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miRCURY LNA™ microRNA Power Labeling Kits

Fast and simple labeling of microRNA from animals, plants and viruses for subsequent microRNA microarray expression profiling. NEW: Hi-Power Labeling Kit

BackIna K. Dahlsveen, Ph.D., Product Manager

Simple two-step protocol for RNA labeling – no small RNA enrichment required
New Hi-Power labeling kit nearly doubles signal intensities – use substantially less sample
Consistent and reliable results
Uniform labeling
Compatible with all common microarray scanners

Fast and convenient RNA labeling

The miRCURY LNA™ microRNA Power Labeling Kits provide a fast and simple method for fluorescent labeling of total RNA samples from animals, plants and viruses. All small RNAs are uniformly labeled.

The kits are the perfect complement to Exiqon’s highly sensitive and specific miRCURY LNA™ microRNA Arrays. Hybridize the labeled RNA directly to the arrays without additional microRNA enrichments or other time-consuming handling steps.

The miRCURY LNA™ microRNA Power Labeling Kits use a simple and fast two-step protocol (Figure 1):

5’-phosphates are removed from the microRNA termini using Calf Intestinal Alkaline Phosphatase (CIP).
A fluorescent label is enzymatically attached to the 3’-end of the microRNAs in the total RNA sample. After an enzyme inactivation step, the sample is ready for hybridization.

The dyes used (Hy3™ and Hy5™) are spectrally equivalent to the well-known Cy3™ and Cy5™ fluorophores.

A kit for every application

Two different labeling kits are available:

miRCURY LNA™ microRNA Power Labeling Kit – This is our well-known RNA labeling kit. It combines ease-of-use with high performance uniform labeling.

miRCURY LNA™ microRNA Hi-Power Labeling Kit – This is our new and improved labeling kit. It offers a truly amazing performance with an almost doubling of the signal intensity compared to our standard labeling kit (See the 'Experimental data' tab above). This means that the amount of sample can be reduced by almost half without sacrificing the quality of the microarray data.

Both kits are available for single or dual color labeling of total RNA which make the kits very flexible.

Figure 1

Complete labeling and hybridization procedure. (Click to learn more)

Uniform labeling

As shown in Figure 1, our Power Labeling Kits incorporate only one fluorescent label per microRNA resulting in uniform labeling, independent of the extreme 3’-end sequence of the microRNA.

Hi-Power labeling nearly doubles the signal intensity

Exiqon's new miRCURY LNA™ Hi-Power microRNA Labeling Kit has been substantially improved and now offers almost double signal-to-noise ratios compared to our previous labeling kit (Figure 2). This means that microRNAs which are expressed at very low levels close to background can now be clearly distinguished and accurately detected. Using the Hi-Power labeling kit, it is therefore possible to detect more microRNAs from the same amount of input RNA (Figure 3). As seen in Figure 4, the same number of microRNAs are detected with half the amount of RNA using the Hi-Power labeling kit.

Although there is a dramtic difference in the performance of the labeling kits, the labeling patterns of the kits are very similar (Figure 5). This means that microarray data produced using the Power labeling kit can be reproduced using the miRCURY LNA™ Hi-Power microRNA Labeling Kit.

View more experimental data describing the miRCURY LNA™ microRNA Array system

Figure 1

The labeling kits incorporate only one label per microRNA resulting in uniform labeling. (Click to learn more)

Figure 2

Hi-Power labeling results in double signal intensity. (Click to learn more)

Figure 3

Detect more microRNAs using the Hi-Power labeling kit. (Click to learn more)

Figure 4

Detect the same number of microRNAs using half the RNA input. (Click to learn more)

Figure 5

Excellent correlation between the Hi-Power and Power labeling kits. (Click to learn more)

"Exiqon’s product is impressive. The procedures are easy to understand and very fast".

"the Power Labeling Kit is very fast, thus our samples are labeled and ready for hybridization in just half a day"

Dr. Xin Li, Cancer Research Institute of Southern Medical University in Guangzhou, China

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Showing 1 - 6 of 6 products

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Prod. No.

Product Name

Description

Notes

208033

miRCURY LNA™ microRNA Hi-Power Labeling Kit, Hy5

miRCURY LNA™ microRNA, Hi-Power Labeling Kit , Hy5 (24 rxns)

New product

208034

miRCURY LNA™ microRNA Hi-Power Labeling Kit, Hy3

miRCURY LNA™ microRNA, Hi-Power Labeling Kit , Hy3 (24 rxns)

New product

208035

miRCURY LNA™ microRNA Hi-Power Labeling Kit, Hy3/Hy5

miRCURY LNA™ microRNA, Hi-Power Labeling Kit , Hy3/Hy5 (24 rxns)

New product

208030-A

miRCURY LNA™ microRNA Hy5 Power labeling kit

miRCURY LNA™ microRNA, Hy5 Power labeling kit (24 rxns)

208031-A

miRCURY LNA™ microRNA Hy3 Power labeling kit

miRCURY LNA™ microRNA, Hy3 Power labeling kit (24 rxns)

208032-A

miRCURY LNA™ microRNA Hy3/Hy5 Power labeling kit

miRCURY LNA™ microRNA, Hy3/Hy5 Power labeling kit (24 rxns)

Scientific publications

	Haramati et al. Proc. Natl. Acad. Sci. USA 2010
	Lovén et al. Proc. Natl. Acad. Sci. USA 2010
	Suárez et al. Proc. Natl. Acad. Sci. USA 2008
	List of publications
	MicroRNA research 2008 booklet