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| We handle all sample types including FFPE samples
Whether you wish to submit only a few or several hundred samples we are happy to accept your order. We work with total RNA from all sample types such as cell line cultures, blood, animal tissue and clinical samples.
With every manipulation of your RNA sample there is a risk of introducing an experimental bias. Our platform is not affected by the presence of mRNA and therefore we normally recommend working with total RNA. However, we can also run your experiment with samples enriched for small RNA. In this case, we kindly request you to send us at least 100ng corresponding total RNA per sample for quality control purposes.
Many of our customers express interest in generating microRNA profiles from FFPE samples. We have compared RNA quality and microRNA profiles of samples derived from FFPE and fresh frozen (FrFr) samples prepared from the same tissue. While RNA from FFPE samples was considerably degraded, we still observed very good correlation between microRNA profiles obtained with FFPE and FrFr samples. This suggests that microRNA is much more stabile in FFPE samples than mRNA. The high correlation is a good example of the advantages of our selective LNA™ capture probes and high stringency hybridization conditions, which make our platform robust towards interference from small mRNA degradation products. Please contact us if you are contemplating work with FFPE samples for advice on how best to perform the RNA extraction as not all methods give satisfactory results.
With very large orders we can occasionally assist with sample preparation. Please contact us for more information. |
| PublicationsNature Application Notes |
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