microRNA (miRNA) Array Spike-in kit from Exiqon

Can be used as a control for microarray labeling and hybridization steps
Simplifies the process of scanner calibration
Can be used as a control for data normalization
An efficient tool in assessments of inter-array variance and intra-array variability

Features

These kits contain synthetic microRNAs that can be used to assess the quality of your microarray data. Add the spike-in microRNAs to your RNA sample before the labeling reaction. Then use the signals from the corresponding array capture probes:

as a control for the labeling reaction and hybridization
to calibrate/adjust scanner settings between channels
as a control for the data normalization procedure
to estimate the variance of replicated measurements within arrays
to assess technical variability between different parts of the array

In addition, by adding one set of spike-in microRNAs before the labeling reaction and another pre-labeled set after the labeling reaction, both the labeling and hybridization efficiencies can be assessed in the same experiment.

Coverage

Two sets of synthetic spike-in microRNAs are available:

Spike-in microRNA Kit contains 10 HPLC-purified, RNase-free synthetic microRNAs that can be detected on all miRCURY LNA™ microRNA Arrays by specifically designed capture probes. This spike-in microRNA kit is included in the miRCURY LNA™ microRNA Array v11 - Other species products.
Spike-in microRNA Kit v2 contains 52 HPLC-purified, RNase-free synthetic microRNAs designed for use with our human, mouse and rat array v10, v11 and 5th gen and with our Other Species Array v11. Note, this kit is not suitable for microRNA profiling in plants. This spike-inmiRNA kit is supplied with the miRCURY LNA™ microRNA Array, 5th gen, hsa, mmu, rno.

The spike-in microRNAs from both kits are provided at concentrations comparable to endogenous microRNA levels and do not cross-react with endogenous human, mouse or rat microRNAs.

See how we compare to other microRNA microarrays

Signal intensity range

The synthetic microRNAs from both kits are provided at concentrations spanning the whole intensity range of microRNAs in most tissue samples. Figure 1 shows the positions of the spike-ins in 250 ng total RNA.

Figure 1

Data quality is easily assessed using the spike-in microRNA kits. (Click to learn more)

“Even microRNAs with a high level of similarity could be distinguished well using these arrays”

Dr. Roman-Ulrich Müller

“The results show some differentially regulated miRNAs and a nice overlap of expression regarding miRNAs encoded by a common primary transcript.”

Dr. Roman-Ulrich Müller, Zentrale Klinishe Forschung, Uniklinik Freiburg.

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“the miRCURY kit is really easy to use and we obtained good quality data”

Dr Shane Murray is a plant genomics expert at the Centre for Proteomic and Genomic Research (CPGR) in Cape Town, South Africa. The CPGR is a modern world class, high throughput biology research facility that provides state-of-the-art analytical services and technical expertise in the genomics and proteomics sectors.

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Bali Muralidhar is working in Dr Nick Coleman's group at the Medical Research Council Cancer Cell Unit in Cambridge, UK. The group is investigating novel approaches to cancer diagnosis. This involves two main areas:

The development of novel markers for improved screening for cervical cancer and colorectal cancer.
The mechanisms of cervical neoplastic progression.

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"The superior detection sensitivity eliminates the need for RNA size selection”

"More answers than questions when it comes to data analysis and interpretation."

Prof Martina Muckenthaler and Prof Matthias Hentze, University of Heidelberg and EMBL, Germany

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miRCURY LNA™ microRNA Array Spike-in Kits

Features

Coverage

Signal intensity range