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Custom LNA™ FISH Probes

Fast, sensitive and specific detection of chromosomal DNA sequences using custom LNA™-enhanced probes.

  • Design your own LNA™ FISH probes or let us do the design for you
  • Short hybridization time (< 1h)
  • Works on both fixed cells and chromosome spreads
  • Multiplex capability
  • Available with different labels


Although fluorescence in situ hybridization (FISH) has proven useful in cytogenetics, it has traditionally been a time-consuming method with limitations in sensitivity and resolution. Our high-affinity LNA™ FISH Probes offer fast, sensitive and specific detection of chromosomal DNA.

Let Exiqon design your probes

We are happy to design LNA™ probes targeting customer-defined targets. Please contact us for more information. Alternatively, design your own probes using the guidelines below.

Design your own probes

Use the guidelines below to design your own FISH probes. In addition, our bioinformatics tools  can be used to simplify the design process. When you are ready to order, just click the "Order this product" button at the bottom of this page.

Probe design

  • Detection probes are typically 20-25 nucleotides in length. However, shorter or longer probes can also be used.
  • Avoid stretches of 3 or more Gs or Cs.
  • Avoid stretches of more than 4 LNA™ bases, except when very short (9-10 nt) oligonucleotides are designed.
  • Avoid LNA™ self-complementarity. LNA™ hybridizes very tightly to other LNA™ residues.
  • Keep the GC-content between 30-60 %.
  • A T m of approximately 75 °C is recommended.
  • No LNA™ bases should be placed in palindromes (G-C base pairs are more critical than A-T base pairs).

The high affinity of LNA™-enhanced probes for their complementary sequences make them ideal for chromosomal FISH. These probes give strong signals even after hybridization periods of less than one hour.

Figure 1
Specific telomere detection using LNA(tm) FISH probes
LNA™ FISH probes targeting telomeric sequences. (Click to learn more)

Figure 2 Specific telomere detection using LNA(tm) FISH probes

LNA™ probes (A) are better than DNA probes (B) at detecting satellite-2 repeats. (Click to learn more)

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