microRNA Ready-to-Use PCR panels

Features

The microRNA Ready-to-Use PCR panels enable exceptionally sensitive high-throughput expression profiling from minimal amounts of starting material. The combination of a Universal cDNA synthesis reaction and Ready-to-Use PCR panels enables fast and easy microRNA profiling. The panels contain dried down primers for one reaction per well and simply require addition of cDNA and SYBR® Green master mix before real-time PCR amplification.

Go from sample to results in just 3 hours. See Figure 1 for an overview of the miRCURY LNA™ Universal RT microRNA PCR workflow.

The LNA™-enhanced, microRNA specific PCR primers offers 1) exceptional sensitivity as well as extremely low background enabling accurate quantitation of very low microRNA levels and 2) highly specific assays that allow discrimination between closely related microRNA sequences. For more details please see microRNA LNA™ PCR primers.

Coverage

Two panels covering 742 human microRNAs and two panels covering 752 mouse and rat microRNAs are available. Panel I for human and mouse/rat contains high-priority microRNA primer sets. These microRNAs are generally more highly expressed, more likely to be differentially expressed in disease or more often cited in the literature. Panel II contains primer sets for the most important microRNAs not available on Panel I. To view the list of primer sets in each panel, please download the plate layout files.

Both panels contain 6 reference gene primer sets , three small RNA reference genes (U6, SNORD38B and SNORD49A for human and U6 snRNA, RNU5G, RNU1A1 for mouse and rat) and three microRNA reference genes (miR-103, miR-191 and hsa-miR-423-5p or mmu-miR-423-3p). In addition, each panel contains an inter-plate calibrator in triplicate and a control primer set for detection of a synthetic RNA spike-in provided in the Universal cDNA synthesis kit.

Note:

The microRNA Ready-to-use PCR panels have been optimized for use with the Universal cDNA synthesis kit and SYBR® Green master mix kit. Use of other Reagents will affect the quality of the results.

Accurate microRNA expression profiling with minimal sample

The microRNA Ready-to-Use PCR panels enable profiling of 742 microRNAs or both Human panel I and II, from just 40ng total RNA. This makes it possible to do high quality microRNA expression profiling in samples that contain very little total RNA, such as FFPE sections and serum/plasma (Figures 1 and 2).

The panels contain fully validated and optimized LNA™-enhanced microRNA specific PCR primers. Please go to the microRNA LNA™ PCR primer section for details and experimental data covering primer set performance.
 

Fast, easy and reproducible

Save time and effort in the laboratory with the 3 hour easy-to-follow protocol. The synthesized cDNA is simply diluted, combined with the SYBR® Green master mix and aliquoted into all the wells of the 384-well PCR plate which is then ready to run in the real-time PCR instrument. The number of pipetting steps is reduced to a minimum and technical variation is minimized. As a result, it is possible to achieve extremely high reproducibility from day-to-day (Figure 3) and even site-to-site (Figure 4). Very high levels of reproducibility are even possible when profiling using difficult samples such as RNA purified from serum (Figure 5).
 

Note:

The microRNA Ready-to-use PCR panels have been optimized for use with the Universal cDNA synthesis kit and SYBR® Green master mix kit. Use of other reagents will affect the quality of the results.