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Cancer Focus microRNA PCR Panels

PCR panels containing miRCURY LNA™ Universal RT microRNA PCR assays for microRNAs related to cancer. Panels are available in ready-to-use 96 well plates – just add cDNA and PCR master mix. New v4 panels with optimized assays for improved specificity.

*25% discount on any miRNome, Focus or Custom Pick-&-Mix panels until 27 December

Discount is valid on orders received by 27 December 2016. Order must include microRNA qPCR panels , Universal cDNA Synthesis Kit II (prod no 203301) and ExiLENT SYBR® Green master mix (prod no 203421 or 203403). 25% discount applies to microRNA qPCR panels. Please mention the promo code qPCR-Xmas16 when ordering. Discount may not be combined with any other offer. Discount valid only in regions where Exiqon offers direct sales. In countries where Exiqon uses distributors, please contact your distributor.
  • All relevant oncogenes and/or tumor suppressor microRNAs in one PCR Panel
  • Quick and convenient identification of microRNA biomarker or signatures in cancer samples
  • Profile all microRNAs using less than 20 ng total RNA
  • Sensitivity and specificity for accurate microRNA profiling from FFPE, biopsies and LCM samples
  • Fast and easy - ready-to-use format for direct sample application with a full profile in less than 3 hours
  • High compatibility – 96 and 384 well formats compatible with most real-time PCR instruments

Cancer Focus microRNA PCR Panel

Exiqon’s Cancer Focus microRNA PCR Panel targets 84 human microRNAs related to cancer. These microRNAs have been shown to be important for tumorigenesis and in cancer diagnostics.

These microRNAs can be used as markers for 27 different types of cancer (Table 1). They have been identified from a large biobank containing thousands of FFPE samples as well as from literature data. For a list of references of each microRNA on the panel, please refer to the plate layout file.

The panel is designed for cancer microRNA analysis of cancer progression, recurrence and metastasis and will help researchers to quickly and accurately analyze cancer samples and help identify new microRNA-based biomarkers.

Product coverage

Cancer Focus microRNA PCR Panels are available in:
  • 96 well plate format: 4 sets of 96-well plates with one Cancer Focus Panel (sufficient for 4 samples).
  • 384 well plate format: Eight Cancer Focus Panels delivered in two 384 well plates (sufficient for 8 samples). These plates have been specifically designed for fast and easy manual and multi-pipetting.
Both formats are Ready-to-use with 10µl reactions per well.

The panel contains assays for the following:
  • 84 microRNAs specifically linked to cancer
  • 3 microRNA reference genes: miR-103-3p, miR-191-5p, miR-423-5p
  • snRNA reference genes: SNORD49A, U6 snRNA, SNORD38B
  • 3 Inter-plate calibrators (UniSp3 IPC)
  • 5 RNA spike-in (cel-miR-39-3p, UniSp2, UnuSp4, UniSp5 and UniSp6)
The Cancer Focus microRNA PCR Panels are compatible with most real-time PCR instruments (Figure 1).


Figure 1
Instrument coverage of the panels. (Click to learn more)

Table 1
Colorectal
Lung
Pancreatic
Stomach
Esophagus
Liver (HCC and CCAs)
Breast
Melanoma
Kidney
Bladder
Brain
Cervical
Adrenal
Prostate
Endometrial
Gallbladder B-cell
Hodgkin’s Lymphomas
Ovarian
Uterine
Sarcoma
GIST
Thyroid Hurthle and follicular cell
Papillary thyroid

Some of the cancer types covered by the panel

Identify microRNA biomarkers or new classifiers

Cancer Focus microRNA PCR Panels can be used for microRNA profiling for many types of cancer from clinical samples including FFPE and biofluids. These microRNA profiles can be used to identify unique cancer signatures and help researchers and clinicians to identify biomarkers or classifiers for cancer types and progression (Figure 1 & 2).

Figure 1 MicroRNA profiles from six types of cancer compared
MicroRNA profiles from six types of cancer compared. (Click to learn more)

Figure 2 Schematic outline of the miRCURY
LNA™ Universal RT microRNA PCR System
microRNA expression signatures from 4 types of cancer. (Click to learn more)

A unique system developed specifically for microRNA profiling

The miRCURY LNA™ Universal RT microRNA PCR System offers the best available combination of performance and ease-of-use on the market because it unites two important features (Figure 2):
  • Universal RT — One single first strand cDNA synthesis reaction can be used as template for multiple microRNA real time PCR assays, regardless of the number of microRNAs being profiled. This saves precious sample, reduces technical variation and saves time in the laboratory.
  • LNA™ PCR amplification — The two microRNA-specific qPCR primers are optimized with LNA™. LNA™ primers bind with high affinity, and are shorter than standard PCR primers, so that both primers can fit on the microRNA without overlapping. The result is unrivalled sensitivity and specificity, and extremely low background.

Unmatched sensitivity

Universal RT combined with LNA™-enhanced and Tm normalized primers enables accurate and reliable quantification of individual microRNAs from as little as 1 pg total RNA (Figure 3). In comparison to other microRNA real-time PCR systems using either stem-loop or standard DNA primers, the LNA™-enhanced primers offer significantly increased sensitivity, especially for AU-rich microRNAs.

Exceptional sensitivity as well as extremely low background enables accurate quantification of very low levels of microRNA without the need for pre-amplification. This makes the miRCURY LNA™ Universal RT microRNA PCR System suitable for all sample types, and especially samples with low RNA content e.g. biofluids such as serum/plasma.

Fully validated and optimized

All miRCURY LNA™ Universal RT microRNA PCR primer sets have been optimized for maximum sensitivity and thoroughly validated either by wet lab testing or in silico. In wet lab validation, over 95% of assays detect 10 microRNA copies or less in the PCR reaction (Figure 4). The primer sets have also been validated for specific amplification of the target and for minimal background signal.

Truly specific – no false positives

The incorporation of LNA™ in the qPCR primers facilitates the design of assays that can distinguish between microRNA sequences that differ by a single nucleotide. This makes it a truly specific microRNA qPCR platform – the only microRNA platform that gives no false positive signals (Figure 5). In addition, the assays can discriminate between mature microRNA sequences and precursor microRNA (Table 1).

Fast, easy and reproducible

Save time and effort in the laboratory with the 3 hour easy-to-follow protocol that minimizes pipetting. By using the same cDNA as template in all subsequent PCR reactions, the procedure is greatly simplified compared to systems that require microRNA-specific cDNA synthesis.

With fewer pipetting steps, technical variation is also reduced. As a result, it is possible to achieve extremely high reproducibility from day-to-day (Figure 6) and even site-to-site.

Flexible qPCR system

Tailor your experimental setup to your specific need using individual assays or fully flexible custom Pick-&-Mix panels . Using the same cDNA reaction, shift seamlessly between individual primers, pre-designed miRNome and Focus panels, or custom Pick-&-Mix panels.

Figure 1 Overview of the miRCURY LNA™ Universal RT PCR workflow
Overview of the miRCURY LNA™ Universal RT PCR workflow. (Click to learn more)

Figure 2 Schematic outline of the miRCURY LNA™ Universal RT microRNA PCR System
Schematic outline of the miRCURY LNA™ Universal RT microRNA PCR System. (Click to learn more)

Figure 3 Accurate quantitation from down to 1 pg total RNA starting material
Accurate quantitation from down to 1 pg total RNA starting material. (Click to learn more)

Figure 4 Serial dilution of hsa-miR-181a
Serial dilution of hsa-miR-181a. (Click to learn more)

Figure 5 Specificity results from the miRQC Study
Specificity results from the miRQC Study. (Click to learn more) Table 1 Discrimination between mature and precursor microRNAs
Discrimination between mature and precursor microRNAs. (Click to learn more) Figure 6 Excellent day-to-day reproducibility
Excellent day-to-day reproducibility. (Click to learn more)

microRNA and cancer

There have been numerous studies demonstrating links between microRNA dysregulation and various disease states, including a diverse array of human cancers. MicroRNAs have been shown to act as both oncogenes and tumor suppressors.

The high relative stability of microRNA in common clinical source materials (e.g. FFPE blocks, plasma, urine, saliva, etc.) and the ability of microRNAs to function as molecular markers for discrete tissue types and disease states have positioned microRNA quantification as a promising new tool for a wide range of diagnostic applications. MicroRNAs can be used as disease classifiers for different types of cancers or as biomarkers to monitor the effects of therapy on cancer prognosis and progression.

Exiqon's microRNA qPCR system offers the reliability and robustness needed for clinical samples such as FFPE. Learn more about the miRCURY LNA™ Universal RT microRNA PCR system



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Prod. No.
Product Name
Description Notes
203875 Cancer Focus microRNA PCR Panel, 96 well (V4.RO) miRCURY LNA™ Universal RT microRNA PCR, 4x Ready-to-Use Cancer Focus microRNA PCR Panels. Each panel comprises one 96-well plate with 84 LNA™ microRNA primer sets focusing on cancer relevant human microRNAs, reference candidates and spike-ins. For use with Roche LightCycler 480 instruments. Use with ExiLENT master mix only
203876 Cancer Focus microRNA PCR Panel, 96 well (V4.AF) miRCURY LNA? Universal RT microRNA PCR, 4x Ready-to-Use Cancer Focus microRNA PCR Panels. Each panel comprises one 96-well plate with 84 LNA? microRNA primer sets focusing on cancer relevant human microRNAs, reference candidates and spike-ins. For use with ABI Fast instrument series and ABI StepOne Plus. Use with ExiLENT master mix only
203877 Cancer Focus microRNA PCR Panel, 96 well (V4.MI) miRCURY LNA? Universal RT microRNA PCR, 4x Ready-to-Use Cancer Focus microRNA PCR Panels. Each panel comprises one 96-well plate with 84 LNA? microRNA primer sets focusing on cancer relevant human microRNAs, reference candidates and spike-ins. For use with BioRad iQ series, Eppendorf, Stratagene Mx4000 and AB 7000 series instruments*. Use with ExiLENT master mix only
203878 Cancer Focus microRNA PCR Panel, 96 well (V4.ST) miRCURY LNA™ Universal RT microRNA PCR, 4x Ready-to-Use Cancer Focus microRNA PCR Panels. Each panel comprises one 96-well plate with 84 LNA™ microRNA primer sets focusing on cancer relevant human microRNAs, reference candidates and spike-ins. For use with Stratagene Mx3000P series. Use with ExiLENT master mix only
203883 Cancer Focus microRNA PCR Panel, 384 well (V4.R) miRCURY LNA™ Universal RT microRNA PCR, 8x Ready-to-Use Cancer Focus microRNA PCR Panels supplied in two 384-well PCR plates. Each panel comprises 84 LNA™ microRNA primer sets focusing on cancer relevant human microRNAs, reference candidates and spike-ins. For use with Roche LightCycler 480 instruments. Use with ExiLENT master mix only
203884 Cancer Focus microRNA PCR Panel, 384 well (V4.M) miRCURY LNA™ Universal RT microRNA PCR, 8x Ready-to-Use Cancer Focus microRNA PCR Panels supplied in two 384-well PCR plates. Each panel comprises 84 LNA™ microRNA primer sets focusing on cancer relevant human microRNAs, reference candidates and spike-ins. For use with ABI 7900HT and other instruments. Use with ExiLENT master mix only


NOTICE TO PURCHASER: DISCLAIMER OF LICENSE No license is conveyed with the purchase of this product under any of US Patents Nos. 5,210,015, 5,487,972, 5,804,375, 5,994,056, 6,171,785, 6,214,979, 5,538,848, 5,723,591, 5,876,930, 6,030,787, and 6,258,569, and corresponding patents outside the United States, or any other patents or patent applications, relating to the 5’ Nuclease and dsDNA-Binding Dye Processes. For further information contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
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