miRCURY LNA™ microRNA Detection Probes for Northern blotting

LNA™-enhanced detection probes for ultra-sensitive and specific Northern blotting of microRNAs

Ina K. Dahlsveen, Ph.D., Product ManagerBack

Superior sensitivity and specificity
Results in just a few hours
microRNA detection with as little as 2.5 µg total RNA
Probes available for all known microRNAs

Features

Pre-designed miRCURY LNA™ microRNA Detection Probes for Northern blotting are available for all known microRNAs in invertebrates, vertebrates and plants as registered and annotated in the microRNA registry (miRBase) at the Wellcome Trust Sanger Institute.

Each predesigned probe is designed for optimal LNA™ positioning resulting in high sequence specificity, low secondary structure and minimal self-annealing. The melting temperatures ( Tm) of the probe:target duplexes are designed to be within the 70-85ºC range.

Standard probes are available with a selection of 3’ and 5’ labels (DIG*, biotin, fluorescein, amino). “Ready-to-label” probes designed for custom labeling are also available.

For researchers who wish to detect microRNAs on Northern blots by non-radioactive methods, we recommend the use of DIG*-labeled probes.

Coverage

Custom miRCURY LNA™ microRNA Detection Probes are made to order. Positive and negative control probes are also available.

*Licensed from Roche Diagnostics GmbH

To find a particular miRCURY LNA™ microRNA Detection Probe, please use the search function below.

Improved sensitivity

The high specificity and sensitivity of the miRCURY LNA™ microRNA Detection Probes mean that Northern blot sample sizes can be reduced to 1/10 of that used with traditional DNA probes. Moreover, it means that exposure times can be reduced to just a few hours (Figure 1).

Using miRCURY LNA™ microRNA Detection Probes, double or even single mismatches are readily discriminated (Figure 2).

Figure 1

Northern blot analysis of two duplicate dilution series of A. thaliana

LNA™ probes are better than DNA probes at detecting microRNAs. (Click to learn more)

Figure 2 Northern blot showing high specificity using miRCURY LNA™ Detection Probes.

Northern blot showing high specificity using miRCURY LNA™ Detection Probes.

LNA™ readily discriminates between single nucleotide differences in the target sequence. (Click to learn more)

“We got some beautiful data and spectacular images”

"We generated a complete catalogue of images showing the temporal and spatial expression patterns of 115 conserved microRNAs in zebrafish embryos."

Prof Ronald H. A. Plasterk, Hubrecht Laboratory, The Netherlands

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“Following the success of LNA™ probes as demonstrated in Plasterk’s lab, we decided to take the same approach”

Dr. Dylan Sweetman is working in Dr. Andrea Münsterberg’s group at the University of East Anglia, School of Biological Sciences. The group investigates cellular and molecular mechanisms that underlie embryonic development.

Read full story...

“LNA™ technology is superior to any other for the detection of small RNA species”

Dr. Parker Antin’s lab at the University of Arizona runs a large scale in situ hybridization database project to determine the expression patterns for all differentially expressed genes in the chicken embryo. Find out what the challenges were with this huge project and how they were overcome.

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Products ready to order

Product No.:
miRNA name:
Sequence (miRNA):

Note:
Searches using “miRNA name” may return products with a miRNA name from a different species (typically hsa-miR-xxxx) with an identical target sequence. To see all microRNAs targeted by the detection probe, click on the product and go to the “Targeted microRNAs” tab.

Search tips:
You can search our products database in three ways. The easiest procedure is simply using the name of the microRNA under study:
Enter the mature microRNA name (e.g. hsa-miR-142-3p). It is also possible to search using parts of microRNA names, e.g., "hsa-let" to see all probes related to the hsa-let7 microRNA family.

If no results are returned please check that the entered information is accurate or entered in the correct field. If the problem persists please contact our technical support.

Scientific publications

	Gao et al. Nature 2009
	Kim et al. Nucleic Acids Res. 2010
	Mencía et al. Nat. Genet. 2009
	List of publications
	MicroRNA research 2008 booklet