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miRCURY LNA™ microRNA Power Labeling Kits

Carsten Alsbo, product Manager Fast and simple labeling of microRNA from animals, plants and viruses for subsequent microRNA microarray expression profiling.


Carsten Alsbo, Ph.D., Product ManagerBack
  • Simple two-step protocol requiring no small RNA enrichment
  • MicroRNA profiles from as low as 30 ng total RNA
  • Consistent reliable results
  • Uniform labeling
  • Compatible with all common microarray scanning equipment

Features

The miRCURY LNA™ microRNA Power Labeling Kits provide a fast and simple method for fluorescent labeling of total RNA samples from animals, plants and viruses. All small RNAs are uniformly labeled.

These kits are the perfect complement to Exiqon’s highly sensitive and specific miRCURY LNA™ microRNA Arrays. The labeled RNA molecules may be hybridized directly to the miRCURY LNA™ arrays, without additional microRNA enrichments or other time-consuming handling steps.
 
Our miRCURY LNA™ microRNA Power Labeling Kits follow a simple and fast two-step protocol. First, 5’-phosphates are removed from the microRNA termini using Calf Intestinal Alkaline Phosphatase (CIP). Second, a fluorescent label is attached enzymatically to the 3’-end of the microRNAs in the total RNA sample. This is followed by an enzyme inactivation step after which the sample is ready for hybridization. See Figure 1 for an overview of the complete labeling and hybridization procedure.

Coverage

miRCURY LNA™ microRNA Labeling Kits for single or dual color labeling of total RNA samples are available. The dyes used (Hy3™ and Hy5™) are spectrally equivalent to the well known Cy3™ and Cy5™ fluorophores.  

See how we compare to other microRNA microarrays

Figure 1 Complete labeling and hybridization procedure
Complete labeling and hybridization procedure. (Click to learn more)

Uniform labeling

As shown in Figure 1, our Power Labeling Kits incorporate only one fluorescent label per microRNA resulting in uniform labeling, independent of the extreme 3’-end sequence of the microRNA.

View more experimental data describing the miRCURY LNA™ microRNA Array system
Figure 1  
Our labeling kits incorporate only one label per microRNA resulting in uniform labeling. (Click to learn more)

Dr. Xin Li "Exiqon’s product is impressive. The procedures are easy to understand and very fast".
"the Power Labeling Kit is very fast, thus our samples are labeled and ready for hybridization in just half a day"
Dr. Xin Li, Cancer Research Institute of Southern Medical University in Guangzhou, China

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