Representative knockdown of microRNAs by miRCURY™ LNA Knockdown probes. Expression levels of the indicated miR reporter constructs carrying a miR complementary region. The no-target control is the parental vector without a miR-target. HeLa cells were cotransfected with miR-reporter (firefly luciferase), pGL4.13-Renilla (for normalization) and miRCURY™ LNA Knockdown probes against the named hsa-mir targets. Expression levels were measured using the Dual-Glow™ Luciferase (Promega). The results show a representative experiment carried out in triplicate.

Mismatch discrimination ability of miRCURY™ LNA Knockdown probes. The figure shows a representative experiment (n = 3), for perfectly matched and mismatched knockdown probes against hsa-mir-21. The fold upregulation of the pMIR-21 luciferase reporter is shown relative to the no-oligonucleotide control upon transfection with various mismatched (MM) knockdown oligonucleotides. Experiments were carried out in HeLa cells, and luciferase expression levels were measured 24 h after transfection using Dual-Glow™ Luciferase.

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