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figure 1


hsa-miR-21 detection in tissue sections using a miRCURY LNATM microRNA detection probe with a double DIG* (5’ and 3’) label at 40nM (A) or a single 3’ DIG label at 80nM (B). The double DIG probe gives a more intense signal as well as lower background signal, even at lower probe concentration.

Figure 2

Exiqon zebrafish

Specific detection of miR-122a (top), miR-206 (middle) and miR-124a (bottom) using miRCURY LNA™ microRNA Detection Probes for in situ hybridization of whole-mount zebrafish embryos. Part of a complete catalog of images showing the temporal and spacial expression patterns of 115 conserved microRNAs in zebrafish embryos. The images were kindly provided by Dr. Ronald Plasterk, Hubrecht Laboratory, The Netherlands.

Figure 3

Gallus gallus

Specific detection of mir-206 in Gallus gallus embryo using a miRCURY LNA™ microRNA detection probe in situ hybridization. Mir-206 is expressed in all skeletal muscle cells, appearing at the onset of myogenic cell differentiation. At the pictured stage in embryonic development, mir-206 is detected in the myotomal muscle cells.

Figure 4

Exiqon Adult mouse liver

In situ hybridization of the liver-specific miR-122a (red) in mouse cells using miRCURY LNA™ microRNA Detection Probes. DNA is labeled with DAPI (in blue). The image was kindly provided by Dr. Javier Martinez, IMBA - Institute of Molecular Biotechnology, Vienna, Austria.

Figure 5

miR-124a expression in temporal isocortex of human brain detected in formalin fixed paraffin embedded sections. The image was kindly contributed by Dr. Zissimos Mourelatos, UPenn, Philidelphia, USA.

miR-124a expression in temporal isocortex of human brain detected in formalin-fixed, paraffin-embedded sections. The image was kindly contributed by Dr. Zissimos Mourelatos, UPenn, Philadelphia, USA (Nelson PT et al, RNA 2006, 12 (2): 187-191).

MicroRNA 100











MicroRNA-100 expression in Zebrafish whole-mount in situ hybridization

miR-100 expression in zebrafish embryos detected by whole-mount in situ hybridization applying the sensitive miRCURY™ LNA Detection probes (Wienholds E., et al., Science 2005, 309, 310-311). Images show expression in brain (hindbrain, diencephalon); spinal cord. MicroRNA detection products are available from Exiqon, see for example hsa-mir-100. The images were kindly contributed by Dr. Erno Wienholds and Dr. Ronald Plasterk, Hubrecht Laboratory, Utrecht, the Netherlands.
microRNAs (miRNAs) are a class of short endogenous RNAs that act as post-transcriptional modulators of gene expression. To date hundreds of miRNAs have been identifyed in invertebrates, vertebrates and plants. MicroRNAs are currently annotated in the Sanger mir database (see http://microrna.sanger.ac.uk/ ). Growing evidence suggests that miRNAs exhibit a wide variety of regulatory functions and exert significant effects on cell growth, development and differentiation. In addition, recent studies have shown that human miRNA genes are frequently located in cancer-associated genomic regions, while perturbed miRNA expression patterns have been observed in many human cancers.
Exiqon’s unique and proprietary LNA™ (Locked Nucleic Acid) technology enables sensitive and specific detection of miRNAs by in situ hybridisation. Pre-designed or custom miRCURY™ LNA Detection probes for miRNA detection are now available for all known miRNAs in invertebrates, vertebrates and plants. Also, Exiqons miRCURY™ LNA Arrays for microRNA analysis are available either as pre-spotted arrays or as a full service where you only provide the RNA.


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