Custom microRNA (miRNA) knockdown probes from Exiqon

Pre-designed inhibitors for specific suppression of all microRNAs in miRBase and our proprietary miRPlus™ sequences
Sophisticated and innovative design
Unmatched high efficacy even for AT-rich microRNA targets
High and uniform potency enables multiplexing at low concentrations with minimal risk of off-target effects
Superior specificity and biological stability for long lasting antisense activity
End-labeling option for easy control of transfection efficiency

Features

Custom miRCURY LNA™ microRNA Inhibitors and Power Inhibitors are available for specific knockdown of your own microRNA or other small RNA sequences. These microRNA inhibitors are generated using Exiqon’s in-house design software applying the same novel sophisticated design algorithms used for our pre-desgined microRNA Inhibitors. This ensures the optimal combination of length, sequence and LNA™ positioning in the custom microRNA inhibitor. Moreover, they are designed to have excellent sequence specificity and biological stability, while keeping the self-annealing properties to a minimum.

The miRCURY LNA™ microRNA Power Inhibitors have phosphorothioate (PS) backbones that stabilize the inhibitor. We therefore recommend these inhibitors in difficult applications where:

the transfection efficiency is limiting (primary cells, cells growing in suspension)
the microRNA target is highly expressed
the phenotypic readout that takes place >72 h after transfection (cell differentiation)
regular microRNA inhibitors fail

Coverage

Our custom microRNA inhibitors and Power inhibitors are delivered as ready-to-label or as 3' or 5' pre-labeled fluorescein (6-FAM) oligonucleotides. Requests for alternative labels please contact us. All custom microRNA inhibitors are HPLC-purified and desalted and delivered in tubes containing 5 nmol dried-down oligonucleotide ready for transfection or electroporation using standard techniques

Before submitting your order, please check if a pre-designed microRNA inhibitor targeting your sequence is available. Pre-designed miRCURY LNA™ microRNA microRNA Inhibitors and Power Inhibitors are available for all microRNA sequences annotated in miRBase (v.13.0).

View experimental data on the miRCURY LNA™ microRNA Inhibitors and Power Inhibitors

"...thanks to the LNA™ microRNA inhibitor screen, we have discovered several new miRNAs playing a major role in the regulation of the proliferation/differentiation balance in prostate cells."

Dr. Xavier Gidrol directs the Biomics Laboratory at the Institute of Life Science Research and Technologies in Grenoble, France. They use microfluidics, micromanufacturing and MEMS to study the impact of genetic and micro-environmental determinants on carcinogenesis, at the scale of a few or even single cells. Xavier has used minute cell microarrays to perform high throughput and high content screening with our human library of microRNA inhibitors in primary prostate cancer cells extracted from patients.

Read full story...

"Previously, we had tried other inhibitor designs but these gave inconclusive results"

Amy Hansen is a Ph.D student at the University College London. She works in the Cancer Research UK Viral Oncology Group lead by Professor Chris Boshoff, Director of the UCL Cancer Institute.

Read full story...

"LNA microRNA antisense inhibitors are efficient and specific with long lasting effects"

Dr. Annick Harel-Bellan (AHB) is Directeur de Recherche at the Institut Andre Lwoff in Paris. She heads a group working on epigenetics and cancer (Laboratoire Epigenetique et Cancer). Dr. Anna Polesskaya (AP) is a senior scientist and longstanding member of this group

Read full story...