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microRNA Ready-to-Use PCR panels

Marie-Louise Lunn, Product Manager Pre-aliquoted microRNA PCR primer sets in 384-well PCR plates for exceptionally sensitive and specific expression profiling. Profile up to 752 human microRNAs using only 40ng total RNA – no pre-amplification necessary. Panels are available for for human, mouse and rat microRNAs. NEW version 3 panels offer even higher sensitivity and specificity.
Marie-Louise Lunn, Ph.D., Product Manager
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IMPORTANT NOTE

Version 3 panels require the use of the new ExiLENT SYBR® Green Master Mix kit (Product no. 203402 & 203420). They are not compatible with SYBR® Green Master Mix, Universal RT (Product no. 203400 & 203450). Version 2 panels will be discontinued on August 15, 2013.
  • Available for human, mouse and rat microRNAs
  • Unmatched sensitivity – profile 752 human or 752 rodent microRNAs using only 40ng total RNA – no pre-amplification necessary
  • NEW version 3 panels offer even higher sensitivity and specificity
  • Ideal for samples with low RNA yield such as serum/plasma, FFPE sections and LCM samples
  • Fast and easy to use – just add a mix of cDNA and ExiLENT SYBR® Green master mix to the plates and run the real-time PCR
  • Highly reproducible – simple protocol minimizes technical variation
  • Reliable performance – fully validated and optimized LNA™-enhanced primers offer discrimination of closely related microRNAs and between mature and precursor microRNAs

Features

The microRNA Ready-to-Use PCR panels enable exceptionally sensitive high-throughput expression profiling from minimal amounts of starting material. The combination of a Universal cDNA synthesis reaction and Ready-to-Use PCR panels enables fast and easy microRNA profiling. The panels contain dried down primers for one reaction per well and simply require addition of cDNA and ExiLENTSYBR® Green master mix before real-time PCR amplification.

Go from sample to results in just 3 hours. See Figure 1 for an overview of the miRCURY LNA™ Universal RT microRNA PCR workflow.

The LNA™-enhanced, microRNA specific PCR primers offers 1) exceptional sensitivity as well as extremely low background enabling accurate quantitation of very low microRNA levels and 2) highly specific assays that allow discrimination between closely related microRNA sequences. For more details please see microRNA LNA™ PCR primers.

Coverage

Two panels covering 752 human microRNAs and two panels covering 752 mouse and rat microRNAs are available. Panel I for human and mouse/rat contains high-priority microRNA primer sets. These microRNAs are generally more highly expressed, more likely to be differentially expressed in disease or more often cited in the literature. Panel II contains primer sets for the most important microRNAs not available on Panel I. To view the list of primer sets in each panel, please download the plate layout files.

The miRNome panels include 6 potential reference gene primer sets , three small RNA reference genes (U6, SNORD38B and SNORD49A for human and U6 snRNA, RNU5G, RNU1A for mouse and rat) and three microRNA reference genes (miR-103-3p, miR-191-5p and hsa-miR-423-5p or mmu-miR-423-3p) all found on Panel I. In addition, Panel I also contains qPCR assays for the 5 synthetic RNAs in the RNA Spike-in kit (cel-miR-39-3p, UniSp2, UniSp4, UniSp5 and UniSp6) that may be used for monitoring the cDNA synthesis or RNA purification (UniSp6 is also provided in the cDNA Synthesis kit II). Finally, each panel contains an inter-plate calibrator in triplicate and an empty negative control.


Note:

The microRNA Ready-to-use PCR panels have been optimized for use with the Universal cDNA synthesis kit II and ExiLENT SYBR® Green master mix kit. Use of other reagents will affect the quality of the results.

Figure 1 miRCURY LNA™ Universal RT PCR workflow
Overview of the miRCURY LNA™ Universal RT PCR workflow. (Click to learn more)

Accurate microRNA expression profiling with minimal sample

The microRNA Ready-to-Use PCR panels enable profiling of 752 microRNAs or both Human panel I and II, from just 40ng total RNA. This makes it possible to do high quality microRNA expression profiling in samples that contain very little total RNA, such as FFPE sections and serum/plasma (Figures 1 and 2).

The panels contain fully validated and optimized LNA™-enhanced microRNA specific PCR primers. Please go to the microRNA LNA™ PCR primer section for details and experimental data covering primer set performance.

Fast, easy and reproducible

Save time and effort in the laboratory with the 3 hour easy-to-follow protocol. The synthesized cDNA is simply diluted, combined with the ExiLENT SYBR® Green master mix and aliquoted into all the wells of the 384-well PCR plate which is then ready to run in the real-time PCR instrument. The number of pipetting steps is reduced to a minimum and technical variation is minimized. As a result, it is possible to achieve extremely high reproducibility from day-to-day (Figure 3) and even site-to-site (Figure 4). Very high levels of reproducibility are even possible when profiling using difficult samples such as RNA purified from serum (Figure 5).

Note:

The microRNA Ready-to-use PCR panels have been optimized for use with the Universal cDNA synthesis kit II and ExiLENT SYBR® Green master mix kit. Use of other reagents will affect the quality of the results.
Figure 1 Profile 730 miRNA from 40 ng total RNA
Expression profiling of 730 microRNAs using 40 ng total RNA. (Click to learn more)

Figure 2 miRNA profiling in serum
Profiling of 368 microRNAs from 35μl serum. (Click to learn more)

Figure 3 Excellent day-to-day reproducibility
Excellent day-to-day reproducibility. (Click to learn more)

Figure 4 Excellent site-to-site correlation
Excellent reproducibility between different sites. (Click to learn more)

Figure 5 miRNA qPCR reproducibility
Excellent reproducibility between RT reactions on total RNA from serum. (Click to learn more)
Products ready to order Click to view pricing
Showing 1 - 8 of 8 products
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Prod. No.
Product Name
Description Notes
203611 microRNA Ready-to-Use PCR, Human panel I+II, V3.M miRCURY LNA™ Universal RT microRNA PCR, 4x Human panel I+II in 384well PCR plates (type M), one reaction each of 752 human microRNAs plus control assays . For use with ABI 7900HT and other instruments. Use with ExiLENT master mix only[!]
203612 microRNA Ready-to-Use PCR, Human panel I+II, V3.R miRCURY LNA™ Universal RT microRNA PCR, 4x Human panel I+II in 384well PCR plates (type R), one reaction each of 752 human microRNAs plus control assays. For use with Roche LightCycler 480 instruments. Use with ExiLENT master mix only[!]
203613 microRNA Ready-to-Use PCR, Human panel I, V3.M miRCURY LNA™ Universal RT microRNA PCR, 4x Human panel I in 384well PCR plates (type M), one reaction each of 372 human microRNAs plus control assays. For use with ABI 7900HT and other instruments. Use with ExiLENT master mix only[!]
203614 microRNA Ready-to-Use PCR, Human panel I, V3.R miRCURY LNA™ Universal RT microRNA PCR, 4x Human panel I in 384well PCR plates (type R), one reaction each of 372 human microRNAs plus control assays. For use with Roche LightCycler 480 instruments. Use with ExiLENT master mix only[!]
203709 microRNA Ready-to-Use PCR, Mouse&Rat panel I+II, V3.M miRCURY LNA™ Universal RT microRNA PCR, 4x Mouse&Rat panel I+II in 384well PCR plates (type M), one reaction each of 752 Mouse&Rat microRNAs plus control assays. For use with ABI 7900HT and other instruments. Use with ExiLENT master mix only[!]
203710 microRNA Ready-to-Use PCR, Mouse&Rat panel I+II, V3.R miRCURY LNA™ Universal RT microRNA PCR, 4x Mouse&Rat panel I+II in 384well PCR plates (type R), one reaction each of 752 Mouse&Rat microRNAs plus control assays. For use with Roche LightCycler 480 instruments. Use with ExiLENT master mix only[!]
203711 microRNA Ready-to-Use PCR, Mouse&Rat panel I, V3.M miRCURY LNA™ Universal RT microRNA PCR, 4x Mouse&Rat panel I in 384well PCR plates (type M), one reaction each of 372 Mouse&Rat microRNAs plus control assays. For use with ABI 7900HT and other instruments. Use with ExiLENT master mix only[!]
203712 microRNA Ready-to-Use PCR, Mouse&Rat panel I, V3.R miRCURY LNA™ Universal RT microRNA PCR, 4x Mouse&Rat panel I in 384well PCR plates (type R), one reaction each of 372 Mouse&Rat microRNAs plus control assays. For use with Roche LightCycler 480 instruments. Use with ExiLENT master mix only[!]


NOTICE TO PURCHASER: LIMITED LICENSE Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,994,056 and 6,171,785. The purchase of this product includes a limited, nontransferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
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