LNA™ Technology Locked nucleic acid (LNA™) nucleosides are a class of nucleic acid analogues in which the ribose ring is “locked” by a methylene bridge connecting the 2’-O atom with the 4’-C atom (see structure below). LNA™ nucleosides containing the six common nucleobases (T, C, G, A, U and mC) that appear in DNA and RNA are able to form base pairs with their complementary nucleosides according to the standard Watson-Crick base pairing rules.
However, by “locking” the molecule with the methylene bridge the LNA™ is constrained in the ideal conformation for Watson-Crick binding. LNA™ therefore makes the pairing with a complementary nucleotide strand more rapid and increases the stability of the resulting duplex. LNA™ nucleosides give rise to increased thermal stability and discriminative power of duplexes resulting in various unique features.
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LNA™ applications: Compatible with: - DNAzymes
- siRNA
- PCR primers
- DNA, RNA and other monomers
- Any dye, quencher or linker
- Standard phosphoramidite chemistry
- Aqueous solutions
- Most enzymes used in molecular biology
Facilitates: - Increased target specificity
- Single base discrimination
- Strand invasion
- Tm normalization
- Shorter probes (even as short as 7-mers)
- Predictable melting & hybridization behavior
For further information, view the material under Documentation to the right.
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