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miRCURY LNA™ microRNA Detection Probes

LNA™-enhanced detection probes for ultra-sensitive and specific Northern blotting of microRNAs.

  • Superior sensitivity and specificity
  • Results in just a few hours
  • microRNA detection with as little as 2.5 µg total RNA
  • Probes available for all known microRNAs

microRNA detection probes with superior sensitivity and specificity

miRCURY LNA™ microRNA Detection Probes for Northern Blotting offer very high binding affinity, single-nucleotide discrimination and remarkable signal-to-noise ratios, resulting in highly specific and sensitive microRNA detection. The probes are designed with optimal LNA™ positioning to achieve high sequence specificity, low secondary structure and minimal self-annealing.

Coverage

Pre-designed miRCURY LNA™ microRNA Detection Probes for Northern blotting are available for most known microRNAs in invertebrates, vertebrates and plants annotated in miRBase. The probes are available with a selection of 3’-end, 5’-end and double (3’ and 5’) labels: DIG, biotin, and fluorescein (FAM version). “Ready-to-label” probes designed for custom labeling are also available.

Content

Pre-designed miRCURY LNA™ microRNA Detection Probes are provided in 1 nmol quantity, delivered dried down. For larger amounts, 10 nmol probes are available as custom miRCURY LNA™ microRNA Detection Probes. Please contact us for details.

Higher sensitivity with double-labeled probes

For researchers who wish to detect microRNAs on Northern blots by non-radioactive methods, we recommend the use of double (3’ and 5’) DIG-labeled or fluorescein probes.

Note

Custom miRCURY LNA™ microRNA Detection Probes are available for your own microRNAs and other small RNAs, including pre-microRNAs. Positive and negative control probes are also available.

To find a particular miRCURY LNA™ microRNA Detection Probe, please use the search function below.

Improved sensitivity

The high specificity and sensitivity of the miRCURY LNA™ microRNA Detection Probes mean that Northern blot sample sizes can be reduced to 1/10 of that used with traditional DNA probes. Moreover, it means that exposure times can be reduced to just a few hours (Figure 1).

Using miRCURY LNA™ microRNA Detection Probes, double or even single mismatches are readily discriminated (Figure 2).
Figure 1 Northern blot analysis of two duplicate dilution series of A. thaliana
LNA™ probes are better than DNA probes at detecting microRNAs. (Click to learn more)

Figure 2 Northern blot showing high specificity using miRCURY LNA™ Detection Probes.
LNA™ readily discriminates between single nucleotide differences in the target sequence. (Click to learn more)
Ronald H. A. Plasterk

MicroRNA Involvment in Embryo Development


"We got some beautiful data and spectacular images. We generated a complete catalogue of images showing the temporal and spatial expression patterns of 115 conserved microRNAs in zebrafish embryos."

Prof Ronald H. A. Plasterk, Hubrecht Laboratory, The Netherlands.

Read full story...

Dylan Sweetman

MicroRNAs in Muscle Cell Differentiation


"Following the success of LNA™ probes as demonstrated in Plasterk's lab, we decided to take the same approach."

Dr. Dylan Sweetman is working in Dr. Andrea Münsterberg's group at the University of East Anglia, School of Biological Sciences. The group investigates cellular and molecular mechanisms that underlie embryonic development.

Read full story...

Parker Antin

MicroRNAs and Early Embryonic Development


"LNA™ technology is superior to any other for the detection of small RNA species."

Dr. Parker Antin's lab at the University of Arizona runs a large scale in situ hybridization database project to determine the expression patterns for all differentially expressed genes in the chicken embryo. Find out what the challenges were with this huge project and how they were overcome.

Read full story...

Products ready to order
Product No.:
miRNA name:
Sequence (miRNA):

Note:
Searches using “miRNA name” may return products with a miRNA name from a different species (typically hsa-miR-xxxx) with an identical target sequence. To see all microRNAs targeted by the detection probe, click on the product and go to the “Targeted microRNAs” tab.

Search tips:
You can search our products database in three ways. The easiest procedure is simply using the name of the microRNA under study:
Enter the mature microRNA name (e.g. hsa-miR-142-3p). It is also possible to search using parts of microRNA names, e.g., "hsa-let" to see all probes related to the hsa-let7 microRNA family.

If no results are returned please check that the entered information is accurate or entered in the correct field. If the problem persists please contact our technical support.
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