LNA™-enhanced custom probes for optimal detection of any microRNA
Custom miRCURY LNA™ microRNA Detection probes for Northern Blotting are available for specific detection of novel microRNAs, microRNAs not covered by any pre-designed miRCURY LNA™ microRNA Detection probes, or other short ncRNA sequences. These custom microRNA detection probes are designed by our technical support scientists applying the same design rules used with our pre-designed microRNA detection probes.
The custom miRCURY LNA™ microRNA Detection probes are designed with optimal LNA™ positioning to achieve high sequence specificity, low secondary structure and minimal self-annealing. For Northern Blotting, this results in probes with very high binding affinity, single-nucleotide discrimination and remarkable signal-to-noise ratios, resulting in highly specific and sensitive microRNA detection.
The custom miRCURY LNA™ microRNA Detection probes are available in two different amounts (1 nmol and 10 nmol), and with a variety of 3’-end, 5’-end, or double (3’ and 5’) labeling options: DIG, biotin, fluorescein, amino, TYE563 and TYE665. “Ready-to-label” probes designed for custom labeling are also available. For ordering, price information and requests for alternative labels, please contact us
Higher sensitivity with double-labeled probes
For researchers who wish to detect microRNAs on Northern blots by non-radioactive methods, we recommend the use of double (3’ and 5’) DIG-labeled or fluorescein probes.
Before submitting your order, please check if a pre-designed miRCURY LNA™ microRNA Detection probe targeting your sequence is available
. Pre-designed miRCURY LNA™ microRNA Detection Probes are available for most microRNA sequences annotated in miRBase