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Pre-validated LNA™ FISH Probes

Carsten Alsbo, Product Manager Fast, sensitive and specific detection of chromosomal DNA sequences with pre-validated LNA™-enhanced probes.


Carsten Alsbo, Ph.D., Product Manager
  • Pre-validated probes
  • Short hybridization time (< 1h)
  • Works on both fixed cells and chromosome spreads
  • Multiplex capability
  • Available with different labels

Features

Although fluorescence in situ hybridization (FISH) has proven useful in cytogenetics, it has traditionally been a time-consuming method with limitations in sensitivity and resolution. Our high-affinity LNA™ FISH Probes offer fast, sensitive and specific detection of chromosomal DNA.

Coverage

Pre-validated LNA™-enhanced FISH probes targeting human classical satellite-2 repeats, human telomeres and human centromere alpha-satellite sequences are available. These probes are available with 5’-biotin or 5’-fluorescein labels.

For custom-made LNA™-probes targeting other chromosomal sequences, please contact Exiqon.

The high affinity of LNA™-enhanced probes for their complementary sequences make them ideal for chromosomal FISH. These probes give strong signals even after hybridization periods of less than one hour.

Figure 1
Specific telomere detection using LNA(tm) FISH probes
LNA™ FISH probes targeting telomeric sequences. (Click to learn more) 


Figure 2 Specific telomere detection using LNA(tm) FISH probes
 
LNA™ probes (A) are better than DNA probes (B) at detecting satellite-2 repeats. (Click to learn more) 


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