Comparison of effectiveness of different knockdown methods. The diagram shows a representative experiment (n = 3) of fold upregulation (relative to the no-oligonucleotide control) of the pMIR-21 reporter (firefly luciferase) upon cotransfection with knockdown oligonucleotides of various origin against hsa-mir-21. Competitors A and B use DNA-based oligonucleotides for the miRNA knockdown. Expression levels in MCF7 cells were measured 60 h after transfection. The results for 2'-OMe are in accordance with published results (Nucleic Acids Research 34: 2294 – 2304). NC: Negative Control.

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