Schematic outline of the miRCURY LNA™ microRNA PCR System. Step 1: Directly from total RNA, the microRNA of interest is converted by reverse transcription into a cDNA template using a microRNA-specific RT-primer. Step 2:  The cDNA template is amplified by real-time PCR using a LNA™-enhanced PCR primer anchored in the microRNA sequence together with a universal PCR primer. SYBR® Green is used for detection.

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