Exiqon
Home Search Contact Print
0 items in basket0
Sign In
 
 
Products Services Applications Resource Center Ordering About Exiqon
microRNA Research
lncRNA Research
mRNA Research
DNA Research
Custom Oligos
Other Reagents
RNA Isolation
Sequencing
Microarray Analysis
Real-time PCR
Northern Blotting
In Situ Hybridization
Functional Analysis
RNA Preparation
Sequencing
Microarray analysis
Real-time PCR
In Situ Hybridization
Antisense
RNA Preparation
Sequencing
Microarray analysis
Real-time PCR
In Situ Hybridization
Antisense
SNP Detection
Sequencing
Microarray Analysis
In Situ Hybridization
LNA Phosphoramidites
A2 Quencher
RNA Isolation Services
microRNA PCR Services
Sequencing Services
XploreRNA
microRNA ISH Services
Custom Pharma Service
Isolation
Microarray Analysis
Northern Blotting
Real-time PCR
In Situ Hybridization
Functional Analysis
SNP Detection
RNA
microRNA
RNA
DNA
microRNA
microRNA
RNA
microRNA
RNA
DNA
microRNA
RNA
DNA
microRNA Resources
mRNA and lncRNA Resources
Documents
Reading rooms
Application Stories
Technology
Oligo design tools
Movies
microRNA
Research Guide
Reading rooms
Application Stories
Bioinformatics and data analysis
What are lncRNAs?
Application Stories
Bioinformatics
Oligo modifications
LNA™
AQ-link
Order microRNA products
Order custom oligos
Distributors
Global Service Providers
General Information
Contact
News
History
Management
Board of Directors
Newsletter
Career
Sign up to Newsletter
Newsletter Back Issues
 

Exiqon GenEx qPCR analysis software

Exiqon has worked together with MultiD Analyses to generate a version of the qPCR GenEx analysis software specially adapted for Exiqon's microRNA qPCR platform. Import qPCR data fast and easy using the new Exiqon qPCR plate wizard. Download a 14 day free trial today and use our step-by-step guide to get started with your qPCR data analysis.

  • Comprehensive pre-processing of qPCR data – including inter-plate calibration, outlier detection and efficiency correction
  • Fast and easy qPCR data import using exclusive wizard – Ideal for high throughput analysis
  • Convenient selection of reference genes using GeNorm and Normfinder
  • Straightforward normalization using reference genes or global mean
  • Advanced statistical analysis available at the click of a button
  • Publication ready plots and graphs
  • Detailed manual and online help available from experts
  • Flexible license options - 6 or 12 months as well as perpetual license available
  • Site license available

Comprehensive and easy-to-use qPCR data analysis

Exiqon has partnered with MultiD to provide a software for qPCR data analysis specifically adapted to our miRCURY LNA™ Universal RT microRNA PCR products. Exiqon GenEx combines advanced qPCR data analysis with a user-friendly streamlined workflow. It can be used for any qPCR data analysis, including both relative and absolute quantification experiments.

Exiqon GenEx contains all functionalities you need to analyze and visualize your qPCR data (Figure 1):
  • Direct import of data through the Exiqon qPCR plate import wizard
    • Import hundreds of instrument export files (qPCR data files) within minutes
  • Data pre-processing for optimal normalization and data handling enabling
    • Easy removal of microRNAs not expressed in the study
    • Quality check of the spike-in values across samples
    • Easy sorting of assays
    • Assay-specific cut-off based on negative control samples
    • Normalization to global mean of all assays expressed below a set cut-off
    • Simplified selection of reference genes using classifier
  • Comprehensive statistical analyses
    • Publication-ready heat-maps and PCA plots generated in a just a few simple clicks
See a complete list of features (.pdf)

Easy import of data from Exiqon’s PCR panels

The Exiqon GenEx software offers fast and easy import of data from any miRCURY LNA™ Universal RT microRNA PCR panel. Using the new Exiqon qPCR plate import wizard , instrument files can be imported and merged in minutes. Just activate the wizard and select your Exiqon qPCR panel, e.g., Human microRNA PCR Panel I+II and upload your data file.

In addition, the wizard tool makes designing your PCR experiment and classifying your data fast and straightforward. Exiqon’s qPCR plate import wizard is available exclusively with the Exiqon GenEx software.

Together with our Step-by-step guide to data analysis , GenEx ensures that you get the most out of your qPCR data.

Exiqon GenEx product coverage:

Exiqon GenEx Professional: The complete software for direct instrument export file upload via the Exiqon qPCR plate import wizard, all unique data pre-processing tools and classification methods. It further includes many data presentation tools and simple and advanced statistical analysis functionalities. Enables high-throughput analysis of qPCR data and imports several hundreds of instrument export files within a few minutes. The license is for one PC for perpetual use or time limited depending on product.

Exiqon GenEx Enterprise: Includes all the features and functionalities of the Exiqon GenEx Professional as well as additional advanced statistical analyses and expression profiling analyses. The license is for one PC for perpetual use or time limited depending on product.

View comparison between the versions (.pdf)

A USB license key is available allowing Exiqon GenEx software access from an unlimited number of PCs, one user at the time. Install the software on several computers and connect the USB key to the one you are planning on using with the program. A valid license for GenEx Pro or GenEx Enterprise is required for purchases of USB keys.

Please contact us for information about a server-based site license.

Academic
6 months 12 months Unlimited Site license USB
Pro 207026 207030 207014 207035 207033
Enterprise 207028 207032 207016 207037 207040


Industrial
6 months 12 months Unlimited Site license USB
Pro 207025 207029 207013 207034 207033
Enterprise 207027 207031 207015 207036 207040


Excellent data pre-processing

One of the most important parts of a qPCR experiment is the pre-processing of raw data for subsequent statistical analysis. Pre-processing needs to be performed with consistence, in the correct order and with confidence. GenEx has a streamlined and user-friendly interface which aids data handling.

For accurate and robust normalization of qPCR data, GenEx has the advantage of incorporating both NormFinder and GeNorm in the software. Thus, you get two algorithms on which to base your choice of reference genes in one software installation. Normalization to multiple reference genes or to global mean expression is done by the click of a button.

Comprehensive statistical analyses

GenEx also offers advanced statistical solutions for post-normalization data analysis. Powerful presentation tools facilitate the generation of professional illustrations of even the most complex experimental designs. Current features include parametric and non-parametric statistical tests, clustering methods, principal component analysis, artificial neural networks, and much more. View a complete list of features.

Figure 1 Exiqon GenEx workflow.
Exiqon GenEx workflow. (Click to learn more)


Figure 2 Easy import of qPCR data
Easy import of qPCR data. (Click to learn more)


System requirements for GenEx:

  • Platforms Supported *: Windows® Xp/Vista/7
  • Minimum: 700 MHz AMD® /Intel® CPU, 512 MB RAM
  • Recommended: 1600 MHz or faster AMD® /Intel® CPU, 1024 or more MB RAM
* Use of GenEx on Mac OS X requires a Windows Emulator

Get started with the Exiqon GenEx software

In order for you to experience the power and convenience of qPCR data analysis using GenEx, we offer a free 14 day trial of the software.

Click on the link below to be taken to a download page.


To purchase a license for Exiqon GenEx, please use the webshop and select the license type you require.

Once you confirm your order, you will receive a license key by email within 5 business days.

A unique system developed specifically for microRNA profiling

The miRCURY LNA™ Universal RT microRNA PCR System offers the best available combination of performance and ease-of-use on the market because it unites two important features (Figure 2):
  • One cDNA reaction for all microRNAs – One single Universal first strand cDNA synthesis reaction is used as template, regardless of the number of microRNAs being profiled. This saves precious sample, reduces technical variation, means less pipetting and saves time in the laboratory.
  • Two LNA™-enhanced microRNA qPCR primers – Both qPCR primers are microRNA-specific and optimized with LNA™. LNA™ primers bind with high affinity, and are shorter than standard PCR primers, so that both primers can fit on the microRNA without overlapping. The result is unrivalled sensitivity and specificity, and extremely low background.

Unmatched sensitivity

Universal RT combined with LNA™-enhanced and Tm normalized primers enables accurate and reliable quantification of individual microRNAs from as little as 1 pg total RNA (Figure 3). In comparison to other microRNA real-time PCR systems using either stem-loop or standard DNA primers, the LNA™-enhanced primers offer significantly increased sensitivity, especially for AU-rich microRNAs.

Exceptional sensitivity as well as extremely low background enables accurate quantification of very low levels of microRNA without the need for pre-amplification. This makes the miRCURY LNA™ Universal RT microRNA PCR System suitable for all sample types, and especially samples with low RNA content e.g. biofluids such as serum/plasma.

Fully validated and optimized

All miRCURY LNA™ Universal RT microRNA PCR primer sets have been optimized for maximum sensitivity and thoroughly validated either by wet lab testing or in silico. In wet lab validation, over 95% of assays detect 10 microRNA copies or less in the PCR reaction (Figure 4). The primer sets have also been validated for specific amplification of the target and for minimal background signal.

Truly specific – no false positives

The incorporation of LNA™ in the qPCR primers facilitates the design of assays that can distinguish between microRNA sequences that differ by a single nucleotide. This makes it a truly specific microRNA qPCR platform – the only microRNA platform that gives no false positive signals (Figure 5). In addition, the assays can discriminate between mature microRNA sequences and precursor microRNA (Table 1).

Fast, easy and reproducible

Save time and effort in the laboratory with the 3 hour easy-to-follow protocol that minimizes pipetting. By using the same cDNA as template in all subsequent PCR reactions, the procedure is greatly simplified compared to systems that require microRNA-specific cDNA synthesis.

With fewer pipetting steps, technical variation is also reduced. As a result, it is possible to achieve extremely high reproducibility from day-to-day (Figure 6) and even site-to-site.

Flexible qPCR system

Tailor your experimental setup to your specific need using individual assays or fully flexible custom Pick-&-Mix panels . Using the same cDNA reaction, shift seamlessly between individual primers, pre-designed miRNome and Focus panels, or custom Pick-&-Mix panels.
Figure 1 Overview of the miRCURY LNA™ Universal RT PCR workflow
Overview of the miRCURY LNA™ Universal RT PCR workflow. (Click to learn more)

Figure 2 Schematic outline of the miRCURY LNA™ Universal RT microRNA PCR System
Schematic outline of the miRCURY LNA™ Universal RT microRNA PCR System. (Click to learn more)

Figure 3 Accurate quantitation from down to 1 pg total RNA starting material
Accurate quantitation from down to 1 pg total RNA starting material. (Click to learn more)

Figure 4 Serial dilution of hsa-miR-181a
Serial dilution of hsa-miR-181a. (Click to learn more)

Figure 5 Specificity results from the miRQC Study
Specificity results from the miRQC Study. (Click to learn more)

Table 1 Discrimination between mature and precursor microRNAs
Discrimination between mature and precursor microRNAs. (Click to learn more)

Figure 6 Excellent day-to-day reproducibility
Excellent day-to-day reproducibility. (Click to learn more)

Products ready to order Click to view pricing
Showing 1 - 20 of 27 products
Page:
1
Prod. No.
Product Name
Description Notes
207009 Exiqon GenEx wizard Exiqon qPCR plate import wizard addition to GenEx, for current GenEx customers
207013 Exiqon GenEx 6 Pro, Industrial GenEx 6 Pro with Exiqon qPCR wizard, qPCR analysis software, perpetual license for industrial use
207014 Exiqon GenEx 6 Pro, Academic GenEx 6 Pro with Exiqon qPCR wizard, qPCR analysis software, perpetual license for academic use
207015 Exiqon GenEx 6 Enterprise, Industrial GenEx 6 Enterprise with Exiqon qPCR wizard, qPCR analysis software, perpetual license for industrial use
207016 Exiqon GenEx 6 Enterprise, Academic GenEx 6 Enterprise with Exiqon qPCR wizard, qPCR analysis software, perpetual license for academic use
207018 Exiqon GenEx 6 Pro, Academic, 1 month GenEx 6 Pro with Exiqon qPCR wizard, qPCR analysis software, 1 month license for academic use
207022 Exiqon GenEx 6 Pro, Academic, 3 month GenEx 6 Pro with Exiqon qPCR wizard, qPCR analysis software, 3 month license for academic use
207025 Exiqon GenEx 6 Pro, Industrial, 6 month GenEx 6 Pro with Exiqon qPCR wizard, qPCR analysis software, 6 month license for industrial use
207026 Exiqon GenEx 6 Pro, Academic, 6 month GenEx 6 Pro with Exiqon qPCR wizard, qPCR analysis software, 6 month license for academic use
207027 Exiqon GenEx 6 Enterprise, Industrial, 6 month GenEx 6 Enterprise with Exiqon qPCR wizard, qPCR analysis software, 6 month license for industrial use
207028 Exiqon GenEx 6 Enterprise, Academic, 6 month GenEx 6 Enterprise with Exiqon qPCR wizard, qPCR analysis software, 6 month license for academic use
207029 Exiqon GenEx 6 Pro, Industrial, 12 month GenEx 6 Pro with Exiqon qPCR wizard, qPCR analysis software, 12 month license for industrial use
207030 Exiqon GenEx 6 Pro, Academic, 12 month GenEx 6 Pro with Exiqon qPCR wizard, qPCR analysis software, 12 month license for academic use
207031 Exiqon GenEx 6 Enterprise, Industrial, 12 month GenEx 6 Enterprise with Exiqon qPCR wizard, qPCR analysis software, 12 month license for industrial use
207032 Exiqon GenEx 6 Enterprise, Academic, 12 month GenEx 6 Enterprise with Exiqon qPCR wizard, qPCR analysis software, 12 month license for academic use
207033 Exiqon GenEx USB key, Pro Flexible-user license upgrade to Exiqon GenEx 6 Pro, portable USB license key. Add-on product to valid Exiqon GenEx 6 license acquired previously or concurrently
207034 Exiqon GenEx 6 Pro, Industrial, Site license GenEx 6 Pro site license for industrial use
207035 Exiqon GenEx 6 Pro, Academic, Site license GenEx 6 Pro site license for academic use
207036 Exiqon GenEx 6 Enterprise, Industrial, Site license GenEx 6 Enterprise site license for industrial use
207037 Exiqon GenEx 6 Enterprise, Academic, Site license GenEx 6 Enterprise site license for academic use


NOTICE TO PURCHASER: DISCLAIMER OF LICENSE No license is conveyed with the purchase of this product under any of US Patents Nos. 5,210,015, 5,487,972, 5,804,375, 5,994,056, 6,171,785, 6,214,979, 5,538,848, 5,723,591, 5,876,930, 6,030,787, and 6,258,569, and corresponding patents outside the United States, or any other patents or patent applications, relating to the 5’ Nuclease and dsDNA-Binding Dye Processes. For further information contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
  Privacy   Sitemap   Legal