miRCURY LNA™ microRNA Detection Probes for in situ hybridization  LNA™-enhanced detection probes for ultra-sensitive and specific in situ hybridization of microRNAs.
Ina K. Dahlsveen, Ph.D., Product Manager Back | |
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- Superior sensitivity and specificity
- Detection of low abundance microRNAs
- Probes available for all known microRNAs
- A wide selection of labels available
- Fully developed protocols
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Features The miRCURY LNA™ microRNA Detection Probes are designed with optimal LNA™ positioning to achieve high sequence specificity, low secondary structure and minimal self-annealing. The melting temperatures ( T m) of the probe:target duplexes are designed to be within the 70-85ºC range.
Coverage Pre-designed miRCURY LNA™ microRNA Detection Probes for in situ hybridization are available for all known microRNAs in invertebrates, vertebrates and plants as registered and annotated in the microRNA registry (miRBase) at the Wellcome Trust Sanger Institute. Our standard probes are available with a selection of 3’ and 5’ labels (DIG*, biotin, fluorescein, amino). “Ready-to-label” probes designed for custom labeling are also available.
Note Whenever an optimal signal to noise ratio is required, e.g. to detect low abundance microRNAs, we recommend the combined 5’ and 3’ double DIG*-labeled miRCURY LNA™ microRNA detection probes. These are the most sensitive detection probes available due to a cooperative effect of the two DIG* labels. To order Double DIG* miRCURY LNA™ microRNA Detection Probes, please contact us.
Custom miRCURY LNA™ microRNA Detection Probes are available for your own microRNAs and other small RNAs, including pre-microRNAs. Positive and negative control probes are also available.
*Licensed from Roche Diagnostics GmbH
To find a particular miRCURY LNA™ microRNA Detection Probe, please use the search function below.
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Specificity and sensitivity The high affinity and discrimination of the miRCURY LNA™ microRNA Detection Probes result in a sensitive detection of microRNAs. Specific in situ detection of microRNA is possible in whole mounts, thin sections, single cells, frozen samples and in formalin-fixed, paraffin-embedded tissue sections including archived samples (Figures 1 and 2).
When looking for low abundance microRNAs, or in other situations where high sensitivity is of paramount importance, we recommend using our combined 5’ and 3’ double DIG* labeled miRCURY LNA™ miRNA detection probes. These are the most sensitive detection probes available due to a synergistic effect of the two DIG* labels. The up to 10-fold higher signal to noise ratio makes the detection of even low expressed targets feasible (Figure 3). To order Double DIG* miRCURY LNA™ microRNA detection probes, please contact us .
miRCURY LNA™ Detection probes have been used successfully in both animal and plant species. A number of peer-reviewed publications have demonstrated the excellent performance of these probes by showing highly specific microRNA expression pattern in different tissues and cells (Figures 4 and 5).
Further reading View our gallery of nearly 1000 in situ images.
More images are available at GEISHA, a project hosted by the University of Arizona, aimed at identifying differentially expressed genes in chicken embryos.
Customer testimonials are available in our Application stories section.
Scientific videos
*Licensed from Roche Diagnostics GmbH |
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Figure 1
Visualization of a liver specific microRNA. (Click to learn more)
Figure 2
microRNA detection in brain FFPE sections. (Click to learn more)
Figure 3
Double (A) and single (B) DIG* labeled probes compared. (Click to learn more)
Figure 4
Tissue specific detection of microRNAs in zebrafish. (Click to learn more)
Figure 5
Detection of muscle specific microRNAs in chick embryos. (Click to learn more) |
 "I was intrigued by this interesting technology, which is very different from other available technologies."
Dr. Mircea Ivan at Indiana University School of Medicine studies the role of microRNAs in hypoxia. Here he describes why his lab chose Exiqon's detection probes.
Read full story... |
 “We got some beautiful data and spectacular images”
"We generated a complete catalogue of images showing the temporal and spatial expression patterns of 115 conserved microRNAs in zebrafish embryos."
Prof Ronald H. A. Plasterk, Hubrecht Laboratory, The Netherlands
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 “Following the success of LNA™ probes as demonstrated in Plasterk’s lab, we decided to take the same approach” Dr. Dylan Sweetman is working in Dr. Andrea Münsterberg’s group at the University of East Anglia, School of Biological Sciences. The group investigates cellular and molecular mechanisms that underlie embryonic development. |
 “LNA™ technology is superior to any other for the detection of small RNA species” Dr. Parker Antin’s lab at the University of Arizona runs a large scale in situ hybridization database project to determine the expression patterns for all differentially expressed genes in the chicken embryo. Find out what the challenges were with this huge project and how they were overcome. |
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Note:
Searches using “miRNA name” may return products with a miRNA name from a different species (typically hsa-miR-xxxx) with an identical target sequence. To see all microRNAs targeted by the detection probe, click on the product and go to the “Targeted microRNAs” tab.
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Search tips:
You can search our products database in three ways. The easiest procedure is simply using the name of the microRNA under study:
Enter the mature microRNA name (e.g. hsa-miR-142-3p). It is also possible to search using parts of microRNA names, e.g., "hsa-let" to see all probes related to the hsa-let7 microRNA family.
If no results are returned please check that the entered information is accurate or entered in the correct field. If the problem persists please contact our technical support.
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