workflow. (Click to learn more)

Figure 2 Schematic outline of the miRCURY LNA™ Universal RT microRNA PCR System
Schematic outline of the miRCURY LNA™ Universal RT microRNA PCR System. (Click to learn more)

Figure 3 Accurate quantitation from down to 1 pg total RNA starting material
Accurate quantitation from down to 1 pg total RNA starting material. (Click to learn more)

Figure 4 Serial dilution of hsa-miR-181a
Serial dilution of hsa-miR-181a. (Click to learn more)

Figure 5 Specificity results from the miRQC Study
Specificity results from the miRQC Study. (Click to learn more)

Table 1 Discrimination between mature and precursor microRNAs
Discrimination between mature and precursor microRNAs. (Click to learn more)

Figure 6 Excellent day-to-day reproducibility
Excellent day-to-day reproducibility. (Click to learn more)

NOTICE TO PURCHASER: DISCLAIMER OF LICENSE No license is conveyed with the purchase of this product under any of US Patents Nos. 5,210,015, 5,487,972, 5,804,375, 5,994,056, 6,171,785, 6,214,979, 5,538,848, 5,723,591, 5,876,930, 6,030,787, and 6,258,569, and corresponding patents outside the United States, or any other patents or patent applications, relating to the 5' Nuclease and dsDNA-Binding Dye Processes. For further information contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
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