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miRCURY™ microRNA QC PCR Panel

Quick and easy quality control of RNA samples to ensure that only optimal samples are used for your qPCR profiling studies. Use the 96 or 384 well PCR panels to check the quality of your isolated RNA, look for outlier samples, identify the presence of nucleases and PCR inhibitors and look for signs of hemolysis. Avoid spending time and resources on suboptimal samples.

Biofluid sample QC and identification of hemolyzed samples

Read more about qPCR-based sample QC and Exiqon’s unique hemolysis indicator.

Download Biofluids Guidelines
  • All-in-one solution – Use with the RNA Spike-In Kit, to evaluate sample preparation, cDNA synthesis and qPCR synthesis
  • Designed by experts – uses carefully selected biological markers and spike-ins based on millions of real data-points
  • Unique hemolysis indicator – evaluate degree of hemolysis in serum /plasma samples using microRNA biomarkers
  • Save time and money – Discover outlier samples before running your microRNA profiling project
  • Comprehensive coverage – Works with samples from human, mouse, rat, monkey and dog
  • Easy and convenient – Available in ready-to-use 96-well or 384-well plates for a wide variety of qPCR instruments

The importance of a good start

MicroRNA qPCR is the most commonly used technique for microRNA expression profiling. There are many advantages of using PCR: it is fast, sensitive and accurate. However, successful qPCR experiments depend upon high quality input RNA. Degraded RNA decreases the efficiency of the technique and inhibitors can adversely affect the cDNA synthesis and amplification steps. For some samples, such as biofluids, determining the quality of the extracted RNA can be difficult.

Exiqon’s miRCURY™ microRNA QC PCR Panel offers a unique and comprehensive way of checking sample quality and PCR efficiency. The panel contains LNA™ PCR primer sets that target microRNAs and spike-ins that can be specifically used for RNA quality control. These primer sets have been selected based on Exiqon's extensive experience in microRNA qPCR and analysis of millions of data points. 

Used in conjunction with Exiqon's spike-in kit, the panel can be used to find differences in RNA yield and to identify outlier samples (Figure 1). In addition, RNA spike-ins can be used to check the cDNA synthesis and PCR amplification efficiencies.

The panel contains assays for microRNAs that are expressed in a wide range of sample types and can therefore be used to evaluate the samples in a biological context. Two of the microRNAs in the panel can be used to evaluate the level of hemolysis such as serum and plasma.


Product coverage

The miRCURY LNA™ Universal RT microRNA QC PCR Panel consists of 12 pre-defined assays for use in human, mouse, rat, monkey and dog samples (Figure 2). It can be used for RNA from a wide variety of samples, including tissue, cell culture and bio-fluidic samples.

Coverage of the RNA QC PCR Panel

Panel layout.The miRCURY LNA™ Universal RT microRNA QC PCR Panel contains 6 LNA™-enhanced primer sets for quality control of RNA samples (light gray) and PCR efficiency (dark gray). In addition, 5 LNA™ PCR primer sets that target microRNAs are included. These assays target: microRNAs expressed in most tissues (blue), microRNAs expressed in kidney (purple) and brain (green). Primer sets targeting blood microRNAs are also part of the panel (red). These assays can be used as controls of hemolysis.

The Panel is available in two formats:
  • 96 well, Ready-to-Use - Two 96-well PCR plates containing 16 microRNA QC panels. Compatible with most real-time PCR instruments (Figure 3).
  • 384 well, Ready-to-Use - One 384-well PCR plate containing 32 microRNA QC panels. Compatible with most real-time PCR instruments (Figure 3).
The plates are produced in Ready-to-use format which means that the primer sets are dispensed and lyophilized in the well in an amount sufficient for one 10 µL reaction per well.


Figure 1 miRCURY™ microRNA QC PCR Panel
Quickly identify outlier RNA samples. (Click to learn more)


Figure 2 miRCURY™ microRNA QC PCR Panel
Coverage on the panel. (Click to learn more)


Figure 3
Instrument coverage of the panel. (Click to learn more)

A truly unique product

Exiqon’s miRCURY™ microRNA QC PCR Panel offers a unique and comprehensive way of checking sample quality and PCR efficiency. The panel is compatible with many sample types, including cell culture, tissue samples and biofluids from human, mouse, rat, monkey and dog (Figure 1).

Use the panel to test your isolated RNA to find outliers and suboptimal samples (Figure 2), including serum/plasma samples affected by hemolysis (Figure 3). This saves time and resources in the laboratory. For details , please see pages 15-23 of the miRCURY™ microRNA QC PCR Panel Instruction Manual.





















Figure 1 microRNA QC PCR Panels can be used with a wide range of sample types
microRNA QC PCR Panels can be used with a wide range of sample types. (Click to learn more)


Figure 2 Determine RNA yields using RNA spike-ins
Determine RNA yields using RNA spike-ins. (Click to learn more)


Figure 3 Determine RNA yields using RNA spike-ins
Find samples affected by hemolysis. (Click to learn more)


A unique system developed specifically for microRNA profiling

The miRCURY LNA™ Universal RT microRNA PCR System offers the best available combination of performance and ease-of-use on the market because it unites two important features (Figure 2):
  • One cDNA reaction for all microRNAs – One single Universal first strand cDNA synthesis reaction is used as template, regardless of the number of microRNAs being profiled. This saves precious sample, reduces technical variation, means less pipetting and saves time in the laboratory.
  • Two LNA™-enhanced microRNA qPCR primers – Both qPCR primers are microRNA-specific and optimized with LNA™. LNA™ primers bind with high affinity, and are shorter than standard PCR primers, so that both primers can fit on the microRNA without overlapping. The result is unrivalled sensitivity and specificity, and extremely low background.

Unmatched sensitivity

Universal RT combined with LNA™-enhanced and Tm normalized primers enables accurate and reliable quantification of individual microRNAs from as little as 1 pg total RNA (Figure 3). In comparison to other microRNA real-time PCR systems using either stem-loop or standard DNA primers, the LNA™-enhanced primers offer significantly increased sensitivity, especially for AU-rich microRNAs.

Exceptional sensitivity as well as extremely low background enables accurate quantification of very low levels of microRNA without the need for pre-amplification. This makes the miRCURY LNA™ Universal RT microRNA PCR System suitable for all sample types, and especially samples with low RNA content e.g. biofluids such as serum/plasma.

Fully validated and optimized

All miRCURY LNA™ Universal RT microRNA PCR primer sets have been optimized for maximum sensitivity and thoroughly validated either by wet lab testing or in silico. In wet lab validation, over 95% of assays detect 10 microRNA copies or less in the PCR reaction (Figure 4). The primer sets have also been validated for specific amplification of the target and for minimal background signal.

Truly specific – no false positives

The incorporation of LNA™ in the qPCR primers facilitates the design of assays that can distinguish between microRNA sequences that differ by a single nucleotide. This makes it a truly specific microRNA qPCR platform – the only microRNA platform that gives no false positive signals (Figure 5). In addition, the assays can discriminate between mature microRNA sequences and precursor microRNA (Table 1).

Fast, easy and reproducible

Save time and effort in the laboratory with the 3 hour easy-to-follow protocol that minimizes pipetting. By using the same cDNA as template in all subsequent PCR reactions, the procedure is greatly simplified compared to systems that require microRNA-specific cDNA synthesis.

With fewer pipetting steps, technical variation is also reduced. As a result, it is possible to achieve extremely high reproducibility from day-to-day (Figure 6) and even site-to-site.

Flexible qPCR system

Tailor your experimental setup to your specific need using individual assays or fully flexible custom Pick-&-Mix panels . Using the same cDNA reaction, shift seamlessly between individual primers, pre-designed miRNome and Focus panels, or custom Pick-&-Mix panels.
Figure 1 Overview of the miRCURY LNA™ Universal RT PCR workflow
Overview of the miRCURY LNA™ Universal RT PCR workflow. (Click to learn more)

Figure 2 Schematic outline of the miRCURY LNA™ Universal RT microRNA PCR System
Schematic outline of the miRCURY LNA™ Universal RT microRNA PCR System. (Click to learn more)

Figure 3 Accurate quantitation from down to 1 pg total RNA starting material
Accurate quantitation from down to 1 pg total RNA starting material. (Click to learn more)

Figure 4 Serial dilution of hsa-miR-181a
Serial dilution of hsa-miR-181a. (Click to learn more)

Figure 5 Specificity results from the miRQC Study
Specificity results from the miRQC Study. (Click to learn more)

Table 1 Discrimination between mature and precursor microRNAs
Discrimination between mature and precursor microRNAs. (Click to learn more)

Figure 6 Excellent day-to-day reproducibility
Excellent day-to-day reproducibility. (Click to learn more)

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Prod. No.
Product Name
Description Notes
203887 microRNA QC PCR Panel, 96 well (V4.RO) miRCURY LNA™ Universal RT microRNA PCR, 16 Ready-to-Use microRNA QC PCR Panels, supplied in two 96-well plates. Each panel comprises 6 microRNA primer sets and 5 reference genes and one UniSp3 for evaluation of microRNA quality. For use with Roche LightCycler 480 instruments.
203888 microRNA QC PCR Panel, 96 well (V4.AF) miRCURY LNA™ Universal RT microRNA PCR, 16 Ready-to-Use microRNA QC PCR Panels, supplied in two 96-well plates. Each panel comprises 6 LNA™ microRNA primer sets and 5 reference genes and one UniSp3 for evaluation of microRNA quality. For use with ABI Fast instrument series and ABI StepOne Plus.
203889 microRNA QC PCR Panel, 96 well (V4.MI) miRCURY LNA™ Universal RT microRNA PCR, 16 Ready-to-Use microRNA QC PCR Panels, supplied in two 96-well plates. Each panel comprises 6 LNA™ microRNA primer sets and 5 reference genes and one UniSp3 for evaluation of microRNA quality. For use with BioRad iQ series, Eppendorf, Stratagene Mx4000 and AB 7000 series instruments*.
203890 microRNA QC PCR Panel, 96 well (V4.ST) miRCURY LNA™ Universal RT microRNA PCR, 16 Ready-to-Use microRNA QC PCR Panels, supplied in two 96-well plates. Each panel comprises 6 LNA™ microRNA primer sets and 5 reference genes and one UniSp3 for evaluation of microRNA quality. For use with Stratagene Mx3000P series.
203891 microRNA QC PCR Panel, 384 well (V4.R) miRCURY LNA™ Universal RT microRNA PCR, 32 Ready-to-Use microRNA QC PCR Panels, supplied in one 384-well plate. Each panel comprises 6 LNA™ microRNA primer sets and 5 reference genes and one UniSp3 for evaluation of microRNA quality. For use with Roche LightCycler 480 instruments.
203892 microRNA QC PCR Panel, 384 well (V4.M) miRCURY LNA™ Universal RT microRNA PCR, 32 Ready-to-Use microRNA QC PCR Panels, supplied in one 384-well plate. Each panel comprises 6 LNA™ microRNA primer sets and 5 reference genes and one UniSp3 for evaluation of microRNA quality. For use with ABI 7900HT and other instruments.


NOTICE TO PURCHASER: DISCLAIMER OF LICENSE No license is conveyed with the purchase of this product under any of US Patents Nos. 5,210,015, 5,487,972, 5,804,375, 5,994,056, 6,171,785, 6,214,979, 5,538,848, 5,723,591, 5,876,930, 6,030,787, and 6,258,569, and corresponding patents outside the United States, or any other patents or patent applications, relating to the 5’ Nuclease and dsDNA-Binding Dye Processes. For further information contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
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