Exiqon - LNA for microRNA (miRNA) research - mRNA in situ hybridisation

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mRNA in situ hybridization

The LNA™ mRNA and small RNA in situ hybridisation probes enable specific detection of mRNAs as well as small RNAs by in situ hybridization with a superior sensitivity and improved specificity compared to longer DNA oligonucleotides and riboprobes.

Key benefits:

Sensitive and specific detection / decreased background
No cloning expertise is needed / simple and fast protocol
Improved target accessibility / good tissue penetration
Several targs available

Figure 1

Detection of SSA4 RNA in ∆rip1 fixed yeast cells using a single labeled Cy3™ LNA™ mRNA in situ hybridisation probe (left figure) or a single labeled Cy3™ DNA oligonucleotide (right figure) of same length showing improved signal and less background staining with the LNA™ mRNA in situ hybridisation probe. Pictures were taken after 20 min hybridisation time in 50% formamide. From Thomsen et al., RNA, Nov 2005; 11(11): 1745 - 1748; reprinted by permission of CSHL Press.

Figure 2

Detection of poly(A)+ RNA in fixed wild-type yeast cells using a single labeled Cy3™ T₂₀ LNA™ in situ hybridisation probe (left figure) at 37ºC giving a nuclear signal that could not be detected using a single labeled Cy3™ T₇₀ DNA oligonucleotide (right figure). The probes have identical T_m. Pictures were taken after 30 min hybridisation time. From Thomsen et al., RNA, Nov 2005; 11(11): 1745 - 1748; reprinted by permission of CSHL Press.

Avoid the hassle with in vitro transcription of riboprobes - use Exiqons high affinity LNA™ mRNA in situ hybridisation probes for specific, fast and sensitive in situ detection of mRNA - including splice variants - in whole mounts, tissue sections, and single or fixed cells.

Figure 3

Fixed Rat 9G cell hybridised with a HCMV-IE mRNA specific LNA™ probe. The bright red dot in the nucleus (stained blue with DAPI) represents the transcription and possibly RNA processing site of HCMV-IE mRNA. The mRNA is also detectable in the cytoplasm seen as red staining.
Image kindly provided by Dr. R. W. Dirks, Leiden University, Leiden, The Netherlands

For further information, please view the material in the Documentation Box to the right.