miRCURY LNA™ Endogenous Control Primer Sets

Validated primer sets developed for use with the miRCURY LNA™ microRNA Primer Sets for normalization of microRNA expression levels during real-time PCR analysis
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Ina K. Dahlsveen, Ph.D., Product Manager Back

Optimized normalization of microRNA quantitation data using the miRCURY LNA™ microRNA PCR System
Accurate and sensitive quantitation of endogenous small nucleolar and nuclear RNA molecules
Designed to work optimally with the miRCURY LNA™ First-strand cDNA Kit and miRCURY LNA™ SYBR® Green Master Mix

Coverage

A panel of miRCURY LNA™ Endogenous control primer sets targeting selected small nucleolar RNAs (snoRNAs), U6 snRNA and 5S rRNA is available (Table 1). These primer sets have been developed and validated for use with the miRCURY LNA™ microRNA primer sets for normalization of microRNA expression levels in real-time PCR experiments. Please see Table 1 for information regarding product numbers, targeted RNAs and target organisms.

Note

In addition to this product, a miRCURY LNA™ endogenous control assay also requires the following reagents:

Click to view information on miRCURY LNA™ microRNA Primer Sets

Table 1

miRCURY LNA™ Endogenous control primer sets. (Click table to enlarge)

Expression profiles of our endogenous controls

The miRCURY LNA™ Endogenous controls show comparatively stable expression levels across a panel of normal and tumor tissues illustrating their applicability for normalization of microRNA real-time PCR data (Figure 1). Figure 2 shows the stable expression profiles of five miRCURY LNA™ Endogenous controls across ten different mouse tissues.
However, it is recommended to test and validate several controls in order to find the most appropriate candidate for each microRNA quantitation study.

For further details and guidelines regarding normalization, please see the Endogenous controls technical note and the miRCURY LNA™ microRNA PCR system instruction manual.

View experimental data describing the miRCURY LNA™ microRNA PCR system .

Figure 1

The expression profiles of the miRCURY LNA™ endogenous controls are similar in cancer and normal tissues. (Click image to enlarge)

Figure 2

The expression profiles of five miRCURY LNA™ endogenous controls across a panel of ten different mouse tissues. (Click image to enlarge)

"the scientists at Exiqon were very, very supportive"

Dr. Dorina Veliceasa, from the department of Urology at the Northwestern University Feinberg School of Medicine, discusses the use of the miRCURY LNA™ microRNA PCR system in her investigation of tumor angiogenesis in endothelial cells. Dr. Veliceasa discusses the challenges of qPCR monitoring of microRNA species, including the importance of endogenous controls, and offers advice to other researchers interested in this method of determining microRNA expression levels.

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