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ExiLERATE LNA™ qPCR Control primer set

Validated primer sets developed for use with the ExiLERATE LNA™ qPCR system. For normalization of mRNA/ncRNA expression levels during real-time PCR analysis.

  • Accurate and reliable normalization of mRNA/ncRNA real-time PCR data
  • Optimized primer sets for the quantification of endogenous coding RNA, non-coding RNA and small nucleolar or nuclear RNA molecules
  • Validated for optimal performance with the ExiLERATE LNA™ qPCR cDNA synthesis kit and SYBR® Green master mix
  • Validated control assays targeting GapmeR positive controls are available

Coverage

22 reference gene primer sets are available to enable high quality data normalization and generation of reliable data from many different species (Table 1). These primer sets target endogenous coding RNAs, long non-coding RNA and small non-coding RNAs that are potentially constitutively expressed in a wide variety of tissues. The primer sets have been validated as reference genes for the ExiLERATE LNA™ qPCR system and work optimally with the ExiLERATE LNA™ qPCR, cDNA synthesis kit and SYBR® Green master mix.

For information regarding target organisms and sequence references, please see Table 1. 

Table 1
Product name Type Product number
HOTAIR (hsa) Non-coding long RNA 308000
ACTB (hsa, mmu, rno) Coding RNA 308001
PPIA (hsa, mmu, rno) Coding RNA 308002
MALAT1 (hsa) Non-coding long RNA 308003
PGK1 (hsa, mmu, rno) Coding RNA 308004
GAPDH (hsa, mmu, rno) Coding RNA 308005
U6 snRNA (hsa) Non-coding small RNA 308006
SNORD38B (hsa) Non-coding small RNA 308007
SNORD44 (hsa) Non-coding small RNA 308008
SNORD48 (hsa) Non-coding small RNA 308009
SNORD49A (hsa) Non-coding small RNA 308010
SNORA66 (hsa) Non-coding small RNA 308011
5S rRNA (hsa,mmu) Ribosomal RNA 308012
U6 snRNA (mmu, rno) Non-coding small RNA 308013
RNU5G (hsa, mmu, rno) Non-coding small RNA 308014
RNU1A1 (hsa, mmu, rno) Non-coding small RNA 308015
SNORD65 (mmu) Non-coding small RNA 308016
SNORD68 (mmu) Non-coding small RNA 308017
SNORD110 (mmu) Non-coding small RNA 308018
MALAT1 (mmu) Non-coding long RNA 308019
PTEN (human) Coding RNA 308020
MTOR (human) Coding RNA 308021

Unmatched sensitivity

The exceptional sensitivity of the ExiLERATE LNA™ qPCR primer sets is achieved by combining the following features:
  • Optimized design algorithm using LNA™ enhanced and Tm normalized primers
  • Universal RT with the ExiLERATE LNA™ qPCR cDNA synthesis kit
This combination enables accurate and reliable quantification of abundant mRNAs and lncRNAs from as little as 1 pg of total RNA input in the initial first-strand cDNA synthesis and efficient detection of most RNAs from 0.1 ng total RNA (Figure 1). High sensitivity is crucial for the detection of low abundance targets, such as lncRNA.

Excellent specificity

The incorporation of LNA™ in the PCR amplification primers (forward and reverse) facilitates the design of highly specific assays that can distinguish between RNA sequences that differ by only one nucleotide. In addition, the primers can be targeted freely over the target sequence despite AT content, due to the incorporation of LNA™ in the primers.

The use of ExiLERATE LNA™ qPCR SYBR® Green Master Mix, makes melt curve analysis possible, so that the specificity of an assay can be evaluated (Figure 2).

Superior performance – success guaranteed

The ExiLERATE LNA™ qPCR system offers superior performance compared to all major competitors, in terms of sensitivity (Figure 5). When testing the performance of assays designed to ten randomly selected mRNA transcripts, the ExiLERATE LNA™ qPCR assays were able to detect all targets at 1 ng total RNA input, a success rate far superior to all major competitors (Figure 6).
Enhanced by LNA™ – Success guaranteed

Note

The ExiLERATE LNA™ qPCR primers have been optimized for use with the ExiLERATE LNA™ qPCR, cDNA synthesis kit and SYBR® Green master mix kit. Use of other reagents will affect the quality of the results.

ExiLERATE guarantee

If you are unhappy with the performance of an ExiLERATE LNA™ qPCR assay, our technical support team will work with you to identify the issue and if required replace with a different assay for the same target at no extra cost. Data from specific customer experiments may be required to evaluate assay performance e.g. use of the GAPDH primer set and/or independent evidence for transcript expression. Any problem should be reported within 3 months of delivery. ExiLERATE LNA™ qPCR primers must be used with the ExiLERATE LNA™ qPCR, cDNA synthesis kit and SYBR® Green master mix kit. Exiqon reserves the right to modify the terms of this guarantee at any time.

Figure 1 ExiLERATE LNA™ qPCR system offers excellent sensitivity down to 0.1 ng total RNA
Excellent sensitivity. (Click to learn more)


Figure 2 Human GAPDH was amplified from a total RNA sample
Beautiful amplification and melting curves (human GAPDH). (Click to learn more)


Figure 3 Mouse GAPDH was amplified from a total RNA sample
Beautiful amplification and melting curves (mouse GAPDH). (Click to learn more)


Figure 4 Rat GAPDH was amplified from a total RNA sample
Beautiful amplification and melting curves (rat GAPDH). (Click to learn more)


Figure 5 ExiLERATE LNA™ qPCR sensitivity exceeds all major competitors
ExiLERATE LNA™ qPCR sensitivity exceeds all major competitors. (Click to learn more)


Figure 6 ExiLERATE LNA™ qPCR assays outperform all major competitors
ExiLERATE LNA™ qPCR assays outperform all major competitors. (Click to learn more)
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Showing 1 - 22 of 22 products
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Prod. No.
Product Name
Description Notes
308000 HOTAIR (hsa) PCR primer set, ExiLERATE ExiLERATE LNA™ qPCR control primer set, 200 rxns
308001 ACTB (hsa, mmu, rno) PCR primer set, ExiLERATE ExiLERATE LNA™ qPCR control primer set, 200 rxns
308002 PPIA (hsa, mmu, rno) PCR primer set, ExiLERATE ExiLERATE LNA™ qPCR control primer set, 200 rxns
308003 MALAT1 (hsa) PCR primer set, ExiLERATE ExiLERATE LNA™ qPCR control primer set, 200 rxns
308004 PGK1 (hsa, mmu, rno) PCR primer set, ExiLERATE ExiLERATE LNA™ qPCR control primer set, 200 rxns
308005 GAPDH (hsa, mmu, rno) PCR primer set, ExiLERATE ExiLERATE LNA™ qPCR control primer set, 200 rxns
308006 U6 snRNA (hsa) PCR primer set, ExiLERATE ExiLERATE LNA™ qPCR control primer set, 200 rxns
308007 SNORD38B (hsa) PCR primer set, ExiLERATE ExiLERATE LNA™ qPCR control primer set, 200 rxns
308008 SNORD44 (hsa) PCR primer set, ExiLERATE ExiLERATE LNA™ qPCR control primer set, 200 rxns
308009 SNORD48 (hsa) PCR primer set, ExiLERATE ExiLERATE LNA™ qPCR control primer set, 200 rxns
308010 SNORD49A (hsa) PCR primer set, ExiLERATE ExiLERATE LNA™ qPCR control primer set, 200 rxns
308011 SNORA66 (hsa) PCR primer set, ExiLERATE ExiLERATE LNA™ qPCR control primer set, 200 rxns
308012 5S rRNA (hsa, mmu) PCR primer set, ExiLERATE ExiLERATE LNA™ qPCR control primer set, 200 rxns
308013 U6 snRNA (mmu, rno) PCR primer set, ExiLERATE ExiLERATE LNA™ qPCR control primer set, 200 rxns
308014 RNU5G (hsa, mmu, rno) PCR primer set, ExiLERATE ExiLERATE LNA™ qPCR control primer set, 200 rxns
308015 RNU1A1 (hsa, mmu, rno) PCR primer set, ExiLERATE ExiLERATE LNA™ qPCR control primer set, 200 rxns
308016 SNORD65 (mmu) PCR primer set, ExiLERATE ExiLERATE LNA™ qPCR control primer set, 200 rxns
308017 SNORD68 (mmu) PCR primer set, ExiLERATE ExiLERATE LNA™ qPCR control primer set, 200 rxns
308018 SNORD110 (mmu) PCR primer set, ExiLERATE ExiLERATE LNA™ qPCR control primer set, 200 rxns
308019 MALAT1 (mmu) PCR primer set, ExiLERATE ExiLERATE LNA™ qPCR control primer set, 200 rxns
308020 PTEN (hsa) PCR primer set, ExiLERATE ExiLERATE LNA™ qPCR control primer set, 200 rxns
308021 MTOR (hsa) PCR primer set, ExiLERATE ExiLERATE LNA™ qPCR control primer set, 200 rxns


NOTICE TO PURCHASER: DISCLAIMER OF LICENSE No license is conveyed with the purchase of this product under any of US Patents Nos. 5,210,015, 5,487,972, 5,804,375, 5,994,056, 6,171,785, 6,214,979, 5,538,848, 5,723,591, 5,876,930, 6,030,787, and 6,258,569, and corresponding patents outside the United States, or any other patents or patent applications, relating to the 5’ Nuclease and dsDNA-Binding Dye Processes. For further information contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
 
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