Since the discovery of extracellular microRNA only a few years ago, they have been detected in a large number of biofluids and it is becoming clear that these small regulating RNAs can be used as informative biomarkers to assess and monitor the physiopathological state of the body.
However, the study of microRNA in biofluids is challenging both due to the target and the samples involved and requires highly optimized reagents and protocols as well as strict attention to experimental procedure.
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Impact of age, BMI and sex on circulating microRNA profile
Researchers at the University of Greifswald in Germany have investigated the association between plasma microRNA profile and age, body mass index and sex. They used Exiqon's miRCURY Isolation Kit – Biofluids and miRCURY LNA™ Universal RT microRNA PCR system. Read the paper (external link)
Reliable Biofluids microRNA Sequencing Services
A complete sample-to-answer microRNA-seq service from the people who pioneered microRNA biomarker discovery in liquid biopsies. We use RNA isolation and library preparation protocols specifically designed for biofluid samples to give optimal sequencing data with high mapping percentages. Learn more
Webinar: microRNA profiling in liquid biopsies by qPCR and NGS
Watch our recent webinar to hear our valuable tips gained from years of experience profiling microRNAs in biofluid samples.
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Top 5 Tips: microRNA NGS in serum/plasma
Exiqon's pioneering work with biofluids has enabled Exiqon Services to develop sample handling recommendations and optimized protocols for NGS in biofluids to ensure reliable results.
Read the Top 5 tips
Genome-wide comparison of NGS and qPCR for biofluids microRNA profiling
We put qPCR and NGS to the test, using microRNA protocols optimized specifically for biofluid samples, to analyze the same liquid biopsy serum samples on both platforms. Find out how many microRNAs were detected by each platform, how the results compare, and why validation is important. Download the TechNote
Top 5 Tips: RNA isolation from biofluids
Identifying RNA biomarkers in biofluids holds great promise but can be challenging. These samples often contain tiny amounts of RNA and are subject to contamination during handling. We have assembled some of our best tips to let you get the most out of your samples. Read the Top 5 tips
New biomarkers in biofluids
- The potential of microRNA as biomarkers
- Challenges and solutions for profiling microRNAs in biofluids
- Our recent progress in discovery and validation of microRNA biomarkers for prostate and colorectal cancer See the presentation
New exosome presentation based on our AACR poster
Learn how to isolate and profile exosome microRNAs from common biofluids. Get valuable tips on how to set up your experiment and see an example of how microRNA profiles from urine can be used to identify the various stages of prostate cancer.
See the presentation
Independent study shows Exiqon’s biofluid kit outperforms competitors
Researchers at The Johns Hopkins University School of Medicine have compared the performance of Exiqon’s miRCURY™ RNA isolation kit – Biofluids to other commercially available RNA isolation methods and found that it offers the best combination of performance and ease-of-use. Read the paper (external link)
New Exosome Isolation Kits
High quality exosome isolation using a fast protocol for scalable isolation of exosmes from various biofluids. Exosome isolation using miRCURY™ Exosome Isolation Kits ensures seamless integration with the miRCURY™ RNA Isolation Kits and miRCURY LNA™ Universal RT microRNA PCR system. Learn more
Exosome Focus microRNA PCR Panels
PCR panels containing miRCURY LNA™ Universal RT microRNA PCR assays for microRNAs typically found in exosomes. Panels are available in ready-to-use 96 and 384 well plates – just add cDNA and PCR master mix.
microRNA profiling from 1µL serum
In order to identify potential microRNA biomarkers for asthma, a mouse model for asthmatic response was used for microRNA profiling. RNA from the equivalent of 1µL serum was enough to profile over 120 mouse microRNAs on the miRCURY LNA™ Serum/Plasma Focus Panels. Read the story
microRNA profiling in urine
This white paper describes the discovery of microRNA biomarkers of kidney damage in urine samples. Learn about: setting up microRNA profiling studies in urine, strategies for normalization and data analysis and how to Identify microRNA-based markers of kidney damage. Read the paper
Introduction to microRNA in biofluids
microRNAs have been detected in many different biofluids. It has also been shown that extracellular or circulating microRNAs can be biomarkers for a variety of disease. Exiqon offers a range of products specifically well suited to the analysis of microRNA in Biofluids. Find out more
Updated microRNA profiling guidelines: New edition available
Biofluid samples are challenging due to their limited RNA content and susceptibility to effects of pre-analytical variables. The guidelines provide important information and tips to ensure successful microRNA profiling in serum/plasma and other biofluids using microRNA qPCR. Download guidelines
Recommendations for sample collection and handling
In order to ensure high quality samples that perform well in downstream analysis, it is important to pay special attention to the way biofluid samples are collected and handled.
Webinar: microRNA in biofluids
Watch the webinar and learn about: the challenges of microRNA profiling in biofluids, the essentials of developing robust biomarkers in serum/plasma and urine and our recent progress in discovery and validation of microRNA biomarkers for disease and organ damage. View the webinar
Small RNA isolation from Biofluids
Exiqon’s miRCURY RNA Isolation kit – Biofluids is the first RNA purification kit on the market to be optimized for biofluids. Find out more about this easy-to-use kit for isolating high quality microRNA from samples with limited RNA content.
Webinar: Detecting Disease in Blood
This webinar featuring Dr. Colin Pritchard from the University if Washington and Dr. Monty Montano from Boston University School of Medicine presents some of the latest advances in blood based miRNA biomarker research.
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Inside story: microRNA profiling in biofluids
Learn about microRNA profiling in biofluid samples from experienced microRNA researchers and their current projects. Get useful hints and advice for your own profiling study.
Read the exciting application stories
The ideal microRNA PCR system for biofluids
Exiqon's highly sensitive qPCR system is ideal for microRNA profiling from biofluids. Choose between individual assays, miRNome panels, Focus panels and custom plates.
View movie: The ideal microRNA PCR system