NEW: Universal cDNA Synthesis and SYBR® Green Master Mix kits

Universal cDNA Synthesis kit

The kit contains reagents for polyandenylation and reverse transcription of all microRNAs into cDNA in a single reaction step. By using the same RT reaction as template in all subsequent PCR reactions, the procedure is greatly simplified compared to systems that require microRNA-specific first-strand synthesis (Figure 1).

The kit contains enough reagents for 32 20µl reverse transcription reactions. Please note that for screening both microRNA Ready-to-Use human panel I and II, two RT reactions are required.

The following materials are supplied: 5x reaction buffer, 10x enzyme mix, nuclease free water and a synthetic RNA spike-in template. The RNA spike-in can be used with the Control primer set provided in the SYBR® Green master mix, Universal RT or in the microRNA Ready-to-use PCR panels as a positive control and/or as an inter-plate calibrator. Please refer to the miRCURY LNA™ Universal RT microRNA PCR Instruction manual for details.

SYBR® Green Master mix, Universal RT

The SYBR® Green Master mix is available in two sizes. The 2.5 ml kit contains enough reagents for 250 20µl PCR reactions or 500 10µl PCR reactions (please note that the recommended reaction volume in PCR tubes or 96well plates is 20µl and the recommended reaction volume in 384-well plates is 10µl). The 25ml kit contains enough reagents for 2500 20µl reactions or 5000 10µl reactions.

Both kits contain the appropriate volume of 2x SYBR® Green master mix, nuclease-free water and a Control primer set. The Control primer set is designed for amplification of the synthetic RNA spike-in provided in the Universal cDNA synthesis kit. Please refer to the miRCURY LNA™ Universal RT microRNA PCR Instruction manual for details.

Click here for information regarding the microRNA Ready-to-use PCR panels or LNA™ PCR primer sets.

The reagents below are compatible with the NEW Universal RT based PCR system ONLY. Click here to order reagents kits from the old PCR system based on gene-specific RT.

Universal cDNA Synthesis kit

The kit contains reagents for fast and reliable reverse transcription of all microRNAs into cDNA in a single reaction step. The number of pipetting steps are kept to a minimum in order to save time and minimize technical variation. For experimental data, please see the pages covering microRNA LNA™ PCR primer sets and microRNA Ready-to-use panels.

SYBR® Green Master mix, Universal RT

This kit contains high performance reagents for quantitative real-time PCR amplification. The detection is based on SYBR® Green which allows quality control of the resulting PCR amplicon by melt curve analysis. The LNA™-enhanced primer sets require specific PCR reaction conditions for optimal performance. The primer sets have been developed and validated using the SYBR® Green master mix , Universal RT and the use of other reagents for real-time PCR will affect the quality of the results (Figure 1).

Click here for further experimental data using the microRNA Ready-to-use PCR panels or LNA™ PCR primer sets.

The reagents below are compatible with the NEW Universal RT based PCR system ONLY. Click here to order reagents kits from the old PCR system based on gene-specific RT.