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cDNA Synthesis and SYBR® Green Master Mix Kits

The ExiLERATE cDNA synthesis kit enables optimal reverse transcription of all mRNA and lncRNA in one tube. The thermostable M-MuLV RT operates at elevated temperatures to ensure a high yield of full length cDNA even from transcripts with strong secondary structures. For unrivalled sensitivity and specificity, combine with the ExiLERATE SYBR® Green master mix containing Hot Start Taq DNA Polymerase.

  • Best in class sensitivity and specificity
  • Very high reproducibility ensures consistent results
  • High yield of full length cDNA
  • Fast – cDNA synthesis completed in just 30 minutes
  • Unique thermostable RT efficiently transcribes any target, even those with high GC content
  • Master Mix optimized to eliminate non specific amplification
  • Optimized reagents for use with the ExiLERATE LNA™ qPCR primer sets

ExiLERATE LNA™ qPCR, cDNA synthesis kit

The ExiLERATE cDNA synthesis kit contains all reagents required to do 50x 20µL universal cDNA synthesis reactions. The kit is based on a modified M-MuLV RT allowing reverse transcription at high temperatures and with no RNase H activity, ensuring efficient reverse transcription of targets with strong secondary structures. The cDNA uses a universal RT approach ensuring few pipetting steps and consumption of RNA is kept low. The reaction is completed in just 30 minutes ensuring fast results.

ExiLERATE LNA™ qPCR, SYBR® Green master mix

The ExiLERATE LNA™ qPCR SYBR® Green master mix is a high-performance PCR master mix specifically designed for Exiqon’s ExiLERATE LNA™ qPCR system. The Master Mix is optimized to eliminate non specific amplification. The kit includes a GAPDH assay for use as potential endogenous control. The ExiLERATE LNA™ qPCR SYBR® Green Master mix is available in two sizes. The 2.5 ml kit contains enough reagents for 500 10µl PCR reactions. The 10ml kit contains enough reagents for 2000 10µl reactions. The master mix is ready-to-use and just need to be mixed with primers and template.



Unmatched sensitivity

The exceptional sensitivity of the ExiLERATE LNA™ qPCR primer sets is achieved by combining the following features:
  • Optimized design algorithm using LNA™ enhanced and Tm normalized primers
  • Universal RT with the ExiLERATE LNA™ qPCR cDNA synthesis kit
This combination enables accurate and reliable quantification of abundant mRNAs and lncRNAs from as little as 1 pg of total RNA input in the initial first-strand cDNA synthesis and efficient detection of most RNAs from 0.1 ng total RNA (Figure 1). High sensitivity is crucial for the detection of low abundance targets, such as lncRNA.

Excellent specificity

The incorporation of LNA™ in the PCR amplification primers (forward and reverse) facilitates the design of highly specific assays that can distinguish between RNA sequences that differ by only one nucleotide. In addition, the primers can be targeted freely over the target sequence despite AT content, due to the incorporation of LNA™ in the primers.

The use of ExiLERATE LNA™ qPCR SYBR® Green Master Mix, makes melt curve analysis possible, so that the specificity of an assay can be evaluated (Figure 2).

Superior performance – success guaranteed

The ExiLERATE LNA™ qPCR system offers superior performance compared to all major competitors, in terms of sensitivity (Figure 5). When testing the performance of assays designed to ten randomly selected mRNA transcripts, the ExiLERATE LNA™ qPCR assays were able to detect all targets at 1 ng total RNA input, a success rate far superior to all major competitors (Figure 6).
Enhanced by LNA™ – Success guaranteed

Note

The ExiLERATE LNA™ qPCR primers have been optimized for use with the ExiLERATE LNA™ qPCR, cDNA synthesis kit and SYBR® Green master mix kit. Use of other reagents will affect the quality of the results.

ExiLERATE guarantee

If you are unhappy with the performance of an ExiLERATE LNA™ qPCR assay, our technical support team will work with you to identify the issue and if required replace with a different assay for the same target at no extra cost. Data from specific customer experiments may be required to evaluate assay performance e.g. use of the GAPDH primer set and/or independent evidence for transcript expression. Any problem should be reported within 3 months of delivery. ExiLERATE LNA™ qPCR primers must be used with the ExiLERATE LNA™ qPCR, cDNA synthesis kit and SYBR® Green master mix kit. Exiqon reserves the right to modify the terms of this guarantee at any time.

Figure 1 ExiLERATE LNA™ qPCR system offers excellent sensitivity down to 0.1 ng total RNA
Excellent sensitivity. (Click to learn more)


Figure 2 Human GAPDH was amplified from a total RNA sample
Beautiful amplification and melting curves (human GAPDH). (Click to learn more)


Figure 3 Mouse GAPDH was amplified from a total RNA sample
Beautiful amplification and melting curves (mouse GAPDH). (Click to learn more)


Figure 4 Rat GAPDH was amplified from a total RNA sample
Beautiful amplification and melting curves (rat GAPDH). (Click to learn more)


Figure 5 ExiLERATE LNA™ qPCR sensitivity exceeds all major competitors
ExiLERATE LNA™ qPCR sensitivity exceeds all major competitors. (Click to learn more)


Figure 6 ExiLERATE LNA™ qPCR assays outperform all major competitors
ExiLERATE LNA™ qPCR assays outperform all major competitors. (Click to learn more)


NOTICE TO PURCHASER: LIMITED LICENSE Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser’s own internal research. No other patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. SYBR® Green is a trademark of Invitrogen.
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