Exiqon
Home Search Contact Print
0 items in basket0
Sign In
 
 
Products Services Applications Resource Center Ordering About Exiqon
microRNA Research
lncRNA Research
mRNA Research
DNA Research
Custom Oligos
Other Reagents
RNA Isolation
Sequencing
Microarray Analysis
Real-time PCR
Northern Blotting
In Situ Hybridization
Functional Analysis
RNA Preparation
Sequencing
Microarray analysis
Real-time PCR
In Situ Hybridization
Antisense
RNA Preparation
Sequencing
Microarray analysis
Real-time PCR
In Situ Hybridization
Antisense
SNP Detection
Sequencing
Microarray Analysis
In Situ Hybridization
LNA Phosphoramidites
A2 Quencher
RNA Isolation Services
microRNA Array Services
microRNA PCR Services
Sequencing Services
microRNA ISH Services
Custom Pharma Service
Isolation
Microarray Analysis
Northern Blotting
Real-time PCR
In Situ Hybridization
Functional Analysis
SNP Detection
RNA
microRNA
RNA
DNA
microRNA
microRNA
mRNA
microRNA
Other RNAs
DNA
microRNA
RNA
DNA
microRNA Resources
mRNA and lncRNA Resources
Documents
Reading rooms
Technology
Oligo design tools
Movies
microRNA
Research Guide
Reading rooms
Application Stories
Bioinformatics and data analysis
What are lncRNAs?
Application Stories
Bioinformatics
Oligo modifications
LNA™
AQ-link
Order microRNA products
Order custom oligos
Distributors
Global Service Providers
General Information
Contact
News
Company profile
History
Management
Board of Directors
Awards & Partnerships
Newsletter
Career
Sign up to Newsletter
Newsletter Back Issues
 

Custom LNA™ FISH Probes

Fast, sensitive and specific detection of chromosomal DNA sequences using custom LNA™-enhanced probes.

  • Design your own LNA™ FISH probes or let us do the design for you
  • Short hybridization time (< 1h)
  • Works on both fixed cells and chromosome spreads
  • Multiplex capability
  • Available with different labels

Features

Although fluorescence in situ hybridization (FISH) has proven useful in cytogenetics, it has traditionally been a time-consuming method with limitations in sensitivity and resolution. Our high-affinity LNA™ FISH Probes offer fast, sensitive and specific detection of chromosomal DNA.

Let Exiqon design your probes

We are happy to design LNA™ probes targeting customer-defined targets. Please contact us for more information. Alternatively, design your own probes using the guidelines below.

Design your own probes

Use the guidelines below to design your own FISH probes. In addition, our bioinformatics tools  can be used to simplify the design process. When you are ready to order, just click the "Order this product" button at the bottom of this page.

Probe design

  • Detection probes are typically 20-25 nucleotides in length. However, shorter or longer probes can also be used.
  • Avoid stretches of 3 or more Gs or Cs.
  • Avoid stretches of more than 4 LNA™ bases, except when very short (9-10 nt) oligonucleotides are designed.
  • Avoid LNA™ self-complementarity. LNA™ hybridizes very tightly to other LNA™ residues.
  • Keep the GC-content between 30-60 %.
  • A T m of approximately 75 °C is recommended.
  • No LNA™ bases should be placed in palindromes (G-C base pairs are more critical than A-T base pairs).



The high affinity of LNA™-enhanced probes for their complementary sequences make them ideal for chromosomal FISH. These probes give strong signals even after hybridization periods of less than one hour.

Figure 1
Specific telomere detection using LNA(tm) FISH probes
LNA™ FISH probes targeting telomeric sequences. (Click to learn more)


Figure 2 Specific telomere detection using LNA(tm) FISH probes

LNA™ probes (A) are better than DNA probes (B) at detecting satellite-2 repeats. (Click to learn more)


 
  Privacy   Sitemap   Legal