hsa-miR-21 detection in tissue sections using a miRCURY LNATM microRNA detection probe with a double DIG* (5’ and 3’) label at 40nM (A) or a single 3’ DIG label at 80nM (B). The double DIG probe gives a more intense signal as well as lower background signal, even at lower probe concentration. |
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Specific detection of miR-122a (top), miR-206 (middle) and miR-124a (bottom) using miRCURY LNA™ microRNA Detection Probes for in situ hybridization of whole-mount zebrafish embryos. Part of a complete catalog of images showing the temporal and spacial expression patterns of 115 conserved microRNAs in zebrafish embryos. The images were kindly provided by Dr. Ronald Plasterk, Hubrecht Laboratory, The Netherlands. |
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Specific detection of mir-206 in Gallus gallus embryo using a miRCURY LNA™ microRNA detection probe in situ hybridization. Mir-206 is expressed in all skeletal muscle cells, appearing at the onset of myogenic cell differentiation. At the pictured stage in embryonic development, mir-206 is detected in the myotomal muscle cells. |
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In situ hybridization of the liver-specific miR-122a (red) in mouse cells using miRCURY LNA™ microRNA Detection Probes. DNA is labeled with DAPI (in blue). The image was kindly provided by Dr. Javier Martinez, IMBA - Institute of Molecular Biotechnology, Vienna, Austria. |
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miR-124a expression in temporal isocortex of human brain detected in formalin-fixed, paraffin-embedded sections. The image was kindly contributed by Dr. Zissimos Mourelatos, UPenn, Philadelphia, USA (Nelson PT et al, RNA 2006, 12 (2): 187-191). |
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| microRNA detection by in situ hybridization
Now available: Double DIG* labeled detection probes
At a glance
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Detect low abundance microRNAs -
Probes available for all known microRNAs as well as custom sequences -
Single- or dual-base discrimination -
Probes are ready to be labeled or pre-labeled with your preferred label -
Fully developed protocols
Product coverage
Pre-designed miRCURY LNA™ microRNA Detection Probes for in situ hybridization are available for all known microRNAs in invertebrates, vertebrates and plants as registered and annotated in the microRNA registry (miRBase) at the Wellcome Trust Sanger Institute.
Custom detection probes are available for your own microRNAs and other small RNAs, including pre-microRNAs.
Definitively locate when and where
miRCURY LNA™ microRNA Detection Probes have high affinity and discrimination, enabling specific and sensitive detection of microRNAs. Specific in situ detection of microRNA is possible in whole mounts, thin sections, single cells, frozen samples and in formalin-fixed, paraffin-embedded tissue sections (including archived samples).
These detection probes have been used successfully in both animal and plant species. A number of peer-reviewed publications have demonstrated the excellent performance of these probes by showing highly specific microRNA expression pattern in different tissues and cells (Figure 1-5). These probes helps researchers to accurately address “when” and “where” a particular microRNA is expressed. Whenever an optimal signal to noise ratio is required, e.g. to detect low abundance microRNAs, we recommend the combined 5’ and 3’ double DIG* labeled miRCURY LNA™ miRNA detection probes. These are the most sensitive detection probes available due to a cooperative effect of the two DIG* labels. The up to 10-fold higher signal to noise ratio makes the detection of even low expressed targets feasible (see Figure 1). To order Double DIG* miRCURY LNA™ microRNA detection probes, please contact us. The following control miRCURY LNA™ Detection Probes are available: sense-miR-159, scramble-miR and U6. These probes have the same length and LNA™ content as miRCURY LNA™ microRNA Detection Probes and they bear no homology to any known microRNA or mRNA sequence in human, mouse and rat. View prices and labeling options.
Custom miRCURY LNA™ microRNA Detection Probes are also available for your own microRNAs. Please enquire.
All probes are available as ready-to-label or as 3´ or 5´ pre-labeled probes.
For further information, view the material under Documentation to the right.
*Licensed from Roche Diagnostics GmbH
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Protocols
Additional Protocols |
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"I have tried three different techniques for detecting miRNAs by in situ hybridization in human brain tissue - they all failed. When I used the miRCURY probes from Exiqon employing a very simple technique, the protocol instantly and 'painlessly' succeeded. I am very pleased with the results".
says Dr. Peter Nelson from the Mourelatos Lab, UPenn, Philadelphia, PA, USA.
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“We got some beautiful data and spectacular images”.
- Prof Ronald H. A. Plasterk
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