| |
LNA™ in microRNA-specific PCR primers improves the performance the quantitative PCR assay. The sensitivity and dynamic range of assays using DNA primers (blue) versus LNA™-substituted primers (gray) yielded nearly similar results for the miR-194 (A) and the miR-15a (B) assays, but for miR-15a the LNA™ primers were slightly better. (C) For miR-200a, the assay was considerably improved by LNA™ substitutions. (D) The miR-142-3P assay was rescued by using LNA™ primer. Synthetic microRNAs were spiked into a complex background of MS2 Phage total RNA. The microRNA responses are shown as solid lines with no RNA template controls displayed. | | |
|
