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RNA spike-in kit for quality control of the RNA isolation and cDNA synthesis steps of a miRCURY LNA™ Universal RT microRNA PCR experiment.
Use the RNA spike-in kit for quality control of the RNA isolation and cDNA synthesis steps of a miRCURY LNA™ Universal RT microRNA PCR experiment. It is important for any qPCR experiment to ensure that the quality of the input RNA is sufficiently high for effective amplification. By introducing spike-in RNAs during RNA isolation and cDNA synthesis and comparing the qPCR results to those from endogenous reference genes, important information regarding the quality of the samples can be obtained (Table 1).
Exiqon’s RNA spike-ins (UniSp2, UniSp4, UniSp5, UniSp6) resemble microRNAs in structure but lack close sequence similarities to known microRNAs. In addition, the kit includes a synthetic version of the C. elegans microRNA, cel-miR-39-3p.
In order to take advantage of the RNA Spike-in kit, you will need to purchase the corresponding LNA™ primer sets separately (with the exception of UniSp6 which is included in Exiqon’s SYBR® Green Master Mix). This means that you have the flexibility to use only the spike-ins you need and don’t have to pay extra for primer sets you are not going to use.
The RNA Spike-in Kit is compatible with Exiqon Pick-&-Mix microRNA PCR Panels and individual miRCURY LNA™ Universal RT microRNA PCR assays.
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