miRCURY LNA™ microRNA Array Data Analysis

ImaGene® 9 and Nexus Expression™ 2 in a package specifically adapted to Exiqon’s microarrays
Easy workflow for fast and accurate analysis of your miRCURY LNA™ microRNA Array data – no bioinformatics skills required
Best-in-class grid placement, automatic analysis and quality check of all spots
Quick and easy identification of differentially expressed microRNAs
Direct links to microRNAs in miRBase and TargetScan

Leading-edge microarray analysis software

Exiqon offers a comprehensive microarray analysis package specifically suited for the analysis of miRCURY LNA™ microRNA Array data. The package consists of the well-known ImaGene® 9 and Nexus Expression™ 2 from BioDiscovery as well as settings files for rapid and easy analysis of Exiqon microarrays.

It lets researchers quickly identify differentially expressed microRNAs and analyze expression patterns across samples.

A complete data analysis tool – converts raw signal values into meaningful information visualized by common statistical methods through a user-friendly workflow.

ImaGene® 9

ImaGene® is a very powerful tool for microarray image analysis. Through an intuitive interface, it lets the user extract signal intensities from the scanned array and flag (poor) spots either automatically or manually (Figure 1). ImaGene® can also be used for easy visualization of microarray data in scatter or M-A plots (Figure 2).

Highlights include:

Advanced spot finding and grid placement
Automatic analysis and quality check of all spots
Interactive spot checking
Intuitive interface and navigation
Scanner, array and system independent
Integrated settings file for convenient analysis of Exiqon’s miRCURY LNA™ microRNA Arrays
A quality control template specifically designed for Exiqon's microarrays.

Nexus Expression™ 2

Nexus Expression™ is a very feature packed but easy to use program for the analysis of microarray experiments. Using a simple workflow, raw data from ImaGene® is background-subtracted and normalized, after which the data can be visualized in heat maps and differentially expressed microRNAs identified (Figures 3 & 4).

Highlights include:

Simple but powerful workflow – No bioinformatics/programming skills required
One click workflow, with direct links to microRNAs in miRBase and TargetScan
Combines replicate measurements
Identifies differentially expressed microRNAs
Clusters analysis: clusters sample groups or microRNAs

Figure 1

Fast and accurate spot identification. (Click to learn more)

Figure 2 ImaGene® 9 screenshot

Excellent data visualization. (Click to learn more)

Figure 3 Nexus Expression™ 2 screenshot

Up or down regulated microRNAs are easily identified. (Click to learn more)

Figure 4 Nexus Expression™ 2 screenshot

Flexible clustering of data. (Click to learn more)

System requirements

ImaGene® 9

Platforms Supported: Windows 9x/Nt4/Win2k/WinXP, Linux, OS X
Minimum: 700 MHz Pentium or G4, 256 MB RAM
Recommended: 2.0 GHz or faster Intel® Core™ 2 Duo processor, 1024 or more MB RAM

Nexus Expression™ 2

Platforms Supported: Windows Win2k/WinXP, OS X
Minimum: 1.0 GHz Pentium or G4, 512 MB RAM
Recommended: 2.0 GHz or faster Intel® Core™ 2 Duo processor, 1024 or more MB RAM

Get started with the miRCURY LNA™ microRNA Array Analysis Software

1. Buy a license. Use the web shop below or contact us

2. Download the software from BioDiscovery’s website:
Windows:

Mac OS X:

3. Install both programs on your computer

4. Activate the software from BioDiscovery’s website. Use the serial number you received from step 1.
Note, day 1 of the 30-day license is counted from the day you activated the software.

5. Download settings files for Exiqon arrays (right click on link and choose "Save Target as")

Quality control of your data

As an exlusive offer to Exiqon customers, we are offering a Microsoft Excel 2007 QC template to analyze all slides in an array experiment. This template can be used to calculate various QC parameters and to determine if slides need to be removed from the experiment.