Specific telomere detection using LNA™ FISH probes.
Image kindly provided by Dr. R. W. Dirks, Leiden University Medical Center, Leiden, The Netherlands |
|
|
Comparison of LNA™ (A) and DNA (B) probes in FISH for the human satellite-2 repeat. The use of a Cy3-labelled LNA™ substituted FISH probe results in strong hybridisation signals on chromosomes 1, 9, 15 and 16, respectively, after hybridisation for 30 min at 37°C (A), whereas no signals can be detected with the DNA FISH probe (B).
Images were kindly provided by A. Silahtaroglu, University of Copenhagen, Denmark. |
|
|
| Cytogenetics
Exiqon’s LNA™ technology enables sensitive, fast and specific detection of chromosomal sequences directly on intact chromosomes.
Key benefits:
Short hybridisation time (< 1h) Works on fixed cells and chromosome spreads Multiplex capability Available with different tags Pre-validated probes
Although fluorescence in situ hybridisation (FISH) has proven useful in cytogenetics, it is still a fairly time-consuming method with limitations in sensitivity and resolution. With the high-affinity LNA™ FISH probes from Exiqon, fast, sensitive and specific detection of chromosomal DNA has finally been enabled – and you don’t have to worry about solubility issues!
For further information please view the material in the Documentation Box to the right.
|
| PublicationsTechnical notes |
|
|
.jpg)
