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Reference Gene Primer Sets

Validated primer sets developed for use with the microRNA LNA™ PCR primer sets. For normalization of microRNA expression levels during real-time PCR analysis.


New microRNA PCR starter kit available

We are very happy to introduce our new starter kit which gives you the chance to experience the superior performance of our microRNA qPCR system in your own hands. Find out more
  • Accurate and reliable normalization of microRNA real-time PCR data
  • Optimized primer sets for the quantification of endogenous small nucleolar or nuclear RNA molecules
  • Validated for optimal performance with the Universal cDNA synthesis kit and SYBR® Green master mix, Universal RT

Coverage

Nine reference gene primer sets are available to enable high quality data normalization and generation of reliable data from many different sample types (Table 1). These primer sets target endogenous small non-coding RNAs that are constitutively expressed in a wide variety of tissues. The primer sets have been validated as reference genes for the miRCURY LNA™ Universal RT microRNA PCR system and work optimally with the Universal cDNA synthesis kit and SYBR® Green master mix , Universal RT.

For information regarding target organisms and sequence references, please see Table 1.

MicroRNA primer sets can also be used as references. For examples, see these tech notes: Click to view information regarding microRNA LNA™ PCR primer sets


Table 1 Available reference genes
Available reference genes. (Click to learn more)

Reference genes for normalization

miRCURY LNA™ Universal RT microRNA PCR Reference genes are constitutively and relatively stably expressed across different human tissues (Figure 1). The reference genes cover a large range of Cq values offering the possibility to accurately and reliably normalize across a range of microRNA expression levels.

It is very important to always confirm the consistent and unvarying expression of a gene in all tissues and under all treatments of an experiment before choosing it as the reference. It is recommended to test a number of reference genes and use software such as GeNorm and NormFinder (found as part of the GenEx software) to choose and validate reference genes.

View experimental data describing miRCURY LNA™ Universal RT microRNA PCR

Figure 1 Expression levels of reference genes in different human tissues
Expression levels of reference genes in different human tissues. (Click to learn more)
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Showing 1 - 12 of 12 products
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Prod. No.
Product Name
Description Notes
203901 SNORD38B (hsa) PCR primer set, UniRT miRCURY LNA? Universal RT microRNA PCR, reference gene primer set, 200 rxns [!]
203902 SNORD44 (hsa) PCR primer set, UniRT miRCURY LNA? Universal RT microRNA PCR, reference gene primer set, 200 rxns [!]
203903 SNORD48 (hsa) PCR primer set, UniRT miRCURY LNA? Universal RT microRNA PCR, reference gene primer set, 200 rxns [!]
203904 SNORD49A (hsa) PCR primer set, UniRT miRCURY LNA? Universal RT microRNA PCR, reference gene primer set, 200 rxns [!]
203905 SNORA66 (hsa) PCR primer set, UniRT miRCURY LNA? Universal RT microRNA PCR, reference gene primer set, 200 rxns [!]
203906 5S rRNA (hsa,mmu) PCR primer set, UniRT miRCURY LNA? Universal RT microRNA PCR, reference gene primer set, 200 rxns [!]
203907 U6 snRNA (hsa, mmu, rno) PCR primer set, UniRT miRCURY LNA? Universal RT microRNA PCR, reference gene primer set, 200 rxns [!]
203908 RNU5G (hsa, mmu, rno) PCR primer set, UniRT miRCURY LNA? Universal RT microRNA PCR, reference gene primer set, 200 rxns [!]
203909 RNU1A1 (hsa, mmu, rno) PCR primer set, UniRT miRCURY LNA? Universal RT microRNA PCR, reference gene primer set, 200 rxns [!]
203910 SNORD65 (mmu) PCR primer set, UniRT miRCURY LNA? Universal RT microRNA PCR, reference gene primer set, 200 rxns [!]
203911 SNORD68 (mmu) PCR primer set, UniRT miRCURY LNA? Universal RT microRNA PCR, reference gene primer set, 200 rxns [!]
203912 SNORD110 (mmu) PCR primer set, UniRT miRCURY LNA? Universal RT microRNA PCR, reference gene primer set, 200 rxns [!]


NOTICE TO PURCHASER: DISCLAIMER OF LICENSE No license is conveyed with the purchase of this product under any of US Patents Nos. 5,210,015, 5,487,972, 5,804,375, 5,994,056, 6,171,785, 6,214,979, 5,538,848, 5,723,591, 5,876,930, 6,030,787, and 6,258,569, and corresponding patents outside the United States, or any other patents or patent applications, relating to the 5’ Nuclease and dsDNA-Binding Dye Processes. For further information contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
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