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miRCURY LNA™ Universal RT microRNA PCR Starter Kit



Key benefits

  • Cost effective introduction to the most sensitive microRNA qPCR system available
  • No false positives, just true microRNA signals (see specificity results)
  • Contains everything you need to perform small scale experiments
  • Includes RNA spike-in template and Control Primer set for monitoring performance
  • Fast and very easy protocol
  • Experience the superior performance in your own hands

Product description

The miRCURY LNA™ Universal microRNA PCR Starter Kit contains all reagents required to perform 20 cDNA reactions and 100 PCR reactions. The kit includes a spike-in control primer set (UniSp6), one candidate endogenous control primer set (miR-103a-3p) and two validated primer sets of your choice. This will enable quantification of up to 20 samples with a minimum of 4 assays per sample. In addition, the kit contains the following reagents:

  • Universal cDNA Synthesis. All the reagents needed for fast and convenient microRNA polyadenylation and reverse transcription in a single reaction step. By using a single universal RT reaction as template in all subsequent PCR reactions, the procedure is greatly simplified compared to systems that require microRNA-specific first-strand synthesis (see figure below).
  • ExiLENT SYBR® Green master mix. A high-performance PCR master mix kit specifically designed for Exiqon’s miRCURY LNA™ Universal RT microRNA PCR system.





Contents of the kit

  • cDNA Enzyme, 10 x concentrated, 20 rxn
  • cDNA buffer, 5 x concentrated, 128 µL
  • ExiLENT SYBR® Green master mix, 2 x concentrated, 500µL
  • UniSp6 spike-in RNA, lyophilized, 12 fmol
  • UniSp6 control primer set, lyophilized, 200 rxn
  • hsa-miR-103a-3p (also works for mouse & rat) , lyophilized, 200 rxn
  • 2 additional primer sets (chosen from stock list), lyophilized, 200 rxn
  • RNase-free water, 1,25mL

See more details about the miRCURY LNA™ microRNA PCR System



NOTICE TO PURCHASER: DISCLAIMER OF LICENSE No license is conveyed with the purchase of this product under any of US Patents Nos. 5,210,015, 5,487,972, 5,804,375, 5,994,056, 6,171,785, 6,214,979, 5,538,848, 5,723,591, 5,876,930, 6,030,787, and 6,258,569, and corresponding patents outside the United States, or any other patents or patent applications, relating to the 5’ Nuclease and dsDNA-Binding Dye Processes. For further information contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Order in 3 simple steps

  • Click on the 'Order now' button below
  • Select your primer sets from the drop-down menu and press the 'Add to basket' button
  • Click 'View basket' and proceed with the check-out
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