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miRCURY LNA™ microRNA Array Spike-in Kits

Improve the quality of your miRCURY LNA™ microRNA Array data with these sets of synthetic spike-in microRNAs.

  • Can be used as a control for microarray labeling and hybridization steps
  • Simplifies the process of scanner calibration
  • Can be used as a control for data normalization
  • An efficient tool in assessments of inter-array variance and intra-array variability


 

Features

The kit contains synthetic microRNAs that can be used to assess the quality of your microarray data. Add the spike-in microRNAs to your RNA sample before the labeling reaction. Then use the signals from the corresponding array capture probes:
  • as a control for the labeling reaction and hybridization
  • to calibrate/adjust scanner settings between channels
  • as a control for the data normalization procedure
  • to estimate the variance of replicated measurements within arrays
  • to assess technical variability between different parts of the array
In addition, by adding one set of spike-in microRNAs before the labeling reaction and another pre-labeled set after the labeling reaction, both the labeling and hybridization efficiencies can be assessed in the same experiment.

Coverage

The following set of synthetic spike-in microRNAs is available:
  • Spike-in microRNA Kit v2 contains 52 HPLC-purified, RNase-free synthetic microRNAs designed for use with our human, mouse and rat array 7th gen. Note, this kit is not suitable for microRNA profiling in plants. This spike-in miRNA kit is supplied with the miRCURY LNA™ microRNA Array, 7th gen, hsa, mmu, rno.
The spike-in microRNAs are provided at concentrations comparable to endogenous microRNA levels and do not cross-react with endogenous human, mouse or rat microRNAs.


Signal intensity range

The synthetic microRNAs from both kits are provided at concentrations spanning the whole intensity range of microRNAs in most tissue samples. Figure 1 shows the positions of the spike-ins in 250 ng total RNA.

Figure 1    Easy assessment of data quality  
Data quality is easily assessed using the spike-in microRNA kits. (Click to learn more)   
Roman-Ulrich Müller

The Role of MicroRNAs in Kidney Development


"Even microRNAs with a high level of similarity could be distinguished well using these arrays. The results show some differentially regulated miRNAs and a nice overlap of expression regarding miRNAs encoded by a common primary transcript."

Dr. Roman-Ulrich Müller, Zentrale Klinishe Forschung, Uniklinik Freiburg.

Read full story...

Shane Murray

Molecular Mechanisms of Stress Resistance


"The miRCURY kit is really easy to use and we obtained good quality data."

Dr. Shane Murray is a plant genomics expert at the Centre for Proteomic and Genomic Research (CPGR) in Cape Town, South Africa. The CPGR is a modern world class, high throughput biology research facility that provides state-of-the-art analytical services and technical expertise in the genomics and proteomics sectors.

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Bali Muralidhar

MicroRNA Profiling in Cervical Neoplasms


Bali Muralidhar is working in Dr Nick Coleman's group at the Medical Research Council Cancer Cell Unit in Cambridge, UK. The group is investigating novel approaches to cancer diagnosis. This involves two main areas:

  1. The development of novel markers for improved screening for cervical cancer and colorectal cancer.
  2. The mechanisms of cervical neoplastic progression.
Read full story...

Martina Muckenthaler

The Role of MicroRNA in Homeostasis


"The superior detection sensitivity eliminates the need for RNA size selection. More answers than questions when it comes to data analysis and interpretation."

Prof Martina Muckenthaler and Prof Matthias Hentze, University of Heidelberg and EMBL, Germany.

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