Specific detection of miR-122a (top), miR-206 (middle) and miR-124a (bottom) using miRCURY™ detection probes in in situ hybridisation of whole mount zebrafish embryos.
The images were kindly provided by Dr. Ronald Plasterk, Hubrecht Laboratory, Th |
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Detection of the liver specific miRNA-122a (red) in mouse cells using miRCURY™ detection probes. DNA is labeled with DAPI (in blue).
The image was kindly provided by Dr. Javier Martinez, IMBA - Institute of Molecular Biotechnology, Vienna, Austria |
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miR-124a expression in temporal isocortex of human brain detected in formalin-fixed, paraffin-embedded sections.
The image was kindly contributed by Dr. Zissimos Mourelatos, UPenn, Philadelphia, USA (Nelson PT et al, RNA, 12 (2) 2006). |
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| microRNA Detection by In Situ Hybridization
miRNA expression profiling with miRCURY™ probes: See precisely where your miRNA is expressed with a sensitivity and specificity not possible with normal probes.
Key benefits:
Detect low abundance miRNAs Probes available for all miRNAs Single- or dual-base discrimination Probes are ready to be labeled or pre-labeled with your preferred detection method, e.g. DIG*, biotin, fluorescence Fully developed protocols
*Licensed from Roche Diagnostics GmbH Exiqons miRCURY™ technology enables sensitive and specific detection of mature miRNAs by in situ hybridisation. Customised in situ detection probes for pre-miRs are available upon request.
miRCURY™ in situ detection probes have high binding affinity and discrimination, enabling the specific and sensitive detection of miRNAs. Specific in situ detection of miRNA is possible in whole mounts, thin sections, single cells and in formalin-fixed, paraffin-embedded tissue sections.
Pre-designed miRCURY™ in situ detection probes are available for all known miRNAs in invertebrates, vertebrates and plants, as registered and annotated in the miRNA Registry at The Wellcome Trust Sanger Institute. Please click here to view prices and labeling options.
The following control miRCURY™ detection probes are available: sense-miR-159, scramble-miR and U6. These probes have the same length and LNA content as the miRCURY™ detection probes and they bear no homology to any known miRNA or mRNA sequence in human, mouse and rat. click here to view prices and labeling options.
Custom miRCURY™ detection probes are available for your own miRNAs. Please enquire.
All probes are available as ready-to-label as well as 3´ or 5´ prelabeled probes. For further information, please view the material in the Documentation Box to the right.
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| Publications
Protocols
Additional Protocols |
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"I have tried three different techniques for detecting miRNAs by in situ hybridization in human brain tissue - they all failed. When I used the miRCURY probes from Exiqon employing a very simple technique, the protocol instantly and 'painlessly' succeeded. I am very pleased with the results"
says Dr. Peter Nelson from the Mourelatos Lab, UPenn, Philadelphia, PA, USA.
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“We got some beautiful data and spectacular images”
- Prof Ronald H. A. Plasterk
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