 MicroRNAs and diabetes
"We found that Exiqon's LNA™ microRNA microarrays were versatile and easy to use"
Himawan Harryanto at the University of Adelaide, Australia, studies the effect of fetal growth restriction on the development of diabetes in adult life. He has used Exiqon's microRNA microarray system to profile microRNAs in the pancreas.
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  MicroRNAs in heart disease
“The LNA™-inhibitors from Exiqon just work. ”
Stefanie Dimmeler, Professor and group leader, and Reinier Boon, Post Doctoral researcher, work in the Institute for Cardiovascular Regeneration at the Goethe University Frankfurt, Germany. Here they study the basic mechanisms underlying cardiovascular disease and vessel growth with the aim to develop new therapies to improve treatment of cardiovascular diseases.
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 MicroRNAs in Skin
"Exiqon is one of the leading suppliers for miRNA-related products"
Dr. Liming Luan at Vanderbilt University Medical Center in Nashville studies the role of microRNAs in normal and pathological conditions of the skin.
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 High content microRNA inhibitor screening with cell arrays
"...thanks to the LNA™ microRNA inhibitor screen, we have discovered several new miRNAs playing a major role in the regulation of the proliferation/differentiation balance in prostate cells." Dr. Xavier Gidrol directs the Biomics Laboratory at the Institute of Life Science Research and Technologies in Grenoble, France. They use microfluidics, micromanufacturing and MEMS to study the impact of genetic and micro-environmental determinants on carcinogenesis, at the scale of a few or even single cells. Xavier has used minute cell microarrays to perform high throughput and high content screening with our human library of microRNA inhibitors in primary prostate cancer cells extracted from patients.
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 Viral microRNAs Involved in Cell Reprogramming
"Previously, we had tried other inhibitor designs but these gave inconclusive results"
Amy Hansen is a Ph.D student at the University College London. She works in the Cancer Research UK Viral Oncology Group lead by Professor Chris Boshoff, Director of the UCL Cancer Institute.
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 MicroRNAs in Hypoxia
"I was intrigued by this interesting technology, which is very different from other available technologies."
Dr. Mircea Ivan at Indiana University School of Medicine studies the role of microRNAs in hypoxia. Here he describes why his lab chose Exiqon's detection probes.
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  Changes in Gene Expression in Response to Starvation
“The miRNA Profiling Services at Exiqon has performed the technical aspects of the analysis, a task which requires time and specialized knowledge. This has allowed us to focus on the quality of biological aspects of the experiment.”
Dr Tom Hamborg Nielsen, Associate Professor and group leader, and Dr. Maria Lundmark, Post Doctoral researcher, both work at the Plant Molecular Biology Laboratory at Copenhagen University, Denmark. They study how plants respond to phosphate starvation by changing their gene expression.
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  MicroRNAs and Muscle Cell Differentiation
"LNA microRNA antisense inhibitors are efficient and specific with long lasting effects"
Dr. Annick Harel-Bellan (AHB) is Directeur de Recherche at the Institut Andre Lwoff in Paris. She heads a group working on epigenetics and cancer (Laboratoire Epigenetique et Cancer). Dr. Anna Polesskaya (AP) is a senior scientist and longstanding member of this group
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 Quantitation of microRNAs in Tumor Angiogenesis
"the scientists at Exiqon were very, very supportive" Dr. Dorina Veliceasa, from the department of Urology at the Northwestern University Feinberg School of Medicine, discusses the use of the miRCURY LNA™ microRNA PCR system in her investigation of tumor angiogenesis in endothelial cells. Dr. Veliceasa discusses the challenges of qPCR monitoring of microRNA species, including the importance of endogenous controls, and offers advice to other researchers interested in this method of determining microRNA expression levels.
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 The Role of MicroRNA in Early Embryonic Development
“LNA™ technology is superior to any other for the detection of small RNA species” Dr. Parker Antin’s lab at the University of Arizona runs a large scale in situ hybridization database project to determine the expression patterns for all differentially expressed genes in the chicken embryo. Find out what the challenges were with this huge project and how they were overcome. |
 Molecular Mechanisms of Stress Resistance
“the miRCURY kit is really easy to use and we obtained good quality data” Dr. Shane Murray is a plant genomics expert at the Centre for Proteomic and Genomic Research (CPGR) in Cape Town, South Africa. The CPGR is a modern world class, high throughput biology research facility that provides state-of-the-art analytical services and technical expertise in the genomics and proteomics sectors. |
 MicroRNAs in Muscle Cell Differentiation
“Following the success of LNA™ probes as demonstrated in Plasterk’s lab, we decided to take the same approach” Dr. Dylan Sweetman is working in Dr. Andrea Münsterberg’s group at the University of East Anglia, School of Biological Sciences. The group investigates cellular and molecular mechanisms that underlie embryonic development. |
MicroRNA Profiling in Cervical Neoplasms
Bali Muralidhar is working in Dr Nick Coleman's group at the Medical Research Council Cancer Cell Unit in Cambridge, UK. The group is investigating novel approaches to cancer diagnosis. This involves two main areas: - The development of novel markers for improved screening for cervical cancer and colorectal cancer.
- The mechanisms of cervical neoplastic progression.
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 Studying MicroRNAs in Acute Leukemia
"We chose to use miRCURY LNA™ knockdown probes as we had previous experience with using LNA probes for in situ hybridization with great success".
Amanda Dixon-McIver is finishing her Ph.D. under the supervision of Dr. Silvana Debernardi, in the Medical Oncology Laboratory headed by Prof. Bryan Young in the Institute of Cancer, Barts Hospital, in London, UK.
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 Nasopharyngeal Cancer Development
"Exiqon’s product is impressive. The procedures are easy to understand and very fast".
"the Power Labeling Kit is very fast, thus our samples are labeled and ready for hybridization in just half a day"
Dr. Xin Li, Cancer Research Institute of Southern Medical University in Guangzhou, China |
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 MicroRNA Involvment in Hormone Action
"The miRCURY™ LNA microRNA PCR system is easy to use and provides high sensitivity and reproducibility.”
"It makes my validation faster, easier and more quantitative"
Scientist Louisa Cheung, Karolinska Institute, Sweden
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 The Role of MicroRNAs in Kidney Development
“Even microRNAs with a high level of similarity could be distinguished well using these arrays”
“The results show some differentially regulated miRNAs and a nice overlap of expression regarding miRNAs encoded by a common primary transcript.”
Dr. Roman-Ulrich Müller, Zentrale Klinishe Forschung, Uniklinik Freiburg.
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 MicroRNAs and Homeostasis
"The superior detection sensitivity eliminates the need for RNA size selection”
"More answers than questions when it comes to data analysis and interpretation."
Prof. Martina Muckenthaler and Prof. Matthias Hentze, University of Heidelberg and EMBL, Germany
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 MicroRNA Involvment in Embryo Development
“We got some beautiful data and spectacular images”
"We generated a complete catalogue of images showing the temporal and spatial expression patterns of 115 conserved microRNAs in zebrafish embryos."
Prof. Ronald H. A. Plasterk, Hubrecht Laboratory, The Netherlands
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