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microRNA and Small RNA Sequencing Service

Peter Mouritzen , Ph.D., VP R&D Send us your samples and let our Next Generation Sequencing (NGS) experts perform your NGS analysis in state-of-the-art automated laboratories with rigorous quality control and fast turn-around times and superior data analysis and interpretation. Exiqon offers a complete sequencing service including RNA isolation, library preparation, sequencing, data analysis and biological interpretation of the results in a comprehensive and easy-to-read format
Peter Mouritzen , Ph.D., VP R&D
 

Introduction to Exiqon NGS Services


Watch the webinar and learn about:
Sample requirements, library preparation and sequencing. Find out about our rigorous data analysis and biological interpretation of the results. See our recommendations for validating your NGS data and important next steps.

Watch presentation

Exiqon microRNA and Small RNA NGS Services

  • Complete sample-to-answer solution covers everything from initial consultation with microRNA experts to the final report including thorough data analysis and interpretation
  • Provide the sample, and we will take care of RNA isolation, library preparation, sequencing, data analysis and reporting
  • Close consultation with Exiqon’s microRNA and NGS experts throughout the process
  • > 8 years’ experience with microRNA profiling services
  • Sequence from as little as 250 ng total RNA
  • Rigorous quality control in all steps of the workflow
  • Fast turn-around times - delivery of the final report within 4-6 weeks of receiving your sample
  • Combine with downstream validation by market-leading LNA™-enhanced qPCR platform

A sample-to-answer solution for NGS Service

Our microRNA and small RNA NGS service includes every step in the process from initial consultation with our microRNA and small RNA experts, RNA isolation, total RNA QC, library preparation and QC, sequencing and QC, data analysis and reporting. All analyses are performed by PhD-level scientists who will ensure that you get the best service throughout the project.

Exiqon Services provide outstanding data analysis, interpretation and visualization. Our superior bioinformatics pipeline is based on solid biological expertise and microRNA and small RNA research in particular. The experimental and bioinformatics pipelines are closely integrated. This enables us to offer a market leading product that reflects the needs of the customer.

Data and results from your microRNA profiling project will be delivered in an easy-to-read report with publication grade illustrations including a summary file of the data. The encrypted raw data can be supplied.

The workflow of an Exiqon microRNA or small RNA sequencing Services project is summarized in Figure 1. Details of each step in the process are described in the following tab.


Contact us for more information

 

Figure 1 Workflow of a sequencing project
Workflow of a sequencing project. (Click to learn more)

1. Consultation

When you engage in a sequencing Service project with Exiqon, you are assured direct communication with the scientists performing your experiments throughout the duration of the project.

Each project begins with a free consultation with a microRNA expression profiling expert. Together we design an experimental setup that best satisfies your research needs and budget. Following this we will complete a detailed sample-submission form making sure that all experimental details and subsequent analysis are clearly defined and understood by both parties.

2. RNA sample submission

High quality samples are important for accurate microRNA profiling. At the initial consultation, we offer recommendations on suitable extraction and clean-up methods. Alternatively, you can take advantage of our expertise and submit your samples to our RNA isolation service. Recommended sample input is 1-2 µg total RNA, but we can get high quality results with as little as 250 ng total RNA.

3. Sample preparation

At Exiqon Services we also offer to isolate RNA from your samples for sequencing.

4. RNA sample quality control (QC)

After receiving your RNA samples, our experts will determine the integrity and quantity of each sample using Bioanalyzer and NanoDrop™ instruments. Possible contaminations are likewise assessed for each sample. You will receive a report with the results of these analyses prior to the sequencing.

5. Library preparation

Following quality control, we generate libraries and select size using an automated gel cutter allowing customized size selection.

6. Sequencing

Exiqon Services offers two types of sequencing using the Illumina platform
  • microRNA sequencing including microRNA discovery
  • smallRNA sequencing

7. Analysis & interpretation

Data Analysis including statistical analysis
  • QC of raw data
  • Mapping sequence to the genome (human, mouse or rat)
  • QC of mapped data
  • Identification of microRNAs including novel microRNAs (using microRNA prediction software miRPara)
  • Differential expression analysis (group comparison)
  • Unsupervised analysis: PCA plot and heatmap
  • Supervised analysis: The analyses that are relevant are included with your report. The comparisons could be 1) t-test (paired or unpaired) or 2) ANOVA
  • Gene Ontology (GO) enrichment analysis
  • Biological interpretation through use of Exiqon’s tools (miRSearch) to identify validated and predicted target genes, and microRNAs sharing target genes
In connection with large service projects we also offer fully extensive customized bioinformatics analysis.

8. Report and final consultation

The final report is delivered as link to our secure webserver and will contain
  • An easy-to-read data report as pdf containing a description of the project, assessments of sample and data quality and an overview of the results of the data analysis with publication-grade illustrations.
  • Data analysis summary file which is sufficient for publication, or performing your own analysis of the data if needed.
  • The complete encrypted raw data can be provided on USB3-stick at extra charge
  • Expected turnaround time: 4 - 6 weeks from samples passing QC at Exiqon Services.
Scientific follow-up can be organized in case you have questions to your final report or wish to discuss the significance of your results further.


Contact us for more information

  Figure 1 Workflow of a sequencing project
Workflow of a sequencing project. (Click to learn more)

Comprehensive report including biological interpretation

Based on our extensive microRNA and bioinformatics expertise we have developed unique in-house tools for collecting, analyzing and visualizing NGS results.


Download a report example


At the end of each project, you will receive a comprehensive and easy-to-read final report.
Download a report example



The experimental and bioinformatics pipelines are closely integrated, which enables us to offer a market leading product that reflects the needs of the customer. In addition to the standard NGS data analysis the report includes:
  • Identification of microRNAs including novel microRNAs using a combination of software for most reliable microRNA prediction
  • Unsupervised group comparisons is presented in publication-ready plots including Volcano plots, Principle component analysis and heat maps
  • Differential expression analysis is conducted and tables present the microRNAs that have significantly different reads between the groups
  • Gene Ontology (GO) enrichment analysis to determine biological significance of the data
  • Biological interpretation through use of Exiqon’s sophisticated tools (miRSearch) to identify validated and predicted target genes
  • Scientific follow-up is available if you have questions to your final report or wish to discuss the significance of your results further. Exiqon scientists can also help you design appropriate assays for validation of you NGS data.


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