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Exosome Focus microRNA PCR Panels

PCR panels containing miRCURY LNA™ Universal RT microRNA PCR assays for microRNAs typically found in exosomes. Panels are available in ready-to-use 96 and 384 well plates – just add cDNA and PCR master mix.

  • All typically expressed microRNAs found in urine or cerebrospinal fluid samples in one PCR Panel
  • A highly flexible solution that allows you to focus on the microRNAs you need in your exosome project
  • Intuitive plate designer allows replacement of assays in the plates before ordering
  • Sensitivity and specificity for accurate microRNA profiling
  • Fast and easy - ready-to-use format for direct sample application with a full profile in less than 3 hours
  • High compatibility – 96 and 384 well formats compatible with most real-time PCR instruments

Why study exosomes?

Exosomes are cell derived membranous particles ranging in size from 20 to 120 nm, approximately the same size as viruses but considerably smaller than microvesicles (Figure 1). Exosomes are excreted from cells into the surrounding media and can be found in many if not all body fluids. Their proposed role as intercellular hormone-like messengers together with their stability as carrier of proteins and RNA make them ideal as biomarkers for a variety of diseases and biological processes (Figure 2).

Exosomes are secreted by most cell types and are formed by the fusion of multivesicular bodies with the plasma membrane. They are believed to be involved in a number of functions, including:
  • Immune regulation (e.g. tumor derived exosomes may help the tumor to evade the immune response)
  • Blood coagulation
  • Cell migration
  • Cell differentiation
  • Cell-to-cell communication
Microvesicles that are larger than exosomes (up to 1 µm) are typically formed by blebbing of the plasma membrane, whereas exosomes are released by exocytosis from multivesicular bodies of the endosome. 


How to isolate exosomes

The two classical ways to isolate exosomes are either by cumbersome sucrose gradient ultra-centrifugation or by isolation using antibodies specific for one of the surface proteins typically found on exosomes. The last method is very specific and might exclude some sub fractions of exosomes. The miRCURY™ Exosome Isolation Kits ensure a very high recovery of exosomes, using a straightforward and fast protocol. If used in conjunction with miRCURY™ RNA Isolation Kits, high quality microRNA can be extracted from exosomes from various biofluids. 

Although not species specific, the protocols are optimized for working with human biofluids. Recommended starting material for different organisms and sample types can be found in the manual.

Figure 1 The structure of an exosome The structure of an exosome. (Click to learn more)

Figure 2 The hypothesis of exosomal shuttling of microRNAs The hypothesis of exosomal shuttling of microRNAs. (Click to learn more)

Product coverage

The miRCURY LNA™ Universal RT microRNA PCR, Exosome Focus microRNA PCR Panels are offered as a pre-defined Pick-&-Mix microRNA PCR Panels. It is pre-defined lists of assays for microRNAs expressed in either exosomes from urine or exosomes from cerebrospinal fluid (CSF). Both assay lists are designed to fit into a single 96-well PCR plate or as 4 replicates in a 384-well PCR plate. 


The Urine Exosome Focus microRNA PCR Panels contains:
  • 87 microRNA LNA™ PCR primer sets targeting human microRNAs expressed in human exosomes from urine.
  • 5 of the microRNA assays can also be used as potential internal control primers: hsa-miR-103-3p, hsa-miR-191-5p, hsa-miR-93-5p, hsa-miR-423-3p and hsa-miR-423-5p.
  • 5 RNA spike-in controls available for selection in the Pick-&-Mix configurator for use as positive controls e.g. for quality control of the RNA purification and the RT reaction.
  • 3 inter-plate calibrators (3 x UniSp3 IPC).
Download a list of all microRNAs included on the panel (MS Excel) 


The CSF Exosome Focus microRNA PCR Panels contains:
  • 87 microRNA LNA™ PCR primer sets targeting human microRNAs expressed in human exosomes from cerebrospinal fluid.
  • 3 of the microRNA assays can also be used as potential internal control primers: hsa-miR-103-3p, hsa-miR-191-5p and hsa-miR-93-5p.
  • 5 RNA spike-in controls available for selection in the Pick-&-Mix configurator for use as positive controls e.g. for quality control of the RNA purification and the RT reaction.
  • 3 inter-plate calibrators (3 x UniSp3 IPC).
Download a list of all microRNAs included on the panel (MS Excel)


A truly flexible solution

Since the panels are offered in the Pick-&-Mix configurator as a pre-defined panels, the panel composition is not static. Assays can be added or removed according to your needs. This highly flexible solution allows you to design the plates the way you want them in 96 or 384 well formats compatible with a wide range of PCR instruments (Figure 1).


How the assays were selected

All microRNA assays in the panels were selected based on a combination of experimental data from in-house studies on exosomes, using our miRCURY LNA™ Universal RT microRNA PCR system and results published in peer-reviewed journals.

The assays are not a complete list, targeting all microRNAs found in exosomes, but they target those microRNAs typically found. If missing some microRNAs isof a concern it is recommended to run a full miRNome study using micrCURY RNA PCR Panel I+II

Figure 1
Instrument coverage of the panels. (Click to learn more)



Experimental data

Experimental data See Figure 1 for an expression profile of microRNAs from exosomes isolated from urine. Exosomes were isolated from 5 mL of urine using miRCURY Exosome Isolation Kit - Cells, urine and CSF (Prod. No 300102), followed by an RNA extraction using miRCURY RNA Isolation Kit - Cell and Plant (Prod. No 300110). The microRNA profile was examined using the Urine Exosome Focus microRNA PCR Panel.

See Figure 2 for an expression profile of microRNAs from exosomes isolated from cerebrospinal fluid. Exosomes were isolated from 1 mL of cerebrospinal fluid using miRCURY Exosome Isolation Kit - Cells, urine and CSF (Prod. No 300102), followed by an RNA extraction using miRCURY RNA Isolation Kit - Cell and Plant (Prod. No 300110). The microRNA profile was examined using the CSF Exosome Focus microRNA PCR Panel.

Figure 1
Urine microRNA profile. (Click to learn more)

Figure 2
CSF microRNA profile (Click to learn more)

How to order Exosome Focus microRNA Panels

Exosome Focus microRNA Panels are ordered using our Pick-&-Mix microRNA PCR Panel plate configurator. Follow the steps below to order your plates.

1. Click on the button below. This will take you to our Pick-&-Mix microRNA PCR Panel plate configurator.


2. Register an account, sign in to your account or continue without signing in.

3. In Step 1 of the configurator, you are asked to choose between 96 and 384 well plates. Press “Next” to continue to the next step.

4. Select your PCR instrument and click “Next”.

5. In step 3 of the configurator, select the Exosome Focus panel of interest. You will be able to modify the selection of assays at a later stage. Press “Next”.

6. Please provide a name for your plate and enter your email address (email address is entered by default if you are signed in). Your email address is needed in order to send you the plate layout electronically. In this step you can also choose between different plate layouts.  For ordering the Focus Panel in a 96-well plate, you will need to select the 96 well – fully flexible layout . For ordering the Focus Panel in a 384-well plate format you can choose between the “384-well – fully flexible” layout or the “94 microRNA x 4 samples” layout.  Press “Next”.

7. In step 5 of the configurator, you are asked to choose which assays to include on the plate. Press the “Assign all pre-defined assays to panel” button to include all assays. Please note, you will need to add the four additional RNA Spike-in controls manually (UniSp2, UniSp4, UniSp5 and cel-miR-39-3p). These are to be added in the four empty wells at the bottom of the panel. You can add or remove assays from the plate. To add an assay, click the check box next to the assay in the list to the left and press the “Upload to Panel” button. To remove individual assays, click on the well in the plate depiction to select it and then click “Delete Selected Assays”.

8. If you did not log in to your account before entering the plate configurator tool, you will be asked to do so now. In step 6 of the configurator, you can save your plate configuration, make corrections or order plates by pressing “Finalize plates”. This will place the plates in your shopping basket. From here, you can either buy the plates online or request a quote from your local sales representative or distributor if you are located in a country in which Exiqon has a distributor.



NOTICE TO PURCHASER: LIMITED LICENSE Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,994,056 and 6,171,785. The purchase of this product includes a limited, nontransferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
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