miRCURY LNA™ Detection Control Probes Positive and negative control probes for quality assessment of your miRCURY LNA™ Northern blot analysis or in situ hybridization experiment
Ina K. Dahlsveen, Ph.D., Product ManagerBack | |
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- Similar in probe length and LNA™ design to our miRCURY LNA™ microRNA Detection Probes
- Both positive and negative control probes are available
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Coverage Three pre-designed miRCURY LNA™ Detection Control Probes are available: - Scramble-miR control probe (negative control). No homology with sequences in the miRBase database. No hits of >70% homology to any sequence in any organism in the NCBI database.
- U6 small nuclear RNA control probe (positive control). Designed to work for human, mouse and rat samples. Can be used for several other species, but BLAST searching is recommended before use.
- Sense miR-159 control probe (negative control). No homology with sequences in the miRBase database. No hits of >70% homology to any non-plant sequences in the NCBI database. Not recommended for use in plants.
All three control probes are available with the same selection of 3’ and 5’ labels as the miRCURY LNA™ microRNA Detection Probes (DIG*, biotin, fluorescein, amino). “Ready-to-label” probes designed for custom labeling are also available .
*Licensed from Roche Diagnostics GmbH
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Control probes for validation of results The miRCURY LNA™ Detection Control Probes are designed for use with our miRCURY LNA™ Detection Probes for both in situ hybridization and Northern blots. The control probes are similar in length and LNA™ design to the microRNA detection probes and therefore fall within the same Tm range. The U6 positive control and the scramble negative control can be used during optimization and troubleshooting of in situ hybridization experiments. In addition, these controls can be used to validate experimental results, as seen in Figure 1. |
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Figure 1
MicroRNAs are readily detected using miRCURY LNA™ Detection Probes. (Click to learn more) |
 "Exiqon is one of the leading suppliers for miRNA-related products"
Dr. Liming Luan Dr. Liming Luan at Vanderbilt University Medical Center in Nashville studies the role of microRNAs in normal and pathological conditions of the skin.
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 "I was intrigued by this interesting technology, which is very different from other available technologies."
Dr. Mircea Ivan at Indiana University School of Medicine studies the role of microRNAs in hypoxia. Here he describes why his lab chose Exiqon's detection probes.
Read full story... |
 “We got some beautiful data and spectacular images”
"We generated a complete catalogue of images showing the temporal and spatial expression patterns of 115 conserved microRNAs in zebrafish embryos."
Prof Ronald H. A. Plasterk, Hubrecht Laboratory, The Netherlands
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 “Following the success of LNA™ probes as demonstrated in Plasterk’s lab, we decided to take the same approach” Dr. Dylan Sweetman is working in Dr. Andrea Münsterberg’s group at the University of East Anglia, School of Biological Sciences. The group investigates cellular and molecular mechanisms that underlie embryonic development. |
 “LNA™ technology is superior to any other for the detection of small RNA species” Dr. Parker Antin’s lab at the University of Arizona runs a large scale in situ hybridization database project to determine the expression patterns for all differentially expressed genes in the chicken embryo. Find out what the challenges were with this huge project and how they were overcome. |
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