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Accurate microRNA quantification over a wide dynamic range of microRNA copy numbers: Hsa-miR-100 (2 x 108 copies) input was used to generate cDNA. A ten-fold serial dilution ranging from 2 x 109 copies to 2 copies cDNA was amplified by real-time PCR. (A) The amplification curves and (B) the standard curve show excellent linear correlation between the decreasing cycle numbers and the logarithm of the microRNA copy number, proving the assay enables sensitive microRNA detection down to less than 10 copies with a wide dynamic range of at least 8 logs. | | |
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