Exiqon
Home Search Contact Print
0 items in basket0
Sign In
 
 
Products Services Applications Resource Center Ordering About Exiqon
microRNA Research
lncRNA Research
mRNA Research
DNA Research
Custom Oligos
Other Reagents
RNA Isolation
Sequencing
Microarray Analysis
Real-time PCR
Northern Blotting
In Situ Hybridization
Functional Analysis
RNA Preparation
Sequencing
Microarray analysis
Real-time PCR
In Situ Hybridization
Antisense
RNA Preparation
Sequencing
Microarray analysis
Real-time PCR
In Situ Hybridization
Antisense
SNP Detection
Sequencing
Microarray Analysis
In Situ Hybridization
LNA Phosphoramidites
A2 Quencher
RNA Isolation Services
microRNA Array Services
microRNA PCR Services
Sequencing Services
microRNA ISH Services
Custom Pharma Service
Isolation
Microarray Analysis
Northern Blotting
Real-time PCR
In Situ Hybridization
Functional Analysis
SNP Detection
RNA
microRNA
RNA
DNA
microRNA
microRNA
mRNA
microRNA
Other RNAs
DNA
microRNA
RNA
DNA
microRNA Resources
mRNA and lncRNA Resources
Documents
Reading rooms
Technology
Oligo design tools
Movies
microRNA
Research Guide
Reading rooms
Application Stories
Bioinformatics and data analysis
What are lncRNAs?
Application Stories
Bioinformatics
Oligo modifications
LNA™
AQ-link
Order microRNA products
Order custom oligos
Distributors
Global Service Providers
General Information
Contact
News
Company profile
History
Management
Board of Directors
Awards & Partnerships
Newsletter
Career
Sign up to Newsletter
Newsletter Back Issues
 

Custom microRNA LNA™ PCR primer sets

Design custom LNA™-enhanced microRNA primer sets for your own microRNA. Use our web tool to design super sensitive primers compatible with the miRCURY LNA™ Universal RT microRNA PCR System.

  • Design LNA™-enhanced microRNA PCR primer sets for any microRNA in minutes using our online software tool
  • Fully compatible with the miRCURY LNA™ Universal RT microRNA PCR System

Features

Design LNA™-enhanced qPCR primer sets for any microRNA using Exiqon’s online Custom miRCURY LNA™ Universal RT microRNA PCR Assay design software .

The designed primers are compatible with the miRCURY LNA™ Universal RT microRNA PCR System. Please make sure that pre-designed validated primer sets are not available for your microRNA before proceeding with custom primer design.

Coverage

PCR primer sets designed with Exiqon’s online software tool are provided in amounts sufficient for 100 PCR reactions using the miRCURY LNA™ Universal RT microRNA PCR System.

Ordering

The primer sets can be ordered directly online or by contacting us with the reference number received from the design software. Prices can be found by placing the designs in the shopping basket.

The design process

The Custom miRCURY LNA™ Universal RT microRNA PCR Assay design software is ideal for designing highly sensitive and specific LNA™-enhanced PCR primer sets for any microRNA not available as a pre-designed product.

In a process which typically takes about two minutes, an advanced algorithm evaluates approximately 3,000 primer pair designs based on more than 60 different criteria to find LNA™ optimal primer sets for your microRNA. Note, the design software has been designed for microRNAs but can also be used for other small RNAs (14-27 nt in length).

The design criteria include:
  • Optimization of melting temperatures by varying the LNA™ distribution of the primers to ensure high amplification efficiency and specificity.
  • Calculations and adjustments of self-hybridization and cross-hybridization scores for efficient PCR reactions.
  • Adjustments to avoid potential primer-dimer formation in the PCR reaction.
  • Intelligent positioning of LNA™ bases in the primers based on Exiqon’s vast knowledge of LNA™ oligonucleotide design.
  • miRBase searches to identify potential microRNAs with high sequence similarity. This ensures that the designed primer sets are highly specific for the small RNA for which they were designed.

View experimental data for our standard miRCURY LNA™ Universal RT microRNA PCR Primer Sets



NOTICE TO PURCHASER: DISCLAIMER OF LICENSE No license is conveyed with the purchase of this product under any of US Patents Nos. 5,210,015, 5,487,972, 5,804,375, 5,994,056, 6,171,785, 6,214,979, 5,538,848, 5,723,591, 5,876,930, 6,030,787, and 6,258,569, and corresponding patents outside the United States, or any other patents or patent applications, relating to the 5’ Nuclease and dsDNA-Binding Dye Processes. For further information contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
  Privacy   Sitemap   Legal