Reference gene primer sets

Coverage

Nine reference gene primer sets are available to enable high quality data normalization and generation of reliable data (Table 1). These primer sets target endogenous small non-coding RNAs that are constitutively expressed in a wide variety of tissues. The primer sets have been validated as reference genes for the miRCURY LNA™ Universal RT microRNA PCR system and work optimally with the Universal cDNA synthesis kit and SYBR® Green master mix , Universal RT.

For information regarding target organisms and sequence references, please see Table 1.

Click to view information regarding microRNA LNA™ PCR primer sets

Reference genes for normalization

miRCURY LNA™ Universal RT microRNA PCR Reference genes are constitutively and relatively stably expressed across different human tissues (Figure 1). The reference genes cover a large range of Cq values offering the possibility to accurately and reliably normalize across a range of microRNA expression levels.

It is very important to always confirm the consistent and unvarying expression of a gene in all tissues and under all treatments of an experiment before choosing it as the reference. It is recommended to test a number of reference genes and use software such as GeNorm and NormFinder (found as part of the GenEx software) to choose and validate reference genes.

View experimental data describing miRCURY LNA™ Universal RT microRNA PCR