LNA™ oligonucleotides in general
|
Questions
|
1. What is LNA™? 2. What are the unique properties of LNA™? 3. When should I use LNA™? 4. For which applications can I use Exiqon's LNA™ oligonucleotide probes? 5. Where can I find references citing the use on LNA™ for my research area?? 6. Does Exiqon provide LNA™ probes for microRNA research? 7. Does Exiqon offer assistance in the design of custom made LNA™ oligonucleotides? 8. Will Exiqon synthesize an LNA™ oligonucleotide of my own design? 9. Will I know the design of the LNA™ oligonucleotide if it has been designed at Exiqon? 10. When should I choose 5’ or 3’ labeling? 11. When should I use DIG-labeled, biotin-labeled and fluorescein-labeled LNA™ oligonucleotides? 12. What is an amino-labeled LNA™ oligonucleotide probe? 13. Can I label the LNA™ oligonucleotides myself? 14. How stable are LNA™ oligonucleotides? 15. How do I order LNA™ oligonucleotides? |
Answer
1. What is LNA™?
Locked Nucleic Acid (LNA™) is a conformationally restricted nucleic acid analogue, in which the ribose ring is "locked" with a methylene bridge connecting the 2’-O atom with the 4’-C atom. LNA™ nucleotides containing the six common nucleobases (T, C, G, A, U and mC) are able to form base-pairs with their complementary nucleotides according to the standard Watson-Crick base pairing rules.
Locked Nucleic Acid (LNA™) is a conformationally restricted nucleic acid analogue, in which the ribose ring is "locked" with a methylene bridge connecting the 2’-O atom with the 4’-C atom. LNA™ nucleotides containing the six common nucleobases (T, C, G, A, U and mC) are able to form base-pairs with their complementary nucleotides according to the standard Watson-Crick base pairing rules.