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miRCURY LNA™ microRNA Detection Control Probes

Positive and negative control probes for quality assessment of your miRCURY LNA™ Northern blot analysis or in situ hybridization experiment.

  • Similar in probe length and LNA™ design to our miRCURY LNA™ microRNA Detection Probes
  • Both positive and negative control probes are available

Coverage

Three pre-designed miRCURY LNA™ Detection Control Probes are available:
  1. Scramble-miR control probe (negative control). No homology with sequences in the miRBase database. No hits of >70% homology to any sequence in any organism in the NCBI database.
  2. U6 small nuclear RNA control probe (positive control). Designed to work for human, mouse and rat samples. Can be used for several other species, but BLAST searching is recommended before use.
  3. Sense miR-159 control probe (negative control). No homology with sequences in the miRBase database. No hits of >70% homology to any non-plant sequences in the NCBI database. Not recommended for use in plants.
All three control probes are available with the same selection of 3’ and 5’ labels as the miRCURY LNA™ microRNA Detection Probes (DIG, biotin, fluorescein, amino). “Ready-to-label” probes designed for custom labeling are also available .
 

Control probes for validation of results

The miRCURY LNA™ Detection Control Probes are designed for use with our miRCURY LNA™ Detection Probes for both in situ hybridization and Northern blots. The control probes are similar in length and LNA™ design to the microRNA detection probes and therefore fall within the same Tm range. The U6 snRNA positive control and the scramble negative control can be used during optimization and troubleshooting of in situ hybridization experiments. In addition, these controls can be used to validate experimental results, as seen in Figure 1.
Figure 1
Detection of has miR 127
MicroRNAs are readily detected using miRCURY LNA™ Detection Probes. (Click to learn more)
Liming Luan

MicroRNAs in Skin


"Exiqon is one of the leading suppliers for miRNA-related products."

Dr. Liming Luan at Vanderbilt University Medical Center in Nashville studies the role of microRNAs in normal and pathological conditions of the skin.

Read full story...

Mircea Ivan

MicroRNAs in Hypoxia


"I was intrigued by this interesting technology, which is very different from other available technologies."

Dr. Mircea Ivan at Indiana University School of Medicine studies the role of microRNAs in hypoxia. Here he describes why his lab chose Exiqon's detection probes.

Read full story...

Ronald H. A. Plasterk

MicroRNA Involvment in Embryo Development


"We got some beautiful data and spectacular images. We generated a complete catalogue of images showing the temporal and spatial expression patterns of 115 conserved microRNAs in zebrafish embryos."

Prof Ronald H. A. Plasterk, Hubrecht Laboratory, The Netherlands.

Read full story...

Dylan Sweetman

MicroRNAs in Muscle Cell Differentiation


"Following the success of LNA™ probes as demonstrated in Plasterk's lab, we decided to take the same approach."

Dr. Dylan Sweetman is working in Dr. Andrea Münsterberg’s group at the University of East Anglia, School of Biological Sciences. The group investigates cellular and molecular mechanisms that underlie embryonic development.

Read full story...

Parker Antin

MicroRNAs and Early Embryonic Development


"LNA™ technology is superior to any other for the detection of small RNA species."

Dr. Parker Antin's lab at the University of Arizona runs a large scale in situ hybridization database project to determine the expression patterns for all differentially expressed genes in the chicken embryo. Find out what the challenges were with this huge project and how they were overcome.

Read full story...

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Prod. No.
Product Name
Description Notes
699002-300 U6, hsa/mmu/rno miRCURY LNA™ Detection probe, positive control, 1 nmol, ready to label
699002-310 U6, hsa/mmu/rno miRCURY LNA™ Detection probe, positive control, 1 nmol, 5`-fluorescein labeled
699002-330 U6, hsa/mmu/rno miRCURY LNA™ Detection probe, positive control, 1 nmol, 5`-fluorescein and 3`-fluorescein labeled
699002-340 U6, hsa/mmu/rno miRCURY LNA™ Detection probe, positive control, 1 nmol, 5`-DIG labeled
699002-350 U6, hsa/mmu/rno miRCURY LNA™ Detection probe, positive control, 1 nmol, 3`-DIG labeled
699002-360 U6, hsa/mmu/rno miRCURY LNA™ Detection probe, positive control, 1 nmol, 5`-DIG and 3`-DIG labeled
699002-370 U6, hsa/mmu/rno miRCURY LNA™ Detection probe, positive control, 1 nmol, 5`-biotin labeled
699003-300 Sense miR-159 miRCURY LNA™ Detection probe, negative control, 1 nmol, ready to label
699003-310 Sense miR-159 miRCURY LNA™ Detection probe, negative control, 1 nmol, 5`-fluorescein labeled
699003-330 Sense miR-159 miRCURY LNA™ Detection probe, negative control, 1 nmol, 5`-fluorescein and 3`-fluorescein labeled
699003-340 Sense miR-159 miRCURY LNA™ Detection probe, negative control, 1 nmol, 5`-DIG labeled
699003-350 Sense miR-159 miRCURY LNA™ Detection probe, negative control, 1 nmol, 3`-DIG labeled
699003-360 Sense miR-159 miRCURY LNA™ Detection probe, negative control, 1 nmol, 5`-DIG and 3`-DIG labeled
699003-370 Sense miR-159 miRCURY LNA™ Detection probe, negative control, 1 nmol, 5`-biotin labeled
699004-300 Scramble-miR miRCURY LNA™ Detection probe, negative control, 1 nmol, ready to label
699004-310 Scramble-miR miRCURY LNA™ Detection probe, negative control, 1 nmol, 5`-fluorescein labeled
699004-330 Scramble-miR miRCURY LNA™ Detection probe, negative control, 1 nmol, 5`-fluorescein and 3`-fluorescein labeled
699004-340 Scramble-miR miRCURY LNA™ Detection probe, negative control, 1 nmol, 5`-DIG labeled
699004-350 Scramble-miR miRCURY LNA™ Detection probe, negative control, 1 nmol, 3`-DIG labeled
699004-360 Scramble-miR miRCURY LNA™ Detection probe, negative control, 1 nmol, 5`-DIG and 3`-DIG labeled
699004-370 Scramble-miR miRCURY LNA™ Detection probe, negative control, 1 nmol, 5`-biotin labeled
 
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