For covalent immobilization of functional groups and biomolecules.
The AQ-Link™ technology is based on a photochemical method for covalent immobilization of functional groups and biomolecules. The anthraquinone (AQ) molecule can be immobilized onto all surfaces containing -CHx- groups, e.g., polymeric substrates as well as silanized glass, upon irradiation with soft UV-light (290-400 nm).
Figure 1. AQ-Link™ formation
Benefits of the AQ-Link™ technology
- Broad applicability - compatible with different formats e.g. microplates, PCR tubes, polymeric slides, silanised glass slides, petri dishes, magnetic beads etc
- Low reagent consumption
- Compatibility with aqueous solutions
- Useful for covalent immobilization of biomolecules using a 1- or 2-step immobilization strategy
- Useful for immobilization of functional groups
- Immobilization of oligonucleotides, PCR products, peptides, proteins etc.
- Can render polymer surfaces more hydrophilic
- Compatible with ELISA techniques
- Compatible with ﬂuorescent dyes
- Please contact us regarding availability of the AQ-Link™ Phosphoamidite for convenient introduction of AQ into oligonucleotides
Biomolecules can generally be immobilized using two different approaches (Figure 2). In a one-step immobilization process, the biomolecules are attached to the AQ-molecule before UV light immobilization. In a two-step immobilization process, an AQ-reagent consisting of AQ, a linker and a functional group are immobilized before the chosen biomolecule is immobilized. Figure 2. Biomolecules can be immobilized in two different ways.
The range of applicability of the AQ-Link chemistry is illustrated in the table below. The ability to mix and match the various ligands and solid supports utilized with the AQ-Link™ provides unique opportunities to design optimized application-specific surfaces. Table 1.
||Microarray slides |
|Peptides & proteins
||PCR tubes |
||Test tubes |
||Petri plates |
||Tissue culture ware |
||RS treated glass
||Filtration matrixes |
||Magnetic beads |
For covalent coupling of biotinylated molecules, proteins and peptides and for detection of histidine-tagged and GST fusion proteins (available from Nunc
under the name Immobilizer™) Learn more about AQ-Link™ Microplates
AQ-Link™ Phosphoramidite The AQ-Link™ Phosphoramidite is used for the introduction of the photosensitive AQ in the 5’-end of an oligonucleotide for region-specific immobilization of oligonucleotides onto e.g., microplates.
- Koch et al., Bioconjugate Chem., 2000, 11, 474-483.
- Jacobsen et al., Clin Chem., 2002, 48(4), 657-60. b) Jacobsen et al. Nucleic Acids Res., 2002, 30 (19), e100. c) Ørum et al., Clin. Chem., 1999, 45, 1898-1905.
AQ-Link™ Amino The AQ-Link™ Amino reagent is used for covalent coupling of for example phosphate or carbolic acid containing biomolecules such as polysaccharides and peptides.
- Jauho et al., J. Imm. Met., 2000, 242, 133-143.
AQ-Link™ Carboxylic Acid
The AQ-Link™ Carboxylic Acid reagent is used for covalent coupling of for example amino containing biomolecules such as proteins and peptides.
- Bruun et al., J. Imm. Met., 2000, 240, 133-142.
- Jensen et al., Innovations & Perspectives in Solid Phase Synthesis & Combinatorial Libraries, Fourth International Symposium, 1996, 419-422.
The AQ-Link™ Electrophile reagent is used for activation of polymeric surfaces such as microplates for subsequent reaction with amines or thiols.
- Jauho et al., Prenat.Diagn., 2003, 23, 898-900.
- Jensen et al., Am. J. Pathol., 2004, 164 (4), 1347-1359