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| microRNA Knockdown |
miRCURY™ LNA Knockdown probes: Confirm or determine miRNA function.
Key benefits:
- Effective antisense inhibition of miRNA
- Highly specific miRNA knockdown
- Minimal cytotoxicity
- Compatible with standard cell transfection methods
- Increased biological stability
Exiqon's miRCURY™ LNA Knockdown technology enables sequence-specific inhibition of mature miRNAs in vitro and in vivo. Depletion of miRNAs can be judged by the decrease in hybridization signals in Northern blots as LNA-miRNA hybrids are thermally stable in denaturing gels.
Exiqon uses in-house bioinformatics expertise to optimally design miRCURY™ LNA Knockdown probes for specific knockdown of miRNAs.
miRCURY™ LNA Knockdown probes have high potency, without compromising specificity. Effective knockdown of miRNA is achieved with standard or low concentrations of miRCURY™ LNA Knockdown probe. However, this knockdown is reduced significantly with the presence of a mismatch between the probe and the target. Our data demonstrates that the presence of two mismatches between a miRCURY™ LNA Knockdown probe and its target is sufficient to completely eliminate inhibition. This is important within the cell, where closely related miRs may be present and the knockdown needs to be as specific as possible.
miRCURY™ LNA Knockdown probes have also been demonstrated to be more effective than standard methods used in miRNA knockdown, such as DNA-based probes and those based on 2’-O-methyl chemistry.
The inclusion of LNA™ in Exiqon’s miRCURY™ LNA Knockdown probes provides improved resistance to cellular nucleases, thus providing more lengthy periods over which to conduct your knockdown studies.
Pre-designed miRCURY™ in situ knockdown probes are available for all known miRNAs in invertebrates, vertebrates and plants, as registered and annotated in the miRNA Registry at The Wellcome Trust Sanger Institute. Please click here to view prices and labeling options.
The following control miRCURY™ knockdown probes are available: sense-miR-159 and scramble-miR. These probes have the same length and LNA content as the miRCURY™ Knockdown probes and they bear no homology to any known miRNA or mRNA sequence in human, mouse and rat. click here to view prices and labeling options.
Custom miRCURY™ knockdown probes are available for your own miRNAs. Please enquire.
All probes are available as ready-to-label as well as 3´ or 5´ prelabeled probes.
For further information, please view the material in the Documentation Box to the right.
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| Publications
Nature Aplication Notes
Technical notes: |
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.gif "Comparison of miRCURY™ LNA Knockdown probes")
