Figure 3. The table shows the sequences of closely related miR-181 family. miR-181a and c are only distinguished by one nucleotide deletion, and miR-181b and d by one mismatch and two deletions in the end. Graph shows that very little cross hybridization is seen between the family members despite the closely related sequence (shown in the Table).
Synthetic microRNAs were spike into 0.75 µg tRNA and analyzed using the miRCURY™ LNA microRNA Arrays.